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Article

SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation

1
Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404394, Taiwan
2
Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404394, Taiwan
3
Department of Molecular Biology and Human Genetics, Tzu Chi University, Hualien 970301, Taiwan
4
Department of Biotechnology, Asia University, Wufeng, Taichung 413305, Taiwan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(9), 3179; https://doi.org/10.3390/ijms21093179
Received: 27 March 2020 / Revised: 23 April 2020 / Accepted: 28 April 2020 / Published: 30 April 2020
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Severe acute respiratory syndrome–associated coronavirus (SARS-CoV) initiates the cytokine/chemokine storm-mediated lung injury. The SARS-CoV unique domain (SUD) with three macrodomains (N, M, and C), showing the G-quadruplex binding activity, was examined the possible role in SARS pathogenesis in this study. The chemokine profile analysis indicated that SARS-CoV SUD significantly up-regulated the expression of CXCL10, CCL5 and interleukin (IL)-1β in human lung epithelial cells and in the lung tissues of the mice intratracheally instilled with the recombinant plasmids. Among the SUD subdomains, SUD-MC substantially activated AP-1-mediated CXCL10 expression in vitro. In the wild type mice, SARS-CoV SUD-MC triggered the pulmonary infiltration of macrophages and monocytes, inducing CXCL10-mediated inflammatory responses and severe diffuse alveolar damage symptoms. Moreover, SUD-MC actuated NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome-dependent pulmonary inflammation, as confirmed by the NLRP3 inflammasome inhibitor and the NLRP3−/− mouse model. This study demonstrated that SARS-CoV SUD modulated NLRP3 inflammasome-dependent CXCL10-mediated pulmonary inflammation, providing the potential therapeutic targets for developing the antiviral agents. View Full-Text
Keywords: SARS-coronavirus; SARS-CoV unique domain (SUD), CXCL10; NLRP3 inflammasome; pulmonary inflammation SARS-coronavirus; SARS-CoV unique domain (SUD), CXCL10; NLRP3 inflammasome; pulmonary inflammation
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MDPI and ACS Style

Chang, Y.-S.; Ko, B.-H.; Ju, J.-C.; Chang, H.-H.; Huang, S.-H.; Lin, C.-W. SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation. Int. J. Mol. Sci. 2020, 21, 3179. https://doi.org/10.3390/ijms21093179

AMA Style

Chang Y-S, Ko B-H, Ju J-C, Chang H-H, Huang S-H, Lin C-W. SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation. International Journal of Molecular Sciences. 2020; 21(9):3179. https://doi.org/10.3390/ijms21093179

Chicago/Turabian Style

Chang, Young-Sheng, Bo-Han Ko, Jyh-Cherng Ju, Hsin-Hou Chang, Su-Hua Huang, and Cheng-Wen Lin. 2020. "SARS Unique Domain (SUD) of Severe Acute Respiratory Syndrome Coronavirus Induces NLRP3 Inflammasome-Dependent CXCL10-Mediated Pulmonary Inflammation" International Journal of Molecular Sciences 21, no. 9: 3179. https://doi.org/10.3390/ijms21093179

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