Influence of Cannabinoid Receptor Deficiency on Parameters Involved in Blood Glucose Regulation in Mice
Abstract
:1. Introduction
2. Results
2.1. Cannabinoid Receptor Expression in Mouse Pancreatic Islets and Islet Cell Types
2.2. Measurement of Body Weight, Blood Glucose, Plasma Insulin and Glucagon
2.3. Measurements of Transcripts and Proteins of Islet Hormones
2.4. Changes in Key Components of the Glucose Sensing Machinery
3. Discussion
4. Materials and Methods
4.1. Animals and Tissue Sampling
4.2. RNA Extraction, DNAse Treatment and Real-Time RT-PCR
4.3. Western Blot
4.4. Immunohistochemistry
4.5. Confocal laser Scanning Microscopy
4.6. Measurements of Plasma Insulin and Glucagon
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CB1 | cannabinoid receptor type 1 |
CB1−/− | CB1-knockout mice |
CB1+/+ | wild-type littermate |
CB2 | cannabinoid receptor type 2 |
CB2−/− | CB2-knockout mice |
ECS | endocannabinoid system |
GPCR | G-protein coupled receptor |
Wt | wild-type |
Appendix A
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Zibolka, J.; Wolf, A.; Rieger, L.; Rothgänger, C.; Jörns, A.; Lutz, B.; Zimmer, A.; Dehghani, F.; Bazwinsky-Wutschke, I. Influence of Cannabinoid Receptor Deficiency on Parameters Involved in Blood Glucose Regulation in Mice. Int. J. Mol. Sci. 2020, 21, 3168. https://doi.org/10.3390/ijms21093168
Zibolka J, Wolf A, Rieger L, Rothgänger C, Jörns A, Lutz B, Zimmer A, Dehghani F, Bazwinsky-Wutschke I. Influence of Cannabinoid Receptor Deficiency on Parameters Involved in Blood Glucose Regulation in Mice. International Journal of Molecular Sciences. 2020; 21(9):3168. https://doi.org/10.3390/ijms21093168
Chicago/Turabian StyleZibolka, Juliane, Anja Wolf, Lisa Rieger, Candy Rothgänger, Anne Jörns, Beat Lutz, Andreas Zimmer, Faramarz Dehghani, and Ivonne Bazwinsky-Wutschke. 2020. "Influence of Cannabinoid Receptor Deficiency on Parameters Involved in Blood Glucose Regulation in Mice" International Journal of Molecular Sciences 21, no. 9: 3168. https://doi.org/10.3390/ijms21093168