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Article

3D Bioprinted Vascularized Tumour for Drug Testing

1
School of Mechanical Engineering, Sungkyunkwan University, Suwon 16419, Korea
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Department of Biomedical Engineering, Sungkyunkwan University, Suwon 16419, Korea
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Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan 50612, Korea
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Korean Medical Science Research Center for Healthy-Aging, Pusan National University, Yangsan 50612, Korea
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Graduate Training Program of Korean Medicine for Healthy-Aging, Pusan National University, Yangsan 50612, Korea
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Department of Pharmacology, School of Medicine, Wonkwang University, Iksan 54538, Korea
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Department of Neurosurgery, Seoul National University Hospital, Seoul 03080, Korea
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Cancer Research Institute, Hypoxia Ischemia Disease Institute, Seoul National University, Seoul 03080, Korea
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Institute of Quantum Biophysics (iQB), Sungkyunkwan University, Suwon 16419, Korea
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Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon 16419, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(8), 2993; https://doi.org/10.3390/ijms21082993
Received: 24 March 2020 / Revised: 16 April 2020 / Accepted: 21 April 2020 / Published: 23 April 2020
An in vitro screening system for anti-cancer drugs cannot exactly reflect the efficacy of drugs in vivo, without mimicking the tumour microenvironment (TME), which comprises cancer cells interacting with blood vessels and fibroblasts. Additionally, the tumour size should be controlled to obtain reliable and quantitative drug responses. Herein, we report a bioprinting method for recapitulating the TME with a controllable spheroid size. The TME was constructed by printing a blood vessel layer consisting of fibroblasts and endothelial cells in gelatine, alginate, and fibrinogen, followed by seeding multicellular tumour spheroids (MCTSs) of glioblastoma cells (U87 MG) onto the blood vessel layer. Under MCTSs, sprouts of blood vessels were generated and surrounding MCTSs thereby increasing the spheroid size. The combined treatment involving the anti-cancer drug temozolomide (TMZ) and the angiogenic inhibitor sunitinib was more effective than TMZ alone for MCTSs surrounded by blood vessels, which indicates the feasibility of the TME for in vitro testing of drug efficacy. These results suggest that the bioprinted vascularized tumour is highly useful for understanding tumour biology, as well as for in vitro drug testing. View Full-Text
Keywords: tumour microenvironment; bioprinting; blood vessel; angiogenesis; fibroblast tumour microenvironment; bioprinting; blood vessel; angiogenesis; fibroblast
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MDPI and ACS Style

Han, S.; Kim, S.; Chen, Z.; Shin, H.K.; Lee, S.-Y.; Moon, H.E.; Paek, S.H.; Park, S. 3D Bioprinted Vascularized Tumour for Drug Testing. Int. J. Mol. Sci. 2020, 21, 2993. https://doi.org/10.3390/ijms21082993

AMA Style

Han S, Kim S, Chen Z, Shin HK, Lee S-Y, Moon HE, Paek SH, Park S. 3D Bioprinted Vascularized Tumour for Drug Testing. International Journal of Molecular Sciences. 2020; 21(8):2993. https://doi.org/10.3390/ijms21082993

Chicago/Turabian Style

Han, Seokgyu, Sein Kim, Zhenzhong Chen, Hwa K. Shin, Seo-Yeon Lee, Hyo E. Moon, Sun H. Paek, and Sungsu Park. 2020. "3D Bioprinted Vascularized Tumour for Drug Testing" International Journal of Molecular Sciences 21, no. 8: 2993. https://doi.org/10.3390/ijms21082993

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