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Upregulation of CASP9 through NF-κB and Its Target MiR-1276 Contributed to TNFα-Promoted Apoptosis of Cancer Cells Induced by Doxorubicin

by Fei Zhou 1,2, Yun Li 1, Yisheng Huang 1, Jian Wu 2, Qinhan Wu 1, Hui Zhu 1,* and Jinke Wang 2,*
1
School of Food Engineering and Biotechnology, Hanshan Normal University, Chaozhou 521041, China
2
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(7), 2290; https://doi.org/10.3390/ijms21072290
Received: 22 February 2020 / Revised: 17 March 2020 / Accepted: 23 March 2020 / Published: 26 March 2020
(This article belongs to the Section Molecular Biology)
Under some conditions, nuclear factor-κB (NF-κB) has a pro-apoptotic role, but the mechanisms underlying this function remain unclear. This study demonstrated that NF-κB directly binds to CASP9 and miR1276 in tumor necrosis factor α (TNFα)-treated HeLa and HepG2 cells. NF-κB upregulated CASP9 expression, whereas downregulated miR1276 expression in the TNFα-treated cells. The miR1276 repressed CASP9 expression in both cells. As a result, a typical NF-κB-mediated coherent feed-forward loop was formed in the TNFα-treated cells. It was proposed that the NF-κB-mediated loop may contribute to cell apoptosis under certain conditions. This opinion was supported by the following evidence: TNFα promoted the apoptosis of HeLa and HepG2 cells induced by doxorubicin (DOX). CASP9 was significantly upregulated and activated by TNFα in the DOX-induced cells. Moreover, a known inhibitor of CASP9 activation significantly repressed the TNFα promotion of apoptosis induced by DOX. These findings indicate that CASP9 is a new mediator of the NF-κB pro-apoptotic pathway, at least in such conditions. This study therefore provides new insights into the pro-apoptotic role of NF-κB. The results also shed new light on the molecular mechanism underlying TNFα-promotion of cancer cells apoptosis induced by some anticancer drugs such as DOX. View Full-Text
Keywords: NF-κB; miR1276; CASP9; apoptosis NF-κB; miR1276; CASP9; apoptosis
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MDPI and ACS Style

Zhou, F.; Li, Y.; Huang, Y.; Wu, J.; Wu, Q.; Zhu, H.; Wang, J. Upregulation of CASP9 through NF-κB and Its Target MiR-1276 Contributed to TNFα-Promoted Apoptosis of Cancer Cells Induced by Doxorubicin. Int. J. Mol. Sci. 2020, 21, 2290.

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