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Recent Advances in Practical Methods for Liver Cell Biology: A Short Overview

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Department of Internal Medicine I, Goethe University Frankfurt, 60590 Frankfurt, Germany
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Institute of Molecular Medicine and Experimental Immunology, University Clinic, 53127 Bonn, Germany
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Department of Internal Medicine I, University Clinic, 53127 Bonn, Germany
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Institute of Biochemistry, Friedrich-Alexander University Erlangen-Nürnberg, 91054 Erlangen, Germany
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Angiogenesis in Liver Disease Research Group, IDIBAPS Biomedical Research Institute, University of Barcelona, 08036 Barcelona, Spain
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Yale University School of Medicine, Yale Liver Center, Department of Internal Medicine, New Haven, CT 06520, USA
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Div. of Gastroenterology and Hepatology, Dept. of Internal Medicine III, Medical University of Vienna, A-1090 Vienna, Austria
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HEPEX Lab for Liver Fibrosis and Portal Hypertension, Medical University of Vienna, A-1090 Vienna, Austria
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Department of Internal Medicine III, University Hospital RWTH Aachen, 52074 Aachen, Germany
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Inserm UMR-1149, Centre de Recherche sur l’Inflammation, 75890 Paris, France
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Sorbonne Paris Cité, Laboratoire d’Excellence Inflamex, Faculté de Médecine, Site Xavier Bichat, Université Paris Diderot, 75018 Paris, France
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Hospitalo-Universitaire UNITY, Service d’Hépatologie, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris, 92110 Clichy, France
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Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University Madrid, Spain
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Department of Hepatology & Gastroenterology, Charité University Medicine Berlin, 10117 Berlin, Germany
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Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry, (IFMPEGKC), University Hospital Aachen, D-52074 Aachen, Germany
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European Foundation for Study of Chronic Liver Failure, 08021 Barcelona, Spain
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Institute of Bioengineering Catalunya, 08028 Barcelona, Spain
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University of Southern Denmark, 5230 Odense, Denmark
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(6), 2027; https://doi.org/10.3390/ijms21062027
Received: 6 February 2020 / Revised: 6 March 2020 / Accepted: 9 March 2020 / Published: 16 March 2020
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany. View Full-Text
Keywords: hepatic stellate cells; hepatocellular cancer; fibrogenesis; steatosis; in vitro models hepatic stellate cells; hepatocellular cancer; fibrogenesis; steatosis; in vitro models
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MDPI and ACS Style

Torres, S.; Abdullah, Z.; Brol, M.J; Hellerbrand, C.; Fernandez, M.; Fiorotto, R.; Klein, S.; Königshofer, P.; Liedtke, C.; Lotersztajn, S.; Nevzorova, Y.A.; Schierwagen, R.; Reiberger, T.; Uschner, F.E.; Tacke, F.; Weiskirchen, R.; Trebicka, J. Recent Advances in Practical Methods for Liver Cell Biology: A Short Overview. Int. J. Mol. Sci. 2020, 21, 2027. https://doi.org/10.3390/ijms21062027

AMA Style

Torres S, Abdullah Z, Brol MJ, Hellerbrand C, Fernandez M, Fiorotto R, Klein S, Königshofer P, Liedtke C, Lotersztajn S, Nevzorova YA, Schierwagen R, Reiberger T, Uschner FE, Tacke F, Weiskirchen R, Trebicka J. Recent Advances in Practical Methods for Liver Cell Biology: A Short Overview. International Journal of Molecular Sciences. 2020; 21(6):2027. https://doi.org/10.3390/ijms21062027

Chicago/Turabian Style

Torres, Sandra; Abdullah, Zeinab; Brol, Maximilian J; Hellerbrand, Claus; Fernandez, Mercedes; Fiorotto, Romina; Klein, Sabine; Königshofer, Philipp; Liedtke, Christian; Lotersztajn, Sophie; Nevzorova, Yulia A.; Schierwagen, Robert; Reiberger, Thomas; Uschner, Frank Erhard; Tacke, Frank; Weiskirchen, Ralf; Trebicka, Jonel. 2020. "Recent Advances in Practical Methods for Liver Cell Biology: A Short Overview" Int. J. Mol. Sci. 21, no. 6: 2027. https://doi.org/10.3390/ijms21062027

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