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Open AccessArticle

Butyrate Decreases ICAM-1 Expression in Human Oral Squamous Cell Carcinoma Cells

1
Department of Oral Biology, School of Dentistry, Medical University of Vienna, Sensengasse 2a, Vienna 1090, Austria
2
Center for Education and Research on Dental Implants (CEPID), Department of Dentistry, School of Dentistry, Federal University of Santa Catarina, Campus Reitor João David Ferreira Lima s/n, Florianopolis – SC 88040-900, Brazil
3
Department of Conservative Dentistry, School of Dentistry, University of Chile, Av. Sergio Livingstone 943, Santiago 7500566, Chile
4
Clinic of Reconstructive Dentistry, University of Zurich, 8032 Zurich, Switzerland
5
Department of Dermatology, Medical University of Vienna, Spitalgasse 23, Vienna 1090, Austria
6
Department of Periodontology, University Bern, Hochschulstrasse 4, 3012 Bern, Switzerland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(5), 1679; https://doi.org/10.3390/ijms21051679
Received: 3 January 2020 / Revised: 20 February 2020 / Accepted: 27 February 2020 / Published: 29 February 2020
(This article belongs to the Special Issue Molecular Mechanisms of Periodontal Disease)
Short-chain fatty acids (SCFA) are bacterial metabolites that can be found in periodontal pockets. The expression of adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) within the epithelium pocket is considered to be a key event for the selective transmigration of leucocytes towards the gingival sulcus. However, the impact of SCFA on ICAM-1 expression by oral epithelial cells remains unclear. We therefore exposed the oral squamous carcinoma cell line HSC-2, primary oral epithelial cells and human gingival fibroblasts to SCFA, namely acetate, propionate and butyrate, and stimulated with known inducers of ICAM-1 such as interleukin-1-beta (IL1β) and tumor necrosis factor-alfa (TNFα). We report here that butyrate but not acetate or propionate significantly suppressed the cytokine-induced ICAM-1 expression in HSC-2 epithelial cells and primary epithelial cells. The G-protein coupled receptor-43 (GPR43/ FFAR2) agonist but not the histone deacetylase inhibitor, trichostatin A, mimicked the butyrate effects. Butyrate also attenuated the nuclear translocation of p65 into the nucleus on HSC-2 cells. The decrease of ICAM-1 was independent of Nrf2/HO-1 signaling and phosphorylation of JNK and p38. Nevertheless, butyrate could not reverse an ongoing cytokine-induced ICAM-1 expression in HSC-2 cells. Overall, these observations suggest that butyrate can attenuate cytokine-induced ICAM-1 expression in cells with epithelial origin. View Full-Text
Keywords: butyric acid; periodontium; intercellular adhesion molecule-1; oral biology; epithelial cells; in vitro butyric acid; periodontium; intercellular adhesion molecule-1; oral biology; epithelial cells; in vitro
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MDPI and ACS Style

Magrin, G.L.; Di Summa, F.; Strauss, F.-J.; Panahipour, L.; Mildner, M.; Magalhães Benfatti, C.A.; Gruber, R. Butyrate Decreases ICAM-1 Expression in Human Oral Squamous Cell Carcinoma Cells. Int. J. Mol. Sci. 2020, 21, 1679. https://doi.org/10.3390/ijms21051679

AMA Style

Magrin GL, Di Summa F, Strauss F-J, Panahipour L, Mildner M, Magalhães Benfatti CA, Gruber R. Butyrate Decreases ICAM-1 Expression in Human Oral Squamous Cell Carcinoma Cells. International Journal of Molecular Sciences. 2020; 21(5):1679. https://doi.org/10.3390/ijms21051679

Chicago/Turabian Style

Magrin, Gabriel L.; Di Summa, Francesca; Strauss, Franz-Josef; Panahipour, Layla; Mildner, Michael; Magalhães Benfatti, Cesar A.; Gruber, Reinhard. 2020. "Butyrate Decreases ICAM-1 Expression in Human Oral Squamous Cell Carcinoma Cells" Int. J. Mol. Sci. 21, no. 5: 1679. https://doi.org/10.3390/ijms21051679

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