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Combined Effect of Light and Nutrients on the Micromorphology of the White rot Fungus Cerrena unicolor

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Department of Biochemistry and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19 St., 20-033 Lublin, Poland
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Department of Botany, Mycology and Ecology, Maria Curie-Skłodowska University, Akademicka 19 St., 20-033 Lublin, Poland
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Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Akademicka 19 St., 20-033 Lublin, Poland
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(5), 1678; https://doi.org/10.3390/ijms21051678
Received: 12 February 2020 / Revised: 23 February 2020 / Accepted: 27 February 2020 / Published: 29 February 2020
(This article belongs to the Section Biochemistry)
Light influences developmental pathways in fungi. Recent transcriptomic and biochemical analyses have demonstrated that light influences the metabolism of a white-rot basidiomycete Cerrena unicolor. However, the expression profile of genes involved in the growth and development, or micromorphological observations of the mycelium in response to variable lighting and culturing media, have not performed. We aim to reveal the effect of light and nutrients on C. unicolor growth and a potential relationship between the culture medium and lighting conditions on fungus micromorphological structures. Confocal laser scanning microscopy and scanning electron microscopy were employed for morphological observations of C. unicolor mycelium cultivated in red, blue, green, and white light and darkness on mineral and sawdust media. A comprehensive analysis of C. unicolor differentially expressed genes (DEGs) was employed to find global changes in the expression profiles of genes putatively involved in light-dependent morphogenesis. Both light and nutrients influenced C. unicolor growth and development. Considerable differences in the micromorphology of the mycelia were found, which were partially reflected in the functional groups of DEGs observed in the fungus transcriptomes. A complex cross-interaction of nutritional and environmental signals on C. unicolor growth and morphology was suggested. The results are a promising starting point for further investigations of fungus photobiology. View Full-Text
Keywords: Light; micromorphology; Cerrena unicolor; arthrospores; sawdust; transcriptomes Light; micromorphology; Cerrena unicolor; arthrospores; sawdust; transcriptomes
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MDPI and ACS Style

Pawlik, A.; Jaszek, M.; Stefaniuk, D.; Świderska-Burek, U.; Mazur, A.; Wielbo, J.; Koper, P.; Żebracki, K.; Janusz, G. Combined Effect of Light and Nutrients on the Micromorphology of the White rot Fungus Cerrena unicolor. Int. J. Mol. Sci. 2020, 21, 1678. https://doi.org/10.3390/ijms21051678

AMA Style

Pawlik A, Jaszek M, Stefaniuk D, Świderska-Burek U, Mazur A, Wielbo J, Koper P, Żebracki K, Janusz G. Combined Effect of Light and Nutrients on the Micromorphology of the White rot Fungus Cerrena unicolor. International Journal of Molecular Sciences. 2020; 21(5):1678. https://doi.org/10.3390/ijms21051678

Chicago/Turabian Style

Pawlik, Anna, Magdalena Jaszek, Dawid Stefaniuk, Urszula Świderska-Burek, Andrzej Mazur, Jerzy Wielbo, Piotr Koper, Kamil Żebracki, and Grzegorz Janusz. 2020. "Combined Effect of Light and Nutrients on the Micromorphology of the White rot Fungus Cerrena unicolor" International Journal of Molecular Sciences 21, no. 5: 1678. https://doi.org/10.3390/ijms21051678

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