Overexpression of the High-Affinity Nitrate Transporter OsNRT2.3b Driven by Different Promoters in Barley Improves Yield and Nutrient Uptake Balance
Abstract
:1. Introduction
2. Results
2.1. Confirmation of Transgenic Barley Lines with Real-Time Quantitative PCR and Western Blotting
2.2. Phenotype of Transgenic Barley Lines during the Vegetative Growth Stage
2.3. Characterization of Transgenic Barley during the Reproductive Growth Stage
2.4. Plant Development and Metabolism at Maturity
2.5. NH4+ and NO3− Influx Rates in WT and OsNRT2.3b Transgenic Barley
2.6. The Effect of Different N Treatments on Gene Expression, and Total N, P, and Other Fe Concentration in Different Plant Parts
2.7. The Characteristics of HvNRT2.5, a Gene Homologous to OsNRT2.3b
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Growth Conditions
4.3. Western Blotting
4.4. DNA/RNA Extraction and qPCR Analysis
4.5. Southern Blot Analysis
4.6. Determination of 15N-NH4+/NO3− Influx Rate in Different Barley Lines
4.7. Analysis of Agronomic Traits
4.8. Measurements of Dry Weight, Nitrate, Total N, and Metal Ion Accumulation
4.9. Cloning and cRNA Synthesis of HvNRT2.5, HvNAR2.1, HvNAR2.2, HvNAR2.3, and AtNPF6.3 (CHL1) and Nitrate Uptake Assay in Xenopus Laevis Oocytes
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Distribution ration of shoot (%) | WT | Ubi-1 | Ubi-2 | Ubi-3 | RSs-1 | RSs-2 | RSs-3 |
Total N | 72.60b | 86.76a | 85.24ab | 84.40ab | 84.10ab | 81.49ab | 81.38ab |
Total P | 66.20b | 79.60a | 78.29a | 75.07a | 74.34a | 83.15a | 82.38a |
Total Fe | 11.49b | 24.22b | 23.87b | 32.24b | 31.48b | 91.16a | 80.79a |
Distribution ration of root (%) | WT | Ubi-1 | Ubi-2 | Ubi-3 | RSs-1 | RSs-2 | RSs-3 |
Total N | 27.40a | 13.24b | 14.76ab | 15.60ab | 15.90ab | 15.81ab | 18.62ab |
Total P | 32.97a | 20.40b | 21.71b | 24.93ab | 25.66ab | 16.85b | 17.62b |
Total Fe | 88.51a | 75.78a | 76.13a | 67.76a | 68.52a | 8.84b | 19.21b |
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Luo, B.; Xu, M.; Zhao, L.; Xie, P.; Chen, Y.; Harwood, W.; Xu, G.; Fan, X.; Miller, A.J. Overexpression of the High-Affinity Nitrate Transporter OsNRT2.3b Driven by Different Promoters in Barley Improves Yield and Nutrient Uptake Balance. Int. J. Mol. Sci. 2020, 21, 1320. https://doi.org/10.3390/ijms21041320
Luo B, Xu M, Zhao L, Xie P, Chen Y, Harwood W, Xu G, Fan X, Miller AJ. Overexpression of the High-Affinity Nitrate Transporter OsNRT2.3b Driven by Different Promoters in Barley Improves Yield and Nutrient Uptake Balance. International Journal of Molecular Sciences. 2020; 21(4):1320. https://doi.org/10.3390/ijms21041320
Chicago/Turabian StyleLuo, Bingbing, Man Xu, Limei Zhao, Peng Xie, Yi Chen, Wendy Harwood, Guohua Xu, Xiaorong Fan, and Anthony J. Miller. 2020. "Overexpression of the High-Affinity Nitrate Transporter OsNRT2.3b Driven by Different Promoters in Barley Improves Yield and Nutrient Uptake Balance" International Journal of Molecular Sciences 21, no. 4: 1320. https://doi.org/10.3390/ijms21041320