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Characterization of the OFP Gene Family and its Putative Involvement of Tuberous Root Shape in Radish

by Yanping Wang 1,†, Qingbiao Wang 1,†, Wei Hao 1, Honghe Sun 2 and Li Zhang 1,*
1
National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
2
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing 100097, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(4), 1293; https://doi.org/10.3390/ijms21041293 (registering DOI)
Received: 13 January 2020 / Revised: 7 February 2020 / Accepted: 10 February 2020 / Published: 14 February 2020
(This article belongs to the Section Molecular Plant Sciences)
The shape of the tuberous root, a very important quality trait, varies dramatically among radish cultivars. Ovate family proteins (OFPs) are plant-specific proteins that regulate multiple aspects of plant growth and development. To investigate the possible role of OFPs in radish tuberous root formation, 35 putative RsOFPs were identified from radish, and their expression patterns were detected during tuberous root development in six different radish cultivars. Phylogenetically, RsOFP2.3 clustered together with AtOFP1 and other members of this family that are known to regulate organ shape. Moreover, RsOFP2.3 expression was negatively correlated with tuberous root elongation after the cortex splitting stage, which made this gene the top candidate for the involvement of tuberous root shape. To further characterize the function of RsOFP2.3, it was ectopically expressed in Arabidopsis. RsOFP2.3 overexpression in Arabidopsis led to multiple phenotypical changes, especially the decreased length and increased width of the hypocotyl. Furthermore, RsOFP2.3 expression was induced by all the five classic plant hormones except ethylene, and it was most sensitive to exogenous gibberellic acid treatment. We also found that RsOFP2.3 was localized in the cytoplasm. Taken together, our results suggested the possible involvement for RsOFP2.3 in suppressing radish tuberous root elongation and that it encodes a functional protein which mainly inhibits the elongation of Arabidopsis aerial organs.
Keywords: ovate family protein; radish; tuberous root; root shape ovate family protein; radish; tuberous root; root shape
MDPI and ACS Style

Wang, Y.; Wang, Q.; Hao, W.; Sun, H.; Zhang, L. Characterization of the OFP Gene Family and its Putative Involvement of Tuberous Root Shape in Radish. Int. J. Mol. Sci. 2020, 21, 1293.

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