Identification and Characterization of circRNAs Responsive to Methyl Jasmonate in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Identification and Characterization of circRNAs
2.2. Validation of circRNAs
2.3. DEcircRNAs Induced by MeJA Treatment
2.4. Functional Prediction of DEcircRNAs by Gene Ontology (GO) Enrichment Analysis
2.5. Functional Prediction of DEcircRNAs Based on the circRNA-miRNA-mRNA Network
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. RNA Extraction, cDNA Library Construction, and RNA Sequencing
4.3. CircRNA Identification and Differential Expression Analysis
4.4. Validation of circRNAs
4.5. Functional Prediction of DEcircRNAs
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Zhang, J.; Liu, R.; Zhu, Y.; Gong, J.; Yin, S.; Sun, P.; Feng, H.; Wang, Q.; Zhao, S.; Wang, Z.; et al. Identification and Characterization of circRNAs Responsive to Methyl Jasmonate in Arabidopsis thaliana. Int. J. Mol. Sci. 2020, 21, 792. https://doi.org/10.3390/ijms21030792
Zhang J, Liu R, Zhu Y, Gong J, Yin S, Sun P, Feng H, Wang Q, Zhao S, Wang Z, et al. Identification and Characterization of circRNAs Responsive to Methyl Jasmonate in Arabidopsis thaliana. International Journal of Molecular Sciences. 2020; 21(3):792. https://doi.org/10.3390/ijms21030792
Chicago/Turabian StyleZhang, Jingjing, Ruiqi Liu, Yanfeng Zhu, Jiaxin Gong, Shuwei Yin, Peisen Sun, Hao Feng, Qi Wang, Shuaijing Zhao, Zhongyuan Wang, and et al. 2020. "Identification and Characterization of circRNAs Responsive to Methyl Jasmonate in Arabidopsis thaliana" International Journal of Molecular Sciences 21, no. 3: 792. https://doi.org/10.3390/ijms21030792