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Article

Identification and Characterization of circRNAs Responsive to Methyl Jasmonate in Arabidopsis thaliana

1
Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi’an 710119, Shaanxi, China
2
College of Life Sciences, Shaanxi Normal University, Xi’an 710119, Shaanxi, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(3), 792; https://doi.org/10.3390/ijms21030792
Received: 11 November 2019 / Revised: 9 January 2020 / Accepted: 23 January 2020 / Published: 25 January 2020
(This article belongs to the Special Issue Chromatin, Epigenetics and Plant Physiology)
Circular RNAs (circRNAs) are endogenous noncoding RNAs with covalently closed continuous loop structures that are formed by 3′–5′ ligation during splicing. These molecules are involved in diverse physiological and developmental processes in eukaryotic cells. Jasmonic acid (JA) is a critical hormonal regulator of plant growth and defense. However, the roles of circRNAs in the JA regulatory network are unclear. In this study, we performed high-throughput sequencing of Arabidopsis thaliana at 24 h, 48 h, and 96 h after methyl JA (MeJA) treatment. A total of 8588 circRNAs, which were distributed on almost all chromosomes, were identified, and the majority of circRNAs had lengths between 200 and 800 bp. We identified 385 differentially expressed circRNAs (DEcircRNAs) by comparing data between MeJA-treated and untreated samples. Gene Ontology (GO) enrichment analysis of the host genes that produced the DEcircRNAs showed that the DEcircRNAs are mainly involved in response to stimulation and metabolism. Additionally, some DEcircRNAs were predicted to act as miRNA decoys. Eight DEcircRNAs were validated by qRT-PCR with divergent primers, and the junction sites of five DEcircRNAs were validated by PCR analysis and Sanger sequencing. Our results provide insight into the potential roles of circRNAs in the MeJA regulation network. View Full-Text
Keywords: circRNAs; jasmonic acid; Arabidopsis thaliana; GO enrichment; miRNA decoys circRNAs; jasmonic acid; Arabidopsis thaliana; GO enrichment; miRNA decoys
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MDPI and ACS Style

Zhang, J.; Liu, R.; Zhu, Y.; Gong, J.; Yin, S.; Sun, P.; Feng, H.; Wang, Q.; Zhao, S.; Wang, Z.; Li, G. Identification and Characterization of circRNAs Responsive to Methyl Jasmonate in Arabidopsis thaliana. Int. J. Mol. Sci. 2020, 21, 792. https://doi.org/10.3390/ijms21030792

AMA Style

Zhang J, Liu R, Zhu Y, Gong J, Yin S, Sun P, Feng H, Wang Q, Zhao S, Wang Z, Li G. Identification and Characterization of circRNAs Responsive to Methyl Jasmonate in Arabidopsis thaliana. International Journal of Molecular Sciences. 2020; 21(3):792. https://doi.org/10.3390/ijms21030792

Chicago/Turabian Style

Zhang, Jingjing, Ruiqi Liu, Yanfeng Zhu, Jiaxin Gong, Shuwei Yin, Peisen Sun, Hao Feng, Qi Wang, Shuaijing Zhao, Zhongyuan Wang, and Guanglin Li. 2020. "Identification and Characterization of circRNAs Responsive to Methyl Jasmonate in Arabidopsis thaliana" International Journal of Molecular Sciences 21, no. 3: 792. https://doi.org/10.3390/ijms21030792

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