The Central Region of Testican-2 Forms a Compact Core and Promotes Cell Migration
Abstract
:1. Introduction
2. Results
2.1. EC Domain of Testican-2 Binds a Single Calcium Ion
2.2. Calcium Binding Stabilizes the Central Core of Testican-2
2.3. The FS–EC–TY Domain Triplet Forms a Compact Structural Core
2.4. Protein Part of Testicans Promotes Cell Migration
3. Discussion
4. Materials and Methods
4.1. Cloning, Expression and Purification
4.2. Calcium-Binding Analysis
4.3. Sequence Analysis and Disorder Prediction
4.4. Small Angle X-ray Scattering and Modeling
4.5. Cell Exclusion Zone Assay
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
BSA | bovine serum albumin |
Dmax | maximum dimension |
EC | extracellular calcium-binding (domain) |
ECM | extracellular matrix |
EOM | ensemble optimization method |
FS | follistatin (domain) |
G | Gibbs free energy |
GAG | glycosaminoglycan |
H | enthaply |
I; I0 | scattered intensity; extrapolated scattering intensity at q = 0 |
ITC | isothermal titration calorimetry |
Kd | dissociation constant |
MT-MMP | membrane-type matrix metaloprotease |
Mw | molecular weight, in Da (daltons) |
PDDF, P(r) | pair distance distribution function |
q | momentum of transfer |
Rg | radius of gyration |
S | entropy |
SAXS | small angle X-ray scattering |
SEC | size-exclusion chromatography |
T | temperature |
TEV | tobbaco etch virus |
TY | thyroglobulin type-1 (domain) |
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Kd [µM] | n | ΔH [kcal/mol] | TΔS [kcal/mol] | ΔG [kcal/mol] |
---|---|---|---|---|
17.1 ± 3.4 | 1.3 ± 0.2 | −17.64 ± 2.4 | 11.25 ± 0.22 | −6.39 |
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Krajnc, A.; Gaber, A.; Lenarčič, B.; Pavšič, M. The Central Region of Testican-2 Forms a Compact Core and Promotes Cell Migration. Int. J. Mol. Sci. 2020, 21, 9413. https://doi.org/10.3390/ijms21249413
Krajnc A, Gaber A, Lenarčič B, Pavšič M. The Central Region of Testican-2 Forms a Compact Core and Promotes Cell Migration. International Journal of Molecular Sciences. 2020; 21(24):9413. https://doi.org/10.3390/ijms21249413
Chicago/Turabian StyleKrajnc, Anja, Aljaž Gaber, Brigita Lenarčič, and Miha Pavšič. 2020. "The Central Region of Testican-2 Forms a Compact Core and Promotes Cell Migration" International Journal of Molecular Sciences 21, no. 24: 9413. https://doi.org/10.3390/ijms21249413