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Article

Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells

1
Department of Molecular Pathology, Nara Medical University, 840 Shijo-cho, Kashihara 634-8521, Nara, Japan
2
Department of Orthopedics, Nara Medical University, 840 Shijo-cho, Kashihara 634-8522, Nara, Japan
3
Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(24), 9347; https://doi.org/10.3390/ijms21249347
Received: 26 November 2020 / Accepted: 6 December 2020 / Published: 8 December 2020
(This article belongs to the Special Issue Attacking Cancer Progression and Metastasis)
Pterostilbene (PTE) is a natural sterbenoid contained in blueberries that has an antioxidant effect. In contrast, PTE also generates oxidative stress in cancer cells and provides an antitumor effect. Here, we examined the potential mechanism of this contrasting effect of PTE using three gastrointestinal cancer cell lines, namely CT26, HT29, and MKN74. PTE showed a dose-dependent inhibition of cell proliferation, sphere-forming ability, and stem cell marker expression in all three cell lines. Furthermore, the cells treated with PTE showed an increase in mitochondrial membrane potential and an increase in mitochondrial oxidative stress and lipid peroxide. Upon concurrent treatment with vitamin E, N-acetyl-L-cysteine, and PTE, the PTE-induced mitochondrial oxidative stress and growth inhibition were suppressed. These findings indicate that PTE induces oxidative stress in cancer cells, suppresses stemness, and inhibits proliferation. These antitumor effects of PTE are considered to be useful in cancer treatment. View Full-Text
Keywords: pterostilbene; cancer; stemness; oxidative stress pterostilbene; cancer; stemness; oxidative stress
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MDPI and ACS Style

Mori, S.; Kishi, S.; Honoki, K.; Fujiwara-Tani, R.; Moriguchi, T.; Sasaki, T.; Fujii, K.; Tsukamoto, S.; Fujii, H.; Kido, A.; Tanaka, Y.; Luo, Y.; Kuniyasu, H. Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells. Int. J. Mol. Sci. 2020, 21, 9347. https://doi.org/10.3390/ijms21249347

AMA Style

Mori S, Kishi S, Honoki K, Fujiwara-Tani R, Moriguchi T, Sasaki T, Fujii K, Tsukamoto S, Fujii H, Kido A, Tanaka Y, Luo Y, Kuniyasu H. Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells. International Journal of Molecular Sciences. 2020; 21(24):9347. https://doi.org/10.3390/ijms21249347

Chicago/Turabian Style

Mori, Shiori, Shingo Kishi, Kanya Honoki, Rina Fujiwara-Tani, Takuma Moriguchi, Takamitsu Sasaki, Kiyomu Fujii, Shinji Tsukamoto, Hiromasa Fujii, Akira Kido, Yasuhito Tanaka, Yi Luo, and Hiroki Kuniyasu. 2020. "Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells" International Journal of Molecular Sciences 21, no. 24: 9347. https://doi.org/10.3390/ijms21249347

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