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Open AccessArticle

Suppression of Ovarian Cancer Cell Growth by AT-MSC Microvesicles

Laboratory of Biology of Stem and Neoplastic Cells, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, 53-114 Wroclaw, Poland
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(23), 9143;
Received: 20 November 2020 / Accepted: 27 November 2020 / Published: 30 November 2020
(This article belongs to the Special Issue Extracellular Vesicles: Biology and Potentials in Cancer Therapeutics)
Transport of bioactive cargo of microvesicles (MVs) into target cells can affect their fate and behavior and change their microenvironment. We assessed the effect of MVs derived from human immortalized mesenchymal stem cells of adipose tissue-origin (HATMSC2-MVs) on the biological activity of the ovarian cancer cell lines ES-2 (clear cell carcinoma) and OAW-42 (cystadenocarcinoma). The HATMSC2-MVs were characterized using dynamic light scattering (DLS), transmission electron microscopy, and flow cytometry. The anti-tumor properties of HATMSC2-MVs were assessed using MTT for metabolic activity and flow cytometry for cell survival, cell cycle progression, and phenotype. The secretion profile of ovarian cancer cells was evaluated with a protein antibody array. Both cell lines internalized HATMSC2-MVs, which was associated with a decreased metabolic activity of cancer cells. HATMSC2-MVs exerted a pro-apoptotic and/or necrotic effect on ES-2 and OAW-42 cells and increased the expression of anti-tumor factors in both cell lines compared to control. In conclusion, we confirmed an effective transfer of HATMSC2-MVs into ovarian cancer cells that resulted in the inhibition of cell proliferation via different pathways, apoptosis and/or necrosis, which, with high likelihood, is related to the presence of different anti-tumor factors secreted by the ES-2 and OAW-42 cells. View Full-Text
Keywords: ovarian cancer cells; ES-2; OAW-42; microvesicles; adipose tissue origin mesenchymal stem cells ovarian cancer cells; ES-2; OAW-42; microvesicles; adipose tissue origin mesenchymal stem cells
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MDPI and ACS Style

Szyposzynska, A.; Bielawska-Pohl, A.; Krawczenko, A.; Doszyn, O.; Paprocka, M.; Klimczak, A. Suppression of Ovarian Cancer Cell Growth by AT-MSC Microvesicles. Int. J. Mol. Sci. 2020, 21, 9143.

AMA Style

Szyposzynska A, Bielawska-Pohl A, Krawczenko A, Doszyn O, Paprocka M, Klimczak A. Suppression of Ovarian Cancer Cell Growth by AT-MSC Microvesicles. International Journal of Molecular Sciences. 2020; 21(23):9143.

Chicago/Turabian Style

Szyposzynska, Agnieszka; Bielawska-Pohl, Aleksandra; Krawczenko, Agnieszka; Doszyn, Olga; Paprocka, Maria; Klimczak, Aleksandra. 2020. "Suppression of Ovarian Cancer Cell Growth by AT-MSC Microvesicles" Int. J. Mol. Sci. 21, no. 23: 9143.

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