Kisspeptin Influences the Reproductive Axis and Circulating Levels of microRNAs in Senegalese Sole
Abstract
:1. Introduction
2. Results
2.1. Kisspeptin Treatment Affects the Reproductive axis
2.2. Circulating sncRNA Profiles in Plasma
2.3. Bioinformatic Prediction of mRNAs Targets by Differentially Expressed (DE) miRNAs
3. Discussion
4. Materials and Methods
4.1. Animals and Housing
4.2. Experimental Design
4.3. Sperm Quality Analysis
4.4. Analysis of Hormones in Blood Plasma
4.5. microRNAs Analysis
4.5.1. Isolation, Libraries Preparation, and Sequencing
4.5.2. Data Processing and Annotations
4.5.3. mRNA Target Prediction
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sampling | Treatment | Live Cells (%) |
---|---|---|
T0 | Kiss | 88.55 ± 12.77 |
Control | 90.47 ± 4.81 | |
2 d | Kiss | 84.87 ± 6.40 |
Control | 89.32 ± 2.96 | |
4 d | Kiss | 89.75 ± 4.26 |
Control | 88.53 ± 1.35 |
miRNA | Sequence | Log2 Fold Change | Average Expression | Adjusted p-Value |
---|---|---|---|---|
let-7e | TGAGGTAGTTGGTTGT | 6.295 | 2.352 | 0.00939 |
miR-199a-3p | AGTAGTCTGCACATTGGTT | 7.973 | 3.232 | 0.00165 |
miR-100-5p | AACCCGTAGATCCGAACTTGTG | −9.335 | 4.472 | 0.00006 |
Kisspeptintreated | Sample ID | Sex | Sampling Point | Raw Reads | Trimmed | Trimmed % | microRNA | microRNA (%) |
A1-1_S1 | Female | 2 d | 453,229 | 208,921 | 46 | 47,725 | 11 | |
A1-2_S2 | Female | 2 d | 500,647 | 237,779 | 47 | 91,581 | 18 | |
A1-3_S3 | Female | 2 d | 567,594 | 288,477 | 51 | 129,980 | 23 | |
A1-4_S4 | Female | 2 d | 475,441 | 209,746 | 44 | 57,941 | 12 | |
A1-11-9_S5 | Female | 2 d | 448,350 | 219,818 | 49 | 25,468 | 6 | |
A1-6_S11 | Male | 2 d | 409,534 | 199,896 | 49 | 57,262 | 14 | |
A1-7_S12 | Male | 2 d | 438,943 | 207,974 | 47 | 57,138 | 13 | |
A1-10_S13 | Male | 2 d | 497,364 | 252,675 | 51 | 63,137 | 13 | |
A1-12_S14 | Male | 2 d | 500,206 | 222,774 | 45 | 48,918 | 10 | |
A1-13_S15 | Male | 2 d | 431,890 | 200,621 | 46 | 35,429 | 8 | |
A1-11-12_S16 | Male | 2 d | 448,502 | 218,492 | 49 | 157,656 | 35 | |
A4-3_S26 | Male | 4 d | 509,277 | 258,979 | 51 | 139,680 | 27 | |
A4-8_S27 | Male | 4 d | 395,813 | 180,599 | 46 | 41,197 | 10 | |
A4-9_S28 | Male | 4 d | 505,458 | 256,239 | 51 | 104,044 | 21 | |
A4-10_S29 | Male | 4 d | 498,680 | 233,040 | 47 | 49,651 | 10 | |
A4-17_S30 | Male | 4 d | 463,867 | 223,033 | 48 | 85,875 | 19 | |
Control untreated | A2-2_S6 | Female | 2 d | 461,160 | 227,736 | 49 | 191,846 | 42 |
A2-5_S7 | Female | 2 d | 491,359 | 243,485 | 50 | 163,635 | 33 | |
A2-7_S8 | Female | 2 d | 415,844 | 192,516 | 46 | 124,752 | 30 | |
A2-9_S9 | Female | 2 d | 475,556 | 211,070 | 44 | 84,252 | 18 | |
A2-10_S10 | Female | 2 d | 443,098 | 218,571 | 49 | 52,960 | 12 | |
A2-11_S17 | Male | 2 d | 496,908 | 245,995 | 50 | 39,267 | 8 | |
A2-12_S18 | Male | 2 d | 488801 | 233,186 | 48 | 65,358 | 13 | |
A2-14_S19 | Male | 2 d | 549,711 | 227,754 | 41 | 29,918 | 5 | |
A2-16_S20 | Male | 2 d | 502,982 | 226,236 | 45 | 41,948 | 8 | |
A3-2_S21 | Male | 4 d | 445,074 | 219,334 | 49 | 47,514 | 11 | |
A3-5_S22 | Male | 4 d | 500,306 | 248,426 | 50 | 93,047 | 19 | |
A3-6_S23 | Male | 4 d | 464,452 | 219,739 | 47 | 98,906 | 21 | |
A3-9_S24 | Male | 4 d | 470,313 | 232,473 | 49 | 133,423 | 28 | |
A3-11_S25 | Male | 4 d | 488,090 | 247,258 | 51 | 31,354 | 6 |
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Oliveira, C.C.V.; Fatsini, E.; Fernández, I.; Anjos, C.; Chauvigné, F.; Cerdà, J.; Mjelle, R.; Fernandes, J.M.O.; Cabrita, E. Kisspeptin Influences the Reproductive Axis and Circulating Levels of microRNAs in Senegalese Sole. Int. J. Mol. Sci. 2020, 21, 9051. https://doi.org/10.3390/ijms21239051
Oliveira CCV, Fatsini E, Fernández I, Anjos C, Chauvigné F, Cerdà J, Mjelle R, Fernandes JMO, Cabrita E. Kisspeptin Influences the Reproductive Axis and Circulating Levels of microRNAs in Senegalese Sole. International Journal of Molecular Sciences. 2020; 21(23):9051. https://doi.org/10.3390/ijms21239051
Chicago/Turabian StyleOliveira, Catarina C. V., Elvira Fatsini, Ignacio Fernández, Catarina Anjos, François Chauvigné, Joan Cerdà, Robin Mjelle, Jorge M. O. Fernandes, and Elsa Cabrita. 2020. "Kisspeptin Influences the Reproductive Axis and Circulating Levels of microRNAs in Senegalese Sole" International Journal of Molecular Sciences 21, no. 23: 9051. https://doi.org/10.3390/ijms21239051