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Article

Yil102c-A is a Functional Homologue of the DPMII Subunit of Dolichyl Phosphate Mannose Synthase in Saccharomyces cerevisiae

Institute of Biochemistry and Biophysics Polish Academy of Sciences, 02-106 Warsaw, Poland
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Int. J. Mol. Sci. 2020, 21(23), 8938; https://doi.org/10.3390/ijms21238938
Received: 6 October 2020 / Revised: 20 November 2020 / Accepted: 23 November 2020 / Published: 25 November 2020
(This article belongs to the Section Molecular Biology)
In a wide range of organisms, dolichyl phosphate mannose (DPM) synthase is a complex of tree proteins Dpm1, Dpm2, and Dpm3. However, in the yeast Saccharomyces cerevisiae, it is believed to be a single Dpm1 protein. The function of Dpm3 is performed in S. cerevisiae by the C-terminal transmembrane domain of the catalytic subunit Dpm1. Until present, the regulatory Dpm2 protein has not been found in S. cerevisiae. In this study, we show that, in fact, the Yil102c-A protein interacts directly with Dpm1 in S. cerevisiae and influences its DPM synthase activity. Deletion of the YIL102c-A gene is lethal, and this phenotype is reversed by the dpm2 gene from Trichoderma reesei. Functional analysis of Yil102c-A revealed that it also interacts with glucosylphosphatidylinositol-N-acetylglucosaminyl transferase (GPI-GnT), similar to DPM2 in human cells. Taken together, these results show that Yil102c-A is a functional homolog of DPMII from T. reesei and DPM2 from humans. View Full-Text
Keywords: Trichoderma reesei; DPM2; GPI-GnT Trichoderma reesei; DPM2; GPI-GnT
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MDPI and ACS Style

Piłsyk, S.; Perlinska-Lenart, U.; Janik, A.; Gryz, E.; Ajchler-Adamska, M.; Kruszewska, J.S. Yil102c-A is a Functional Homologue of the DPMII Subunit of Dolichyl Phosphate Mannose Synthase in Saccharomyces cerevisiae. Int. J. Mol. Sci. 2020, 21, 8938. https://doi.org/10.3390/ijms21238938

AMA Style

Piłsyk S, Perlinska-Lenart U, Janik A, Gryz E, Ajchler-Adamska M, Kruszewska JS. Yil102c-A is a Functional Homologue of the DPMII Subunit of Dolichyl Phosphate Mannose Synthase in Saccharomyces cerevisiae. International Journal of Molecular Sciences. 2020; 21(23):8938. https://doi.org/10.3390/ijms21238938

Chicago/Turabian Style

Piłsyk, Sebastian, Urszula Perlinska-Lenart, Anna Janik, Elżbieta Gryz, Marta Ajchler-Adamska, and Joanna S. Kruszewska. 2020. "Yil102c-A is a Functional Homologue of the DPMII Subunit of Dolichyl Phosphate Mannose Synthase in Saccharomyces cerevisiae" International Journal of Molecular Sciences 21, no. 23: 8938. https://doi.org/10.3390/ijms21238938

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