Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses
Abstract
:1. Introduction
2. Results
2.1. Oxalate Accumulation Induced by Cd and Al Contributes to Inhibit Root Growth in Wild Soybean
2.2. Isolation and Bioinformatics Analysis of the GsAAE3 Gene
2.3. Analysis of the GsAAE3 Expression Pattern
2.4. GsAAE3 Encodes an Oxalyl-CoA Synthetase
2.5. Subcellular Localization of GsAAE3
2.6. Effects of GsAAE3 Overexpression in A. thaliana on the Tolerances to Cd and Al
2.7. Effects of GsAAE3 Overexpression in Soybean Hairy Roots on the Tolerances to Cd and Al
3. Discussion
4. Materials and Methods
4.1. Plant Material and Treatments
4.2. Quantitative Real-Time PCR (qRT-PCR) and Gene Expression Assay
4.3. Bioinformatics Analysis and Statistical Analysis
4.4. Subcellular Localization
4.5. A. thaliana Transformation and Phenotypic Analysis
4.6. Soybean Hairy Root Transformation and Phenotypic Analysis
4.7. GUS Staining
4.8. Root Growth Analysis
4.9. Purification of the Recombinant Protein and Kinetic Analysis
4.10. Oxalate Measurement
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
Cd | Cadmium |
Al | Aluminum |
AAE3 | ACYL-ACTIVATING ENZYME 3 |
GFP | Green fluorescence protein |
qRT-PCR | Real-time quantitative polymerase chain reaction |
CDS | Coding sequence |
WT | Wild-type |
DTT | DL-Dithiothreitol |
ATP | Adenosine triphosphate |
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Xian, P.; Cai, Z.; Cheng, Y.; Lin, R.; Lian, T.; Ma, Q.; Nian, H. Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses. Int. J. Mol. Sci. 2020, 21, 8869. https://doi.org/10.3390/ijms21228869
Xian P, Cai Z, Cheng Y, Lin R, Lian T, Ma Q, Nian H. Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses. International Journal of Molecular Sciences. 2020; 21(22):8869. https://doi.org/10.3390/ijms21228869
Chicago/Turabian StyleXian, Peiqi, Zhandong Cai, Yanbo Cheng, Rongbin Lin, Tengxiang Lian, Qibin Ma, and Hai Nian. 2020. "Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses" International Journal of Molecular Sciences 21, no. 22: 8869. https://doi.org/10.3390/ijms21228869