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Article

Effects of Excess Manganese on the Xylem Sap Protein Profile of Tomato (Solanum lycopersicum) as Revealed by Shotgun Proteomic Analysis

1
Plant Stress Physiology Group, Plant Nutrition Department, Aula Dei Experimental Station, CSIC, P.O. Box 13034, 50080 Zaragoza, Spain
2
United Graduate School of Agricultural Sciences, Iwate University, Morioka 020-8550, Japan
3
School of Biosciences, The University of Melbourne, Parkville, VIC 3052, Australia
4
La Trobe Institute for Agriculture & Food, Department of Animal, Plant & Soil Sciences, AgriBio Building, La Trobe University, Bundoora, VIC 3086, Australia
5
Department of Plant-bioscience, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan
*
Author to whom correspondence should be addressed.
Current address: BIFI, University of Zaragoza, BIFI-IQFR-CSIC Joint Unit, Mariano Esquillor s/n, Campus Rio Ebro, Edificio I+D, 50018 Zaragoza, Spain.
Current address: SCIEX S.L. c/Valgrande 8, Alcobendas, 28108 Madrid, Spain.
§
Current address: Graduate School of Science & Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570, Japan.
Int. J. Mol. Sci. 2020, 21(22), 8863; https://doi.org/10.3390/ijms21228863
Received: 15 October 2020 / Revised: 16 November 2020 / Accepted: 19 November 2020 / Published: 23 November 2020
(This article belongs to the Special Issue Plant Proteomic Research 3.0)
Metal toxicity is a common problem in crop species worldwide. Some metals are naturally toxic, whereas others such as manganese (Mn) are essential micro-nutrients for plant growth but can become toxic when in excess. Changes in the composition of the xylem sap, which is the main pathway for ion transport within the plant, is therefore vital to understanding the plant’s response(s) to metal toxicity. In this study we have assessed the effects of exposure of tomato roots to excess Mn on the protein profile of the xylem sap, using a shotgun proteomics approach. Plants were grown in nutrient solution using 4.6 and 300 µM MnCl2 as control and excess Mn treatments, respectively. This approach yielded 668 proteins reliably identified and quantified. Excess Mn caused statistically significant (at p ≤ 0.05) and biologically relevant changes in relative abundance (≥2-fold increases or ≥50% decreases) in 322 proteins, with 82% of them predicted to be secretory using three different prediction tools, with more decreasing than increasing (181 and 82, respectively), suggesting that this metal stress causes an overall deactivation of metabolic pathways. Processes most affected by excess Mn were in the oxido-reductase, polysaccharide and protein metabolism classes. Excess Mn induced changes in hydrolases and peroxidases involved in cell wall degradation and lignin formation, respectively, consistent with the existence of alterations in the cell wall. Protein turnover was also affected, as indicated by the decrease in proteolytic enzymes and protein synthesis-related proteins. Excess Mn modified the redox environment of the xylem sap, with changes in the abundance of oxido-reductase and defense protein classes indicating a stress scenario. Finally, results indicate that excess Mn decreased the amounts of proteins associated with several signaling pathways, including fasciclin-like arabinogalactan-proteins and lipids, as well as proteases, which may be involved in the release of signaling peptides and protein maturation. The comparison of the proteins changing in abundance in xylem sap and roots indicate the existence of tissue-specific and systemic responses to excess Mn. Data are available via ProteomeXchange with identifier PXD021973. View Full-Text
Keywords: xylem sap; manganese toxicity; proteome; tomato; shotgun proteomics xylem sap; manganese toxicity; proteome; tomato; shotgun proteomics
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MDPI and ACS Style

Ceballos-Laita, L.; Gutierrez-Carbonell, E.; Takahashi, D.; Lonsdale, A.; Abadía, A.; Doblin, M.S.; Bacic, A.; Uemura, M.; Abadía, J.; López-Millán, A.F. Effects of Excess Manganese on the Xylem Sap Protein Profile of Tomato (Solanum lycopersicum) as Revealed by Shotgun Proteomic Analysis. Int. J. Mol. Sci. 2020, 21, 8863. https://doi.org/10.3390/ijms21228863

AMA Style

Ceballos-Laita L, Gutierrez-Carbonell E, Takahashi D, Lonsdale A, Abadía A, Doblin MS, Bacic A, Uemura M, Abadía J, López-Millán AF. Effects of Excess Manganese on the Xylem Sap Protein Profile of Tomato (Solanum lycopersicum) as Revealed by Shotgun Proteomic Analysis. International Journal of Molecular Sciences. 2020; 21(22):8863. https://doi.org/10.3390/ijms21228863

Chicago/Turabian Style

Ceballos-Laita, Laura, Elain Gutierrez-Carbonell, Daisuke Takahashi, Andrew Lonsdale, Anunciación Abadía, Monika S. Doblin, Antony Bacic, Matsuo Uemura, Javier Abadía, and Ana F. López-Millán 2020. "Effects of Excess Manganese on the Xylem Sap Protein Profile of Tomato (Solanum lycopersicum) as Revealed by Shotgun Proteomic Analysis" International Journal of Molecular Sciences 21, no. 22: 8863. https://doi.org/10.3390/ijms21228863

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