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Article

Generation of Cochlear Hair Cells from Sox2 Positive Supporting Cells via DNA Demethylation

by 1 and 1,2,*
1
Department of Otolaryngology-Head and Neck Surgery, Wayne State University, Detroit, MI 48201, USA
2
John D. Dingell VA Medical Center, Detroit, MI 48201, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(22), 8649; https://doi.org/10.3390/ijms21228649
Received: 28 September 2020 / Revised: 4 November 2020 / Accepted: 13 November 2020 / Published: 17 November 2020
(This article belongs to the Special Issue The Mechanistic Link between Cell Therapy and Neurorehabilitation)
Regeneration of auditory hair cells in adult mammals is challenging. It is also difficult to track the sources of regenerated hair cells, especially in vivo. Previous paper found newly generated hair cells in deafened mouse by injecting a DNA methyltransferase inhibitor 5-azacytidine into the inner ear. This paper aims to investigate the cell sources of new hair cells. Transgenic mice with enhanced green fluorescent protein (EGFP) expression controlled by the Sox2 gene were used in the study. A combination of kanamycin and furosemide was applied to deafen adult mice, which received 4 mM 5-azacytidine injection into the inner ear three days later. Mice were followed for 3, 5, 7 and 14 days after surgery to track hair cell regeneration. Immunostaining of Myosin VIIa and EGFP signals were used to track the fate of Sox2-expressing supporting cells. The results show that (i) expression of EGFP in the transgenic mice colocalized the supporting cells in the organ of Corti, and (ii) the cell source of regenerated hair cells following 5-azacytidine treatment may be supporting cells during 5–7 days post 5-azacytidine injection. In conclusion, 5-azacytidine may promote the conversion of supporting cells to hair cells in chemically deafened adult mice. View Full-Text
Keywords: 5-azacytidine; cell conversion; epigenetics; hair cells; regeneration; supporting cells 5-azacytidine; cell conversion; epigenetics; hair cells; regeneration; supporting cells
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MDPI and ACS Style

Deng, X.; Hu, Z. Generation of Cochlear Hair Cells from Sox2 Positive Supporting Cells via DNA Demethylation. Int. J. Mol. Sci. 2020, 21, 8649. https://doi.org/10.3390/ijms21228649

AMA Style

Deng X, Hu Z. Generation of Cochlear Hair Cells from Sox2 Positive Supporting Cells via DNA Demethylation. International Journal of Molecular Sciences. 2020; 21(22):8649. https://doi.org/10.3390/ijms21228649

Chicago/Turabian Style

Deng, Xin, and Zhengqing Hu. 2020. "Generation of Cochlear Hair Cells from Sox2 Positive Supporting Cells via DNA Demethylation" International Journal of Molecular Sciences 21, no. 22: 8649. https://doi.org/10.3390/ijms21228649

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