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Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut (Castanea sativa) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It

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Phytophthora Research Centre, Department of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, Zemědělská 3, 61300 Brno, Czech Republic
2
Phytophthora Research Centre, Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemědělská 3, 61300 Brno, Czech Republic
3
Forest Protection, Department of Forestry, Faculty of Forestry, University of Belgrade, Kneza Višeslava 1, 11030 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(22), 8525; https://doi.org/10.3390/ijms21228525
Received: 9 October 2020 / Revised: 9 November 2020 / Accepted: 10 November 2020 / Published: 12 November 2020
Phytophthora cinnamomi is one of the most invasive tree pathogens that devastates wild and cultivated forests. Due to its wide host range, knowledge of the infection process at the molecular level is lacking for most of its tree hosts. To expand the repertoire of studied Phytophthora–woody plant interactions and identify molecular mechanisms that can facilitate discovery of novel ways to control its spread and damaging effects, we focused on the interaction between P. cinnamomi and sweet chestnut (Castanea sativa), an economically important tree for the wood processing industry. By using a combination of proteomics, metabolomics, and targeted hormonal analysis, we mapped the effects of P. cinnamomi attack on stem tissues immediately bordering the infection site and away from it. P. cinnamomi led to a massive reprogramming of the chestnut proteome and accumulation of the stress-related hormones salicylic acid (SA) and jasmonic acid (JA), indicating that stem inoculation can be used as an easily accessible model system to identify novel molecular players in P. cinnamomi pathogenicity. View Full-Text
Keywords: sweet chestnut; Phytophthora cinnamomi; proteomics; metabolomics sweet chestnut; Phytophthora cinnamomi; proteomics; metabolomics
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MDPI and ACS Style

Saiz-Fernández, I.; Milenković, I.; Berka, M.; Černý, M.; Tomšovský, M.; Brzobohatý, B.; Kerchev, P. Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut (Castanea sativa) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It. Int. J. Mol. Sci. 2020, 21, 8525. https://doi.org/10.3390/ijms21228525

AMA Style

Saiz-Fernández I, Milenković I, Berka M, Černý M, Tomšovský M, Brzobohatý B, Kerchev P. Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut (Castanea sativa) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It. International Journal of Molecular Sciences. 2020; 21(22):8525. https://doi.org/10.3390/ijms21228525

Chicago/Turabian Style

Saiz-Fernández, Iñigo, Ivan Milenković, Miroslav Berka, Martin Černý, Michal Tomšovský, Břetislav Brzobohatý, and Pavel Kerchev. 2020. "Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut (Castanea sativa) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It" International Journal of Molecular Sciences 21, no. 22: 8525. https://doi.org/10.3390/ijms21228525

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