Letrozole Suppresses the Fusion of Osteoclast Precursors through Inhibition of p38-Mediated DC-STAMP Pathway
Abstract
:1. Introduction
2. Results
2.1. Letrozole Demonstrated No Cytotoxicity on Mouse Bone Marrow-Derived Macrophages (BMMs) and Did Not Alter BMM Proliferation
2.2. Letrozole Inhibited RANKL-Induced Osteoclastogenesis of BMMs
2.3. Letrozole Decreased Osteoclastic Bone Resorption But Did Not Affect Cell Migration
2.4. Letrozole Reduced the mRNA Expression of TRAP, CTR, CTK, and DC-STAMP
2.5. Letrozole Did Not Affect the Expression of Osteoclastogenic Transcription Factors, NFATc1, c-Fos, or the Activation of NF-κB
2.6. Letrozole Diminished p38 Activation in RANKL-Stimulated BMMs
2.7. Anti-Osteoclastogenic Effect of Letrozole Was Abolished by p38 Activation
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. In Vitro Osteoclastogenesis
4.3. MTT Assay
4.4. Resorption Pit Formation Assay
4.5. Trans-Well Migration Assay
4.6. RT-PCR and Quantitative Real-Time PCR Analyses
4.7. Western Blotting
4.8. Statistics
Author Contributions
Funding
Conflicts of Interest
References
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Kim, H.J.; Seong, H.-S.; Choi, Y.; Heo, S.C.; Kim, Y.-D. Letrozole Suppresses the Fusion of Osteoclast Precursors through Inhibition of p38-Mediated DC-STAMP Pathway. Int. J. Mol. Sci. 2020, 21, 8396. https://doi.org/10.3390/ijms21218396
Kim HJ, Seong H-S, Choi Y, Heo SC, Kim Y-D. Letrozole Suppresses the Fusion of Osteoclast Precursors through Inhibition of p38-Mediated DC-STAMP Pathway. International Journal of Molecular Sciences. 2020; 21(21):8396. https://doi.org/10.3390/ijms21218396
Chicago/Turabian StyleKim, Hyung Joon, Hwa-Sik Seong, YunJeong Choi, Soon Chul Heo, and Yong-Deok Kim. 2020. "Letrozole Suppresses the Fusion of Osteoclast Precursors through Inhibition of p38-Mediated DC-STAMP Pathway" International Journal of Molecular Sciences 21, no. 21: 8396. https://doi.org/10.3390/ijms21218396