Genome-Wide Identification of the Tify Gene Family and Their Expression Profiles in Response to Biotic and Abiotic Stresses in Tea Plants (Camellia sinensis)
Abstract
:1. Introduction
2. Results
2.1. Identification of the Members of the TIFY Gene Family in the Tea Plant
2.2. Phylogenetic Analysis of the TIFY Family
2.3. Sequence Analysis of the CsTIFY Family
2.4. Expression Profile of CsTIFY Genes in Different Organs
2.5. Expression Profiles of CsTIFY Genes under JA and Mechanical Wounding Treatment
2.6. Expression Profiles of CsTIFY Genes in Response to Attack by the Tea Geometrid and Tea Anthracnose
2.7. Interactions of CsJAZ with CsMYC2
3. Discussion
4. Materials and Methods
4.1. Insect and Fungi Pathogen
4.2. Plant Materials and Treatments
4.2.1. Different Organs
4.2.2. JA Treatment
4.2.3. Mechanical Wounding Treatment
4.2.4. E. obliqua Treatment
4.2.5. C. camelliae Infection
4.3. Identification of TIFY Family in Tea
4.4. Bioinformatics Analysis of TIFY Family in Tea
4.5. RNA Extraction and qRT-PCR Anlaysis
4.6. Yeast Two-Hybrid Assays
4.7. Data Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene ID | Gene Name | CDS Length (bp) | Protein Length (aa) | TIFY Motif |
---|---|---|---|---|
CSS0010510 | CsJAZ1 | 702 | 233 | TIFYGG |
CSS0031905 | CsJAZ2 | 885 | 294 | TIFYAG |
CSS0022053 | CsJAZ3 | 1197 | 398 | TIFYAG |
CSS0012514 | CsJAZ4 | 1197 | 398 | TIFYAG |
CSS0028188 | CsJAZ7 | 411 | 136 | TIFYNG |
CSS0034430 | CsJAZ8 | 654 | 217 | TIFYNG |
CSS0011019 | CsJAZ10 | 786 | 361 | TIFYGG |
CSS0031620 | CsPPD | 1008 | 335 | TIFYCG |
CSS0050449 | CsZML7 | 912 | 303 | TLSFQG |
CSS0037100 | CsZML8 | 912 | 303 | TLSFQG |
CSS0003047 | CsZML1 | 846 | 281 | TLSFRG |
CSS0017505 | CsZML2 | 972 | 323 | TLSFQG |
CSS0004261 | CsZML3 | 978 | 325 | TLSFQG |
CSS0050427 | CsZML4 | 1092 | 363 | TLAFEG |
CSS0045061 | CsZML5 | 1089 | 362 | TSELSI |
CSS0016802 | CsZML6 | 1131 | 376 | TLAFEG |
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Zhang, X.; Ran, W.; Zhang, J.; Ye, M.; Lin, S.; Li, X.; Sultana, R.; Sun, X. Genome-Wide Identification of the Tify Gene Family and Their Expression Profiles in Response to Biotic and Abiotic Stresses in Tea Plants (Camellia sinensis). Int. J. Mol. Sci. 2020, 21, 8316. https://doi.org/10.3390/ijms21218316
Zhang X, Ran W, Zhang J, Ye M, Lin S, Li X, Sultana R, Sun X. Genome-Wide Identification of the Tify Gene Family and Their Expression Profiles in Response to Biotic and Abiotic Stresses in Tea Plants (Camellia sinensis). International Journal of Molecular Sciences. 2020; 21(21):8316. https://doi.org/10.3390/ijms21218316
Chicago/Turabian StyleZhang, Xin, Wei Ran, Jin Zhang, Meng Ye, Songbo Lin, Xiwang Li, Riffat Sultana, and Xiaoling Sun. 2020. "Genome-Wide Identification of the Tify Gene Family and Their Expression Profiles in Response to Biotic and Abiotic Stresses in Tea Plants (Camellia sinensis)" International Journal of Molecular Sciences 21, no. 21: 8316. https://doi.org/10.3390/ijms21218316