Effects of Estrogen Receptor and Wnt Signaling Activation on Mechanically Induced Bone Formation in a Mouse Model of Postmenopausal Bone Loss
Abstract
:1. Introduction
2. Results
2.1. Effects of Ovariectomy (OVX) and Estradiol Treatment
2.2. Mechanical Loading Increases Cortical Bone Formation and Activates ER or β-Catenin Signaling in Ovariectomized Mice
2.3. ERα and Wnt/β-Catenin Signaling Interact in the Mechanically Induced Response of Preosteoblastic Cells
2.4. Mechanical Loading Increases Wnt and ERα Target Protein Expression and Activates ERα or β-Catenin Signaling in Preosteoblastic Cells
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Ex Vivo Calibration of the Ulna Loading Regimen and Mechanical Loading In Vivo
4.3. Ovariectomy and Estradiol and Gsk-3β Inhibitor Treatments
4.4. Histomorphometry and Bone Microstructure Measurements
4.5. Mechanical Loading In Vitro
4.6. Co-Immunoprecipitation
4.7. Western Blot Analysis
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameter. | Sham | OVX | OVX+E2 |
---|---|---|---|
Estrogen Level (Serum) | |||
Estrogen in pg/mL | 13.7 ± 4.1 | 9.9 ± 1.2 | 78.7 ± 13.6 bc |
Uterus | |||
Uterus weight in mg | 225.2 ± 80.2 | 66.7 ± 31.8 a | 283.7 ± 41.5 c |
Trabecular Bone (L5) | |||
BV/TV in % | 13.9 ± 1.1 | 11.6 ± 1.3 | 16.8 ± 2.4 bc |
Tb.Th in mm | 0.059 ± 0.002 | 0.058 ± 0.002 | 0.062 ± 0.003 c |
Tb.N in 1/mm | 2.4 ± 0.2 | 2.0 ± 0.2 | 2.7 ± 0.4 c |
Tb.Sp in mm | 0.26 ± 0.02 | 0.30 ± 0.02 | 0.25 ± 0.02 c |
Cortical Bone (Ulna) | |||
Ct.Th in mm | 0.180 ± 0.007 | 0.168 ± 0.007 a | 0.172 ± 0.004 |
Ma.Ar in mm2 | 0.028 ± 0.002 | 0.037 ± 0.001 a | 0.032 ± 0.002 bc |
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Liedert, A.; Nemitz, C.; Haffner-Luntzer, M.; Schick, F.; Jakob, F.; Ignatius, A. Effects of Estrogen Receptor and Wnt Signaling Activation on Mechanically Induced Bone Formation in a Mouse Model of Postmenopausal Bone Loss. Int. J. Mol. Sci. 2020, 21, 8301. https://doi.org/10.3390/ijms21218301
Liedert A, Nemitz C, Haffner-Luntzer M, Schick F, Jakob F, Ignatius A. Effects of Estrogen Receptor and Wnt Signaling Activation on Mechanically Induced Bone Formation in a Mouse Model of Postmenopausal Bone Loss. International Journal of Molecular Sciences. 2020; 21(21):8301. https://doi.org/10.3390/ijms21218301
Chicago/Turabian StyleLiedert, Astrid, Claudia Nemitz, Melanie Haffner-Luntzer, Fabian Schick, Franz Jakob, and Anita Ignatius. 2020. "Effects of Estrogen Receptor and Wnt Signaling Activation on Mechanically Induced Bone Formation in a Mouse Model of Postmenopausal Bone Loss" International Journal of Molecular Sciences 21, no. 21: 8301. https://doi.org/10.3390/ijms21218301