TaDrAp1 and TaDrAp2, Partner Genes of a Transcription Repressor, Coordinate Plant Development and Drought Tolerance in Spelt and Bread Wheat
Abstract
:1. Introduction
2. Results
2.1. Identification of Homeologs TaDrAp1 and TaDrAp2 Genes in Bread Wheat cv. Chinese Spring Using Public Databases
2.2. Molecular-Phylogenetic Analysis of Homeologs TaDrAp1 and TaDrAp2 in Bread Wheat Compared to Other Plant Species and References
2.3. Expression Analysis of TaDrAp1 and TaDrAp2 Genes in Eight Cultivars of Bread Wheat
2.4. Microarray 40K SNP Analysis of Parents and Selected Breeding Lines from Interspecies Hybrid Cross No. 18-6 between Spelt and Bread Wheat
2.5. Genetic Polymorphism and SNP Identification in the Segregating Population No. 18-6 from an Interspecies Cross between Spelt and Bread Wheat
2.6. Amplifluor-Like SNP Genotyping of TaDrAp1 and TaDrAp2 Genes in Segregating Population No. 18-6
3. Discussion
3.1. DrAp1/2 Genes Encode Down-Regulator Associated Proteins in Plants
3.2. TaDrAp1 and TaDrAp2 Genes in Wheat
3.3. Expression Analysis of Dr1 and DrAp1 Genes in Monocot Species in Comparison to Bread Wheat during Plant Ontogenesis, Development, and under Drought
3.4. Genetic Polymorphism in TaDrAp1, TaDrAp2, and TaRht-B1 Genes in Spelt and Bread Wheat using 40K SNP Microarray and Amplifluor-Like SNP Genotyping
3.5. Interaction of TaDrAp1 and TaDrAp2 with Genes Controlling Plant Development, Tolerance to Drought, and Yield Components in Spelt and Bread Wheat
4. Materials and Methods
4.1. Bioinformatic Analysis
4.2. Plant Material
4.3. Drought Experiments in the Field Trial
4.4. DNA Extraction and 40K SNP Microarray
4.5. DNA Extraction and Amplifluor-Like SNP Genotyping
4.6. RNA Extraction, cDNA Synthesis, and qPCR Analysis
4.7. Statistical Treatment
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CBF | CCAAT-binding factor |
CDS | Coding sequence |
Dr1 | Down-regulator |
DrAp1 | Down-regulator associated protein |
GWAS | Genome-wide association study |
IWGSC | International wheat genome sequencing consortium |
NC2 | Negative cofactor 2 |
NF-Y | Nuclear factor Y |
NIL | Near-isogenic lines |
ORF | Open reading frame |
RFU | Relative fluorescence units |
SNP | Single nucleotide polymorphism |
TBP | TATA-box-binding protein |
TF | Transcription factor |
TFIID | Transcription factor IID |
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TaDrAp Genes | Gene ID (TraesCS) | ORF (bp) | Chromosome Location (Strand) | CDS (bp) | Protein (aa) |
---|---|---|---|---|---|
TaDrAp1 | |||||
TaDrAp1-A4 | 4A02G151500 | 5188 | Chr4A: 306,315,444–306,320,631 (−) | 768 | 255 |
TaDrAp1-B4 | 4B02G161200 | 10,031 | Chr4B: 316,654,368–316,664,398 (−) | 765 | 254 |
TaDrAp1-D4 | 4D02G158200 | 4338 | Chr4D: 220,464,822–220,469,159 (−) | 768 | 255 |
TaDrAp2 | |||||
TaDrAp2-A1 | 1A02G310700 | 5490 | Chr1A: 501,702,108–501,707,597 (+) | 852 | 283 |
TaDrAp2-B1 | 1B02G322000 | 5172 | Chr1B: 546,528,780–546,533,951 (+) | 852 | 283 |
TaDrAp2-D1 | 1D02G310200 | 5492 | Chr1D: 406,545,964–406,551,455 (+) | 855 | 284 |
ID | Grain Yield (g/plot) | Plant Height (cm) | SNP Genotyping | ||||||
---|---|---|---|---|---|---|---|---|---|
TaDrAp1-A4 | TaDrAp1-B4 * | TaDrAp1-D4 | TaDrAp2-A1 | TaDrAp2-B1 * | TaDrAp2-D1 | TaRht-B1 | |||
♀ 18-4, spelt | 108 | 71 | N.P. | aa | aa | aa | aa | aa | aa |
♂ 18-5, bread | 99 | 57 | N.P. | bb | bb | bb | bb | bb | bb |
Breeding lines, group 1 | |||||||||
18-6-174 | 319 | 94 | - | ab + bb | aa | ab + bb | bb | aa + ab | aa |
18-6-82 | 281 | 87 | - | bb | bb | bb | bb | aa | aa |
18-6-37 | 240 | 67 | - | ab + bb | ab + bb | ab + bb | bb | bb | ab + bb |
Breeding lines, group 2 | |||||||||
18-6-119 | 174 | 79 | - | aa | bb | aa | ab + bb | bb | aa |
18-6-93 | 166 | 66 | - | aa | aa + ab | aa | bb | aa | ab |
18-6-62 | 131 | 68 | - | aa | ab | aa | aa | bb | bb |
Breeding lines, group 3 | |||||||||
18-6-126 | 115 | 78 | - | ab | bb | aa | aa | bb | aa |
18-6-105 | 94 | 58 | - | aa | aa | bb | aa | aa | bb |
18-6-61 | 80 | 57 | - | aa | ab | ab | aa | aa | bb |
Scaffold, SNP microarray | 51014 | 124156, 109338 | 133452 | 10127, 48187 | 88266, 93878 | 20922, 92741 | 30139, 18668 |
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Zotova, L.; Shamambaeva, N.; Lethola, K.; Alharthi, B.; Vavilova, V.; Smolenskaya, S.E.; Goncharov, N.P.; Kurishbayev, A.; Jatayev, S.; Gupta, N.K.; et al. TaDrAp1 and TaDrAp2, Partner Genes of a Transcription Repressor, Coordinate Plant Development and Drought Tolerance in Spelt and Bread Wheat. Int. J. Mol. Sci. 2020, 21, 8296. https://doi.org/10.3390/ijms21218296
Zotova L, Shamambaeva N, Lethola K, Alharthi B, Vavilova V, Smolenskaya SE, Goncharov NP, Kurishbayev A, Jatayev S, Gupta NK, et al. TaDrAp1 and TaDrAp2, Partner Genes of a Transcription Repressor, Coordinate Plant Development and Drought Tolerance in Spelt and Bread Wheat. International Journal of Molecular Sciences. 2020; 21(21):8296. https://doi.org/10.3390/ijms21218296
Chicago/Turabian StyleZotova, Lyudmila, Nasgul Shamambaeva, Katso Lethola, Badr Alharthi, Valeriya Vavilova, Svetlana E. Smolenskaya, Nikolay P. Goncharov, Akhylbek Kurishbayev, Satyvaldy Jatayev, Narendra K. Gupta, and et al. 2020. "TaDrAp1 and TaDrAp2, Partner Genes of a Transcription Repressor, Coordinate Plant Development and Drought Tolerance in Spelt and Bread Wheat" International Journal of Molecular Sciences 21, no. 21: 8296. https://doi.org/10.3390/ijms21218296