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Article

Revealing Alteration in the Hepatic Glucose Metabolism of Genetically Improved Carp, Jayanti Rohu Labeo rohita Fed a High Carbohydrate Diet Using Transcriptome Sequencing

by
Kiran D. Rasal
1,
Mir Asif Iquebal
2,
Sangita Dixit
1,
Manohar Vasam
1,
Mustafa Raza
2,
Lakshman Sahoo
1,
Sarika Jaiswal
2,
Samiran Nandi
1,
Kanta Das Mahapatra
1,
Avinash Rasal
1,
Uday Kumar Udit
1,
Prem Kumar Meher
1,
Khuntia Murmu
1,
UB Angadi
2,
Anil Rai
2,
Dinesh Kumar
2 and
Jitendra Kumar Sundaray
1,*
1
Fish Genetics and Biotechnology Division, ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar 751 002, India
2
Centre for Agricultural Bioinformatics (CABin), ICAR-Indian Agricultural Statistics Research Institute, Library Avenue, PUSA, New Delhi 110012, India
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(21), 8180; https://doi.org/10.3390/ijms21218180
Received: 11 September 2020 / Revised: 20 October 2020 / Accepted: 21 October 2020 / Published: 31 October 2020
(This article belongs to the Section Molecular Genetics and Genomics)
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Abstract

Although feed cost is the greatest concern in aquaculture, the inclusion of carbohydrates in the fish diet, and their assimilation, are still not well understood in aquaculture species. We identified molecular events that occur due to the inclusion of high carbohydrate levels in the diets of genetically improved ‘Jayanti rohu’ Labeo rohita. To reveal transcriptional changes in the liver of rohu, a feeding experiment was conducted with three doses of gelatinized starch (20% (control), 40%, and 60%). Transcriptome sequencing revealed totals of 15,232 (4464 up- and 4343 down-regulated) and 15,360 (4478 up- and 4171 down-regulated) differentially expressed genes. Up-regulated transcripts associated with glucose metabolisms, such as hexokinase, PHK, glycogen synthase and PGK, were found in fish fed diets with high starch levels. Interestingly, a de novo lipogenesis mechanism was found to be enriched in the livers of treated fish due to up-regulated transcripts such as FAS, ACCα, and PPARγ. The insulin signaling pathways with enriched PPAR and mTOR were identified by Kyoto Encyclopedia of Genes and Genome (KEGG) as a result of high carbohydrates. This work revealed for the first time the atypical regulation transcripts associated with glucose metabolism and lipogenesis in the livers of Jayanti rohu due to the inclusion of high carbohydrate levels in the diet. This study also encourages the exploration of early nutritional programming for enhancing glucose efficiency in carp species, for sustainable and cost-effective aquaculture production. View Full-Text
Keywords: Labeo rohita; transcriptome; gene ontology; KEGG; insulin signaling pathway Labeo rohita; transcriptome; gene ontology; KEGG; insulin signaling pathway
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MDPI and ACS Style

Rasal, K.D.; Iquebal, M.A.; Dixit, S.; Vasam, M.; Raza, M.; Sahoo, L.; Jaiswal, S.; Nandi, S.; Mahapatra, K.D.; Rasal, A.; Udit, U.K.; Meher, P.K.; Murmu, K.; Angadi, U.; Rai, A.; Kumar, D.; Sundaray, J.K. Revealing Alteration in the Hepatic Glucose Metabolism of Genetically Improved Carp, Jayanti Rohu Labeo rohita Fed a High Carbohydrate Diet Using Transcriptome Sequencing. Int. J. Mol. Sci. 2020, 21, 8180. https://doi.org/10.3390/ijms21218180

AMA Style

Rasal KD, Iquebal MA, Dixit S, Vasam M, Raza M, Sahoo L, Jaiswal S, Nandi S, Mahapatra KD, Rasal A, Udit UK, Meher PK, Murmu K, Angadi U, Rai A, Kumar D, Sundaray JK. Revealing Alteration in the Hepatic Glucose Metabolism of Genetically Improved Carp, Jayanti Rohu Labeo rohita Fed a High Carbohydrate Diet Using Transcriptome Sequencing. International Journal of Molecular Sciences. 2020; 21(21):8180. https://doi.org/10.3390/ijms21218180

Chicago/Turabian Style

Rasal, Kiran D., Mir A. Iquebal, Sangita Dixit, Manohar Vasam, Mustafa Raza, Lakshman Sahoo, Sarika Jaiswal, Samiran Nandi, Kanta D. Mahapatra, Avinash Rasal, Uday K. Udit, Prem K. Meher, Khuntia Murmu, UB Angadi, Anil Rai, Dinesh Kumar, and Jitendra K. Sundaray. 2020. "Revealing Alteration in the Hepatic Glucose Metabolism of Genetically Improved Carp, Jayanti Rohu Labeo rohita Fed a High Carbohydrate Diet Using Transcriptome Sequencing" International Journal of Molecular Sciences 21, no. 21: 8180. https://doi.org/10.3390/ijms21218180

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