State-of-the-Art Technologies for Understanding Brassinosteroid Signaling Networks
1
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng 475001, China
2
Department of Basic Courses, Zhejiang University of Water Resources and Electric Power, Hangzhou 310018, China
3
State Key Laboratory of Surface Physics, Multiscale Research Institute of Complex Systems, Department of Physics, Fudan University, Shanghai 200433, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(21), 8179; https://doi.org/10.3390/ijms21218179
Received: 22 September 2020
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Revised: 20 October 2020
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Accepted: 22 October 2020
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Published: 31 October 2020
(This article belongs to the Special Issue Advances in Plant Cell Imaging)
Brassinosteroids, the steroid hormones of plants, control physiological and developmental processes through its signaling pathway. The major brassinosteroid signaling network components, from the receptor to transcription factors, have been identified in the past two decades. The development of biotechnologies has driven the identification of novel brassinosteroid signaling components, even revealing several crosstalks between brassinosteroid and other plant signaling pathways. Herein, we would like to summarize the identification and improvement of several representative brassinosteroid signaling components through the development of new technologies, including brassinosteroid-insensitive 1 (BRI1), BRI1-associated kinase 1 (BAK1), BR-insensitive 2 (BIN2), BRI1 kinase inhibitor 1 (BKI1), BRI1-suppressor 1 (BSU1), BR signaling kinases (BSKs), BRI1 ethyl methanesulfonate suppressor 1 (BES1), and brassinazole resistant 1 (BZR1). Furthermore, improvement of BR signaling knowledge, such as the function of BKI1, BES1 and its homologous through clustered regularly interspaced short palindromic repeats (CRISPR), the regulation of BIN2 through single-molecule methods, and the new in vivo interactors of BIN2 identified by proximity labeling are described. Among these technologies, recent advanced methods proximity labeling and single-molecule methods will be reviewed in detail to provide insights to brassinosteroid and other phytohormone signaling pathway studies.
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MDPI and ACS Style
Wang, H.; Song, S.; Cheng, H.; Tan, Y.-W. State-of-the-Art Technologies for Understanding Brassinosteroid Signaling Networks. Int. J. Mol. Sci. 2020, 21, 8179. https://doi.org/10.3390/ijms21218179
AMA Style
Wang H, Song S, Cheng H, Tan Y-W. State-of-the-Art Technologies for Understanding Brassinosteroid Signaling Networks. International Journal of Molecular Sciences. 2020; 21(21):8179. https://doi.org/10.3390/ijms21218179
Chicago/Turabian StyleWang, Haijiao, Song Song, Huaqiang Cheng, and Yan-Wen Tan. 2020. "State-of-the-Art Technologies for Understanding Brassinosteroid Signaling Networks" International Journal of Molecular Sciences 21, no. 21: 8179. https://doi.org/10.3390/ijms21218179
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