5-Nitro-2,4-Dichloropyrimidine as an Universal Model for Low-Energy Electron Processes Relevant for Radiosensitization
Abstract
:1. Introduction
- 1.
- DEA—dissociaitive electron attachment AB + e− → A + B−,
- 2.
- AEA—asociative electron attachment AB + e− → AB−
- 3.
- DNA sensitization
2. Materials and Methods
2.1. Electron Attachment to Isolated 5-Nitro-2,4-Dichloropyrimidine
2.2. Electron Attachment to Dry and Microhydrated 5-Nitro-2,4-Dichloropyrimidine in Molecular Beam
2.3. Cytotoxicity of 5-Nitro-2,4-Dichloropyrimidine
2.3.1. Cell Lines and Culturing
2.3.2. MTT Assay
3. Results and Discussion
3.1. Parent Anion
3.2. Fragment Anions
3.3. Effect of Microhydration
3.4. Cytotoxicity
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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m/z | Assigned Ion | Peak Energy (eV) | Relative Intensity (Max = 100) | Thermodynamic Threshold (eV) |
---|---|---|---|---|
193 | M | 0 | 1.1 | EAa = 2.122 |
177 | [M – O] | 0 | <0.1 | |
2.7 | 0.1 | 1.336 | ||
163 | [M – NO] | 0 | 100 | −2.902 |
147 | [M – NO] | 0 | 75 | −0.044 |
2 | 4.9 | |||
146 | [M – HNO] | 0 | <0.1 | |
2.5 | <0.1 | 1.222 | ||
4.1 | <0.1 | |||
128 | [M – Cl – NO] | 0 | 0.2 | 0.965 |
127 | [M – HCl– NO] | 0 | <0.1 | |
2.3 | 0.1 | |||
3.9 | <0.1 | |||
126 | [M – Cl – O] | 0 | <0.1 | |
2.7 | 0.1 | |||
8.5 | <0.1 | |||
122 | [M – ClH] | 0 | <0.1 | |
2.6 | 0.1 | 2.425 | ||
111 | [M – HCl – NO] | 2.8 | 1.4 | |
99 | CNCl | 2.8 | 0.3 | |
93 | [M – 2Cl – NO] | 2.9 | 0.1 | |
92 | ClNCHNO | 3.2 | 0.1 | |
90 | ClCHNO | 2.8 sh. | ||
4 | <0.1 | |||
6–9 broad | ||||
71 | ClH | 0 | <0.1 | |
2.8 | 0.1 | 2.758 | ||
3.9 sh. | ||||
70 | Cl | 2.9 sh. | 2.471 | |
3.9 | 0.3 | |||
6–9 broad | ||||
68 | CNO | 0 | <0.1 | |
2.7 | <0.1 | |||
66 | CNO | 2.9 | ||
3.9 sh. | ||||
64 | C3N | 3.9 | 0.2 | |
or ClNH | 5.5 sh. | |||
6–9 broad | ||||
61 | ClCN | 0 | <0.1 | |
3 | 0.1 | |||
3.9 | 0.1 | |||
6.7 | <0.1 | |||
50 | CN | 4 | 0.3 | |
7–10 broad | ||||
46 | NO | 2.8 | 2.3 | |
3.9 sh. | ||||
6.2 sh. | ||||
42 | OCN | 0 | <0.1 | |
3.7 | 0.1 | |||
35 | Cl | 0.15 | 5.4 | |
2.7 | 22 | |||
3.8 sh. | ||||
5–9 broad | ||||
26 | CN | 0 | <0.1 | |
2.8 | 0.2 | |||
3.9 | 0.2 | |||
6.2 sh. |
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Luxford, T.F.M.; Pshenichnyuk, S.A.; Asfandiarov, N.L.; Perečko, T.; Falk, M.; Kočišek, J. 5-Nitro-2,4-Dichloropyrimidine as an Universal Model for Low-Energy Electron Processes Relevant for Radiosensitization. Int. J. Mol. Sci. 2020, 21, 8173. https://doi.org/10.3390/ijms21218173
Luxford TFM, Pshenichnyuk SA, Asfandiarov NL, Perečko T, Falk M, Kočišek J. 5-Nitro-2,4-Dichloropyrimidine as an Universal Model for Low-Energy Electron Processes Relevant for Radiosensitization. International Journal of Molecular Sciences. 2020; 21(21):8173. https://doi.org/10.3390/ijms21218173
Chicago/Turabian StyleLuxford, Thomas F. M., Stanislav A. Pshenichnyuk, Nail L. Asfandiarov, Tomáš Perečko, Martin Falk, and Jaroslav Kočišek. 2020. "5-Nitro-2,4-Dichloropyrimidine as an Universal Model for Low-Energy Electron Processes Relevant for Radiosensitization" International Journal of Molecular Sciences 21, no. 21: 8173. https://doi.org/10.3390/ijms21218173