Distinct Molecular Pattern-Induced Calcium Signatures Lead to Different Downstream Transcriptional Regulations via AtSR1/CAMTA3
Abstract
:1. Introduction
2. Results
2.1. MAMP/DAMP-Induced Ca2+ Transients in Cytosol
2.2. MAMPs/DAMP-Induced Ca2+ Transients in the Nucleus
2.3. MAMP/DAMP-Induced Transcriptional Reprogramming of SA-Regulated Genes and JA-Regulated Genes
2.4. AtSR1/CAMTA3 Mediates Pattern-Triggered Transcriptional Reprograming of SA- and JA-Regulated Genes
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Calcium Measurements in Leaf Discs
4.3. Calcium Measurements in Arabidopsis Leaf Protoplasts
4.4. Chemicals, Buffers, and Elicitors
4.5. RNA Preparation and Real-Time PCR Analysis
4.6. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Yuan, P.; Jewell, J.B.; Behera, S.; Tanaka, K.; Poovaiah, B.W. Distinct Molecular Pattern-Induced Calcium Signatures Lead to Different Downstream Transcriptional Regulations via AtSR1/CAMTA3. Int. J. Mol. Sci. 2020, 21, 8163. https://doi.org/10.3390/ijms21218163
Yuan P, Jewell JB, Behera S, Tanaka K, Poovaiah BW. Distinct Molecular Pattern-Induced Calcium Signatures Lead to Different Downstream Transcriptional Regulations via AtSR1/CAMTA3. International Journal of Molecular Sciences. 2020; 21(21):8163. https://doi.org/10.3390/ijms21218163
Chicago/Turabian StyleYuan, Peiguo, Jeremy B. Jewell, Smrutisanjita Behera, Kiwamu Tanaka, and B. W. Poovaiah. 2020. "Distinct Molecular Pattern-Induced Calcium Signatures Lead to Different Downstream Transcriptional Regulations via AtSR1/CAMTA3" International Journal of Molecular Sciences 21, no. 21: 8163. https://doi.org/10.3390/ijms21218163