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A First Glimpse of the Mexican Fruit Fly Anastrepha ludens (Diptera: Tephritidae) Antenna Morphology and Proteome in Response to a Proteinaceous Attractant

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Red de Estudios Moleculares Avanzados, Clúster Científico y Tecnológico BioMimic®, Instituto de Ecología A.C. (INECOL), Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, Mexico
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Red de Manejo Biorracional de Plagas y Vectores, Clúster Científico y Tecnológico BioMimic®, Instituto de Ecología A.C. (INECOL), Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Veracruz, Mexico
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(21), 8086; https://doi.org/10.3390/ijms21218086
Received: 22 September 2020 / Revised: 25 October 2020 / Accepted: 26 October 2020 / Published: 29 October 2020
(This article belongs to the Section Molecular Biology)
Anastrepha ludens is a key pest of mangoes and citrus from Texas to Costa Rica but the mechanisms of odorant perception in this species are poorly understood. Detection of volatiles in insects occurs mainly in the antenna, where molecules penetrate sensillum pores and link to soluble proteins in the hemolymph until reaching specific odor receptors that trigger signal transduction and lead to behavioral responses. Scrutinizing the molecular foundation of odorant perception in A. ludens is necessary to improve biorational management strategies against this pest. After exposing adults of three maturity stages to a proteinaceous attractant, we studied antennal morphology and comparative proteomic profiles using nano-LC-MS/MS with tandem mass tags combined with synchronous precursor selection (SPS)-MS3. Antennas from newly emerged flies exhibited dense agglomerations of olfactory sensory neurons. We discovered 4618 unique proteins in the antennas of A. ludens and identified some associated with odor signaling, including odorant-binding and calcium signaling related proteins, the odorant receptor co-receptor (Orco), and putative odorant-degrading enzymes. Antennas of sexually immature flies exhibited the most upregulation of odor perception proteins compared to mature flies exposed to the attractant. This is the first report where critical molecular players are linked to the odor perception mechanism of A. ludens. View Full-Text
Keywords: Anastrepha ludens; Tephritidae; proteomics; antennae; odorant binding proteins; attractants/traps Anastrepha ludens; Tephritidae; proteomics; antennae; odorant binding proteins; attractants/traps
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Ruiz-May, E.; Altúzar-Molina, A.; Elizalde-Contreras, J.M.; Arellano-de los Santos, J.; Monribot-Villanueva, J.; Guillén, L.; Vázquez-Rosas-Landa, M.; Ibarra-Laclette, E.; Ramírez-Vázquez, M.; Ortega, R.; Aluja, M. A First Glimpse of the Mexican Fruit Fly Anastrepha ludens (Diptera: Tephritidae) Antenna Morphology and Proteome in Response to a Proteinaceous Attractant. Int. J. Mol. Sci. 2020, 21, 8086.

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