Blood Profile of Cytokines, Chemokines, Growth Factors, and Redox Biomarkers in Response to Different Protocols of Treadmill Running in Rats
Abstract
:1. Introduction
2. Results
2.1. Serum Concentrations of Cytokines in Response to Treadmill Running
2.2. Enzymatic and Non-Enzymatic Antioxidants, Total Antioxidant/Oxidant Status, Oxidative Damage Products, and Nitrosative Stress in Plasma or Serum of Rats Subjected to Treadmill Running
2.3. Plasma Content and Composition of Free Fatty Acids (FFA) in Rats Subjected to Treadmill Running
2.4. Correlations
3. Discussion
4. Materials and Methods
4.1. Animal Experiments
- (1)
- Sedentary control.
- (2)
- Rats running on a treadmill set at +10° incline at the speed of 18 m/min for 30 min (M30).
- (3)
- Rats running on a treadmill set at +10° incline at the speed of 18 m/min for 120 min (M120).
- (4)
- Rats running on a treadmill set at +10° incline at the speed of 28 m/min for 30 min (F30).
4.2. Cytokine/Chemokine Detection in Serum by ELISA Multiplex
4.3. Determination of Redox Biomarkers
4.3.1. Antioxidant Barrier
4.3.2. Redox Status
4.3.3. Oxidative Damage Products
4.3.4. Nitrosative Stress
4.4. Plasma Free Fatty Acid Content and Composition
4.5. Data Analysis and Statistics
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
AGE | advanced glycation end products |
AMPK | AMP-activated protein kinase |
AOPP | advanced oxidation protein products |
CAT | catalase |
FOXO3a | forkhead box O3a |
FRAP | ferric reducing ability of plasma |
G-CSF | granulocyte colony-stimulating factor |
GM-CSF | granulocyte-macrophage colony stimulating factor |
GPx | glutathione peroxidase |
GRO KC/CXCL1 | growth-regulated oncogene/keratinocyte chemoattractant/chemokine (C-X-C motif) ligand 1 |
GSH | reduced glutathione |
HO-1 | heme oxygenase-1 |
IFNγ | interferon γ |
IL | interleukin |
IL-1ra | interleukin-1 receptor antagonist |
JNK | c-Jun N-terminal kinases |
Km | Michaelis–Menten constant |
LOOH | lipid hydroperoxides |
MAA | malondialdehyde acetaldehyde |
MAPK | mitogen activated protein kinase |
MCP1/CCL2 | monocyte chemoattractant protein 1/chemokine (C-C motif) ligand 2 |
M-CSF | macrophage colony-stimulating factor |
MDA | malondialdehyde |
MIP-1α/CCL3 | macrophage inflammatory protein-1α/chemokine (C-C motif) ligand 3 |
MIP-3α/CCL20 | macrophage inflammatory protein-3α/chemokine (C-C motif) ligand 20 |
NADPH | nicotinamide adenine dinucleotide phosphate |
NEFA | non-esterified fatty acids |
NF-κB | nuclear factor κB |
NO | nitric oxide |
NOS | nitric oxide synthase |
Nrf2 | nuclear factor-like 2 |
RAGE | receptor for advanced glycation end products |
RANTES/CCL5 | regulated on activation, normal T-cell expressed and secreted/chemokine (C-C motif) ligand 5 |
SOD | superoxide dismutase |
TNFα | tumor necrosis factor α |
VEGF | vascular endothelial growth factor |
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FFA | Control | M30 | M120 | F30 |
---|---|---|---|---|
SAT | 69.41 (55.90–85.09) | 97.08 (86.00–119.84) * | 242.07 (159.10–279.02) *,$ | 139.10 (128.14–148.59) *,# |
UNSAT | 73.55 (58.64–98.49) | 126.82 (108.18–154.22) * | 183.41 (160.34–211.27) *,$ | 170.25 (148.93–178.21) *,# |
Total | 140.18 (116.72–183.53) | 224.45 (194.18–274.06) * | 401.42 (373.91–458.89) *,$ | 306.85 (289.08–324.31) *,# |
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Supruniuk, E.; Maciejczyk, M.; Zalewska, A.; Górski, J.; Chabowski, A. Blood Profile of Cytokines, Chemokines, Growth Factors, and Redox Biomarkers in Response to Different Protocols of Treadmill Running in Rats. Int. J. Mol. Sci. 2020, 21, 8071. https://doi.org/10.3390/ijms21218071
Supruniuk E, Maciejczyk M, Zalewska A, Górski J, Chabowski A. Blood Profile of Cytokines, Chemokines, Growth Factors, and Redox Biomarkers in Response to Different Protocols of Treadmill Running in Rats. International Journal of Molecular Sciences. 2020; 21(21):8071. https://doi.org/10.3390/ijms21218071
Chicago/Turabian StyleSupruniuk, Elżbieta, Mateusz Maciejczyk, Anna Zalewska, Jan Górski, and Adrian Chabowski. 2020. "Blood Profile of Cytokines, Chemokines, Growth Factors, and Redox Biomarkers in Response to Different Protocols of Treadmill Running in Rats" International Journal of Molecular Sciences 21, no. 21: 8071. https://doi.org/10.3390/ijms21218071