Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice
Abstract
:1. Introduction
2. Results
2.1. Effect of External Ca Concentrations on Net Cd2+ Flux and Distribution of Cd in Roots and Tissue Cd Contents of Rice Plants at Seedling Stage
2.2. Effects of Ca Channel Blockers on Net Cd2+ Flux and Distribution of Cd in Rice Roots and Tissue Cd Contents
2.3. Gene Expression under Different Ca Concentrations or Pharmacological Treatments
2.4. Effects of External Ca Concentration on Plant Growth and Tissue Cd Content in Rice Plants at Tillering and Ripening Stages
3. Discussion
3.1. Ca Plays a Double-Edged Role in Regulating Root Cd Uptake and Root-To-Shoot Cd Translocation in Rice
3.2. Ca Decreases Root Cd Uptake in Rice Not Only by Competing for Absorption Sites but Also by Suppressing the Expression of OsNRAMPs
3.3. Ca Facilitates Root-To-Shoot Cd Translocation in Rice by Modulating the Expression of OsHMAs
3.4. The Potentiality of Ca-Based Amendments for Low-Cd Rice Production
4. Materials and Methods
4.1. Plant Materials, Growth Conditions, and Treatments
4.2. Measurement of Cd Content in Rice Tissues
4.3. Microelectrode Measurements of Net Cd2+ Flux
4.3.1. Procedure of Microelectrode Ion Flux Measurements
4.3.2. Transient Cd2+ Flux Kinetics
4.3.3. Pharmacological Experiments
4.4. Histochemical Detection of Cd in Rice Root
4.5. RNA Extraction and Gene Expression Analysis
4.6. Statistics Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Zhang, S.; Li, Q.; Nazir, M.M.; Ali, S.; Ouyang, Y.; Ye, S.; Zeng, F. Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice. Int. J. Mol. Sci. 2020, 21, 8058. https://doi.org/10.3390/ijms21218058
Zhang S, Li Q, Nazir MM, Ali S, Ouyang Y, Ye S, Zeng F. Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice. International Journal of Molecular Sciences. 2020; 21(21):8058. https://doi.org/10.3390/ijms21218058
Chicago/Turabian StyleZhang, Shuo, Qi Li, Muhammad Mudassir Nazir, Shafaqat Ali, Younan Ouyang, Shuzhen Ye, and Fanrong Zeng. 2020. "Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice" International Journal of Molecular Sciences 21, no. 21: 8058. https://doi.org/10.3390/ijms21218058