Mammalian Homologue NME3 of DYNAMO1 Regulates Peroxisome Division
1
Medical Institute of Bioregulation, Institute of Rheological Functions of Food—Kyushu University Collaboration Program, Kyushu University, 3-1-1 Maidashi, Fukuoka 812-8582, Japan
2
Faculty of Arts and Science, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan
3
Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
4
Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei 10002, Taiwan
5
Galilee Medical Center, Institute of Human Genetics, Nahariya 22100, Israel
6
Azrieli Faculty of Medicine, Bar-Ilan University, Safed 13100, Israel
*
Author to whom correspondence should be addressed.
†
These authors equally contributed to this work.
‡
Present address: Department of Neuroinflammation and Brain Fatigue Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
§
Present address: Institute of Rheological Function of Food, Hisayama-cho, Fukuoka 811-2501, Japan.
Int. J. Mol. Sci. 2020, 21(21), 8040; https://doi.org/10.3390/ijms21218040
Received: 8 September 2020
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Revised: 22 October 2020
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Accepted: 25 October 2020
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Published: 28 October 2020
(This article belongs to the Special Issue Selected Papers from the 11th International Conference on the NME/NDPK/NM23/AWD Gene Family (NME 2019))
Peroxisomes proliferate by sequential processes comprising elongation, constriction, and scission of peroxisomal membrane. It is known that the constriction step is mediated by a GTPase named dynamin-like protein 1 (DLP1) upon efficient loading of GTP. However, mechanism of fuelling GTP to DLP1 remains unknown in mammals. We earlier show that nucleoside diphosphate (NDP) kinase-like protein, termed dynamin-based ring motive-force organizer 1 (DYNAMO1), generates GTP for DLP1 in a red alga, Cyanidioschyzon merolae. In the present study, we identified that nucleoside diphosphate kinase 3 (NME3), a mammalian homologue of DYNAMO1, localizes to peroxisomes. Elongated peroxisomes were observed in cells with suppressed expression of NME3 and fibroblasts from a patient lacking NME3 due to the homozygous mutation at the initiation codon of NME3. Peroxisomes proliferated by elevation of NME3 upon silencing the expression of ATPase family AAA domain containing 1, ATAD1. In the wild-type cells expressing catalytically-inactive NME3, peroxisomes were elongated. These results suggest that NME3 plays an important role in peroxisome division in a manner dependent on its NDP kinase activity. Moreover, the impairment of peroxisome division reduces the level of ether-linked glycerophospholipids, ethanolamine plasmalogens, implying the physiological importance of regulation of peroxisome morphology.
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Keywords:
peroxisome; constriction; NDP kinase; GTP; nme3 patient
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MDPI and ACS Style
Honsho, M.; Abe, Y.; Imoto, Y.; Chang, Z.-F.; Mandel, H.; Falik-Zaccai, T.C.; Fujiki, Y. Mammalian Homologue NME3 of DYNAMO1 Regulates Peroxisome Division. Int. J. Mol. Sci. 2020, 21, 8040. https://doi.org/10.3390/ijms21218040
AMA Style
Honsho M, Abe Y, Imoto Y, Chang Z-F, Mandel H, Falik-Zaccai TC, Fujiki Y. Mammalian Homologue NME3 of DYNAMO1 Regulates Peroxisome Division. International Journal of Molecular Sciences. 2020; 21(21):8040. https://doi.org/10.3390/ijms21218040
Chicago/Turabian StyleHonsho, Masanori, Yuichi Abe, Yuuta Imoto, Zee-Fen Chang, Hanna Mandel, Tzipora C. Falik-Zaccai, and Yukio Fujiki. 2020. "Mammalian Homologue NME3 of DYNAMO1 Regulates Peroxisome Division" International Journal of Molecular Sciences 21, no. 21: 8040. https://doi.org/10.3390/ijms21218040
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