Mammalian Homologue NME3 of DYNAMO1 Regulates Peroxisome Division
Abstract
:1. Introduction
2. Results
2.1. Morphological Changes of Peroxisomes in Cells Reduced Expression of NME3
2.2. Intracellular Localization of NME3
2.3. NME3 Is Elevated by Knockdown of ATAD1
2.4. Peroxisomes Are Increased in Number upon Knocking down ATAD1
2.5. Peroxisomes Are Elongated by Expression of Catalytically Inactive NME3
2.6. Decreased Level of Plasmalogens in F741 Patient Fibroblasts
3. Discussion
4. Materials and Methods
4.1. Cell Culture, DNA Transfection, and RNAi
4.2. Antibodies
4.3. RT-PCR
4.4. Plasmids
4.5. Immunoblotting
4.6. Immunofluorescence Microscopy
4.7. Subcellular Fractionation and Biochemical Analysis
4.8. Lipid Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Statistical Analysis and Data Presentation
References
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Honsho, M.; Abe, Y.; Imoto, Y.; Chang, Z.-F.; Mandel, H.; Falik-Zaccai, T.C.; Fujiki, Y. Mammalian Homologue NME3 of DYNAMO1 Regulates Peroxisome Division. Int. J. Mol. Sci. 2020, 21, 8040. https://doi.org/10.3390/ijms21218040
Honsho M, Abe Y, Imoto Y, Chang Z-F, Mandel H, Falik-Zaccai TC, Fujiki Y. Mammalian Homologue NME3 of DYNAMO1 Regulates Peroxisome Division. International Journal of Molecular Sciences. 2020; 21(21):8040. https://doi.org/10.3390/ijms21218040
Chicago/Turabian StyleHonsho, Masanori, Yuichi Abe, Yuuta Imoto, Zee-Fen Chang, Hanna Mandel, Tzipora C. Falik-Zaccai, and Yukio Fujiki. 2020. "Mammalian Homologue NME3 of DYNAMO1 Regulates Peroxisome Division" International Journal of Molecular Sciences 21, no. 21: 8040. https://doi.org/10.3390/ijms21218040