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Article

The Impact of Drosophila Awd/NME1/2 Levels on Notch and Wg Signaling Pathways

by
Giulia Serafini
1,2,
Giorgia Giordani
1,
Luca Grillini
1,
Davide Andrenacci
3,4,
Giuseppe Gargiulo
1,* and
Valeria Cavaliere
1,*
1
Dipartimento di Farmacia e Biotecnologie, Alma Mater Studiorum Università di Bologna, 40126 Bologna, Italy
2
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
3
Unit of Bologna, CNR Institute of Molecular Genetics “Luigi-Luca Cavalli-Sforza”, 40136 Bologna, Italy
4
IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(19), 7257; https://doi.org/10.3390/ijms21197257
Submission received: 12 August 2020 / Revised: 18 September 2020 / Accepted: 29 September 2020 / Published: 1 October 2020
(This article belongs to the Special Issue Selected Papers from the 11th International Conference on the NME/NDPK/NM23/AWD Gene Family (NME 2019))
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Abstract

:
Awd, the Drosophila homologue of NME1/2 metastasis suppressors, plays key roles in many signaling pathways. Mosaic analysis of the null awdJ2A4 allele showed that loss of awd gene function blocks Notch signaling and the expression of its target genes including the Wingless (Wg/Wnt1) morphogen. We also showed that RNA interference (RNAi)-mediated awd silencing (awdi) in larval wing disc leads to chromosomal instability (CIN) and to Jun amino-terminal kinases (JNK)-mediated cell death. Here we show that this cell death is independent of p53 activity. Based on our previous finding showing that forced survival of awdi-CIN cells leads to aneuploidy without the hyperproliferative effect, we investigated the Wg expression in awdi wing disc cells. Interestingly, the Wg protein is expressed in its correct dorso-ventral domain but shows an altered cellular distribution which impairs its signaling. Further, we show that RNAi-mediated knock down of awd in wing discs does not affect Notch signaling. Thus, our analysis of the hypomorphic phenotype arising from awd downregulation uncovers a dose-dependent effect of Awd in Notch and Wg signaling.

1. Introduction

The Drosophila abnormal wing disc (awd) gene is the only homolog of NME1/2 genes identified to date [1,2]. The NME1 and NME2 genes belong to a family of ten related genes in humans that are evolutionarily conserved [3]. The NME1 gene, the first identified metastasis suppressor gene [2], and the NME2 gene are the ones most often implicated in tumor progression. The NME proteins have several biochemical functions and their tumorigenic role is far to be completely understood. Metastasis suppression was found in a variety of tumor types including melanoma, breast, ovarian, colon and hepatocellular carcinomas [4]. In these malignancies, NME expression is inversely correlated with poor survival and tumor grade. On the contrary, in neuroblastoma and hematological malignancies, NME expression is indicative of a poor patient prognosis [5,6]. Moreover, extracellular NME proteins have been detected in the medium of cell lines and in human body fluids where their levels vary in different pathological conditions [7]. The physiological function of the secreted NME proteins is still a matter of debate.
Genetic studies on Awd confirmed the multifunctional nature of this protein that fulfils numerous molecular and cellular functions [8]. Awd endocytic function in different tissues is crucial in modulating internalization of signaling receptors and in intracellular trafficking of Notch [9,10,11]. Consistent with the high degree of functional conservation between Awd and its mammalian counterparts, recent studies have shown a role for the NME1/2 proteins in vesicular transport [12]. NME1 is known to inhibit the migratory and invasive potential of cancer cells. It has been shown that the endocytic function of Awd and its human and C. elegans homologues is involved in the motility of migrating cells. A dosage-dependent effect of Awd/NDK-1 (Nucleoside Diphosphate Kinase-1) has been reported on the migration of tracheal and border cells in Drosophila and of distal tip cells in C. elegans [13]. Furthermore, a role in the maintenance of genomic stability has also been assigned to both NME1 and Awd [14,15]. In our previous analyses we found that in larval wing disc mitotic clones lacking the Awd function Notch signaling was halted, and the expression of its target genes, including the signaling molecule Wg, was blocked [10,16]. RNA interference (RNAi)-mediated awd (awdi) gene silencing in the wing disc posterior compartment allowed us to uncover the awd role in maintenance of genomic stability [15]. RNAi-mediated awd silencing led to CIN (Chromosomal Instability) and to JNK (Jun amino-terminal kinases)-mediated cell death of larval wing disc cells. Furthermore, block of apoptosis of awdi CIN cells resulted in upregulation of MMP1 (Matrix Metalloproteinase 1), cell movement toward the basal side of the epithelium, and aneuploidy. The forced survival of awdi CIN cells did not lead to wing disc hyperplasia, differently from the overgrowth arising from forced survival of CIN cells lacking the function of spindle assembly checkpoint (SAC) genes [15,17].
Unrepaired DNA damage is deemed as a contributor to major genome rearrangements including aneuploidy. Double-strand DNA breaks (DSBs) are among the most severe DNA lesions that lead to the cellular DNA damage response (DDR) to repair genomic lesions. The DDR activates the p53 tumor suppressor that in turn promotes the expression of proteins involved in multiple processes including DNA repair and apoptosis [18].
Herein, we further study the cellular effects induced by awd silencing. We find that the block of p53 activity in awdi wing disc cells does not enhance apoptotic cell death suggesting that awdi does not induce the p53-mediated response to repair DNA damage. The results of our analyses also show that, in contrast with awd where complete loss of function that halts Notch signaling and blocks Wg expression, awdi does not perturb Notch signaling but alters Wg distribution and signaling fully accounting for the lack of wing disc overgrowth. Thus, the present analysis of the hypomorphic phenotype arising from awd downregulation uncovers a dose-dependent effect of Awd in Notch and Wg signaling.

2. Results

2.1. Effects of Awd Silencing in the Larval Wing Disc

The Drosophila larval development is characterized by the progression of timed developmental stages. After larval hatching, larvae go through three instar stages separated by moults, followed by pupariation and metamorphosis, giving rise to the adults. We analyzed the effect of awd downregulation in the wing disc, the primordium of Drosophila adult wing, which is a powerful model for studying tumorigenic growth [19] (Figure 1A). The wing disc is composed of undifferentiated cells that divide throughout larval development before undergoing metamorphosis during pupal stage to form the wing and part of the thoracic wall of the adult fly.
We applied the UAS/Gal4 system to downregulate in vivo awd gene expression by transgene-mediated RNA interference (RNAi) [20] (Figure 1B). To mark cells with downregulated Awd level we coexpressed the UAS-awd interfering transgene (awdi) and the GFP marker. The engrailed-Gal4 (en-Gal4) enhancer trap line has been used to control expression of both transgenes. This line expresses the Gal4 transcriptional activator within the posterior compartment of developing tissues [21]. Using the en-Gal4 driver we downregulated Awd expression throughout development and analyzed the cellular phenotype arising from Awd silencing in the wing disc. Given the posterior compartment specificity of the en-Gal4 driver, in each wing disc from en-Gal4, UAS-GFP; UAS-awdi (hereafter abbreviated en>awdi) larvae, the posterior domain will be composed by cells undergoing Awd downregulation (GFP-positive) while the adjacent anterior domain containing normal cells will serve as control (GFP-negative) (Figure 1A,B). Successful knockdown of Awd was observed as seen by the reduced Awd protein level in the posterior compartment of en>awdi wing disc (Figure 1C,D). Consistent with this data, a significant decrease of awd transcript (Figure 1E) was detected by quantitative RT-PCR (qRT-PCR) analysis of wing disc and cephalic complexes expressing awdi in the posterior compartment under control of en-Gal4.
We have previously reported that awd silencing in the posterior domain induces CIN without hyperproliferative effect [15]. In the present study, we quantified the ratio between the posterior domain area and the total area of both en>awdi wing discs (n = 10) and control en>+ wing discs (n = 10), marked by GFP expression. The results showed a statistically significant reduction (Figure 2E) of the posterior domain of en>awdi wing disc (Figure 2C,D) in comparison with the same domain of control wing disc (Figure 2A,B). We extended our analysis to the wing of en>awdi adults which shows defects in the posterior region [15]. The quantification of the ratio between the posterior domain area and total wing area showed a statistically significant reduction of the posterior domain (Figure 2F–H).

2.2. awdi Induced Cell Death is p53 Independent

p53 activity is necessary to repair IR-induced DSBs in wing disc [22]. Furthermore, IR-induced DSBs lead to JNK-mediated apoptotic cell death [23]. It has also been reported that chromosomal instability leads to p53-independent cell death [17,24]. Therefore, we analyzed apoptosis in en>awdi wing disc lacking p53 activity. We coexpressed awdi and the transgene coding for the H159N dominant negative form of p53 (p53DN) [25] in the posterior domain of wing disc. Then, we compared apoptosis in en>awdi and en>p53DN, awdi by looking at Casp3 activation. Up to 65% of en>awdi wing discs (n = 23) showed activated-Caspase 3 signal in the posterior domain, confirming our previous results [15] (Figure 3A–C,G). The coexpression of awdi and p53DN transgenes resulted in 77% wing discs (n = 26) with activated-Caspase 3 in the posterior domain (Figure 3D–F,G). Thus, block of the p53 function does not modify the occurrence of apoptosis in en>awdi wing discs showing that awdi leads to p53-independent cell death. This result further suggests that awdi does not trigger the p53 activity of DSB repair.

2.3. Awd Gene Function is Required for Wg/Wnt Signaling

Overexpression of the Wg mitogen in aneuploid CIN cells induces over proliferation of surrounding cells in the wing disc [17]. The absence of hyperproliferative effect in tissue depleted of Awd function is consistent with our previous findings obtained using the null awdJ2A4 allele, in which the Notch signaling is impaired and blocks Wg expression at the wing disc DV boundary [10]. To monitor Notch signaling we used reporter constructs carrying either the lacZ or GFP reporter genes under control of regulatory elements from Notch-responsive genes (see Section 4.1). The expression of the GBE-lacZ (GBE) reporter for Notch activity [26] is absent in clones of cells homozygous for the awdJ2A4 allele (Figure 4A–C) [10]. Since RNAi often leads to incomplete loss of gene function [27], it seemed interesting to analyze the Notch and Wg signaling pathways in en>awdi discs. The expression of the NRE-eGFP transcriptional reporter for Notch activity [28] is unaltered at the dorso-ventral boundary of the wing disc showing that the Notch signaling is active in the posterior compartment of all the en>awdi wing discs (n = 24). (Figure 4D–F).
Consistent with this result, we observed that the Wg protein is expressed in the DV boundary of anterior and posterior compartments of en>awdi wing discs but in these cells the Wg protein had an altered distribution (Figure 5A–C). The comparison between anterior and posterior Wg-producing cells clearly showed a marked accumulation in large aggregates of the Wg protein in the latter (Figure 5B,C, arrowheads). Besides Wg-producing cells, the Wg protein is detected also in Wg-receiving cells where it forms spots whose intensity and number decrease with distance from the Wg-producing cells [29]. This punctate staining is well evident in the posterior compartment of en>awdi disc showing Wg secretion and gradient formation (Figure 5C, empty arrowheads). In order to assess Wg activity, we analyzed the expression of senseless (sens), a Wg short-range target gene [30]. In wild type wing discs Sens is expressed in two stripes of signal-receiving cells flanking the Wg-expressing cells at the DV boundary. In the posterior domain of en>awdi wing disc, the expression of sens in signal-receiving cells abutting Wg-expressing cells is lost (Figure 5D,F) indicating the loss of Wg short-range signaling. Unexpectedly, ectopic Sens expression is detected in the posterior domain far from DV Wg-producing cells.
These results indicate that RNAi-mediated awd silencing driven by en-Gal4 only partially suppresses awd gene function leading to a hypomorphic phenotype. Indeed, 23.48% of en>awdi survived to adult stage (n = 132). To further confirm the hypomorphic effect of en>awdi we compared the ubiquitous awd silencing phenotype to the one of homozygous null awdJ2A4 mutant. The awdJ2A4 mutation is lethal recessive [31]. We found that ubiquitous silencing of awd driven by the tub-Gal4 also caused lethality of tub>awdi flies. Wild type larval development proceeds through two different stages before reaching the third instar stage at about 120 h after egg laying at 25 °C. During late third instar the larvae start crawling and pupariate. To examine larval viability, we transferred third instar larvae onto fresh food and waited for them to complete development. While all awdJ2A4 null larvae failed to pupariate and die (n = 120), 64.9% of tub>awdi larvae (n = 111) reached the pupal stage. Thus, the ubiquitous awd silencing allows development to proceed further confirming that RNAi mediated awd silencing results in a reduction of awd level.

3. Discussion

In the present study, we have analyzed the effect of awd downregulation driven by RNAi in the wing imaginal disc model. We have already shown that reduction of awd function in the posterior compartment of wing disc causes genomic instability and that forced survival of awdi CIN cells does not lead to overgrowth of the wing disc [15]. Our present data indicate that reduced awd gene expression results in a hypomorphic phenotype that leads to p53-independent cell death. Furthermore, we show that awdi halts the Wg signaling pathway in a wing disc fully accounting for the lack of wing disc overgrowth. In contrast with awd complete loss of function that affects Notch signaling and blocks Wg expression, the awdi hypomorph shows unperturbed Notch signaling and altered distribution of Wg protein at the DV boundary of wing disc. Thus, the study of awdi hypomorphic effect advanced our knowledge of Awd function indicating for the first time that Awd is involved in Wg signaling.
Awd has a well-known endocytic function that is required in multiple tissues for many growth-factor-mediated signaling pathways [8]. Wg distribution in awdi cells at the DV boundary of the wing disc clearly indicates altered Wg trafficking that blocks expression of the sens short-range target gene.
The Wg morphogen acts at short- and long-range distances thanks to different transport mechanisms. The molecular events underlying Wg secretion are partially known but a key role emerged for endocytosis in both short and long range Wg signaling.
Short-range signaling mainly occurs via apical secretion of Wg from the Wg-producing cell [32]. Evenness interrupted/Wintless (Evi/Wls) and p24/Opium chaperonins are involved in the ER to PM transport of Wg [33,34]. After releasing Wg in the extracellular space, Evi/Wls is endocytosed by the Wg-producing cell and undergoes a retromer-dependent retrograde transport to the trans Golgi network (TGN) [34,35]. Loss of function of the retromer component Vps35 in the wing disc results in reduced Wg secretion and in loss of expression of the short-range target sens while it leaves unaltered the expression of the long-range target distalless [36]. In summary, endocytosis of Evi/Wls chaperonin, and subsequent retrograde transport to TGN, is a key step in short-range Wg signaling. Therefore, we suggest that the Awd endocytic function could be involved in recycling back the Evi chaperonin to the Wg-producing cell (Figure 6).
Endocytosis in Wg-producing cells also plays an additional role since loss of shi/dyn function results in accumulation of Wg protein in Wg-producing cells at the DV boundary of wing discs [29]. Further analysis of Wg trafficking showed that Shi/Dyn-mediated endocytosis from the apical surface is a key step in Wg transcytosis [37]. Wg is secreted from the apical surface where it activates short-range signaling. After Shi/Dyn-mediated endocytosis, Wg undergoes the endosomal route and is transported to the basolateral membrane to be secreted and form the extracellular gradient that activates long-range target genes.
Our analysis of Wg protein distribution in awdi wing disc cells showed its accumulation in cells at the DV boundary and the lack of its short-range signaling as shown by the loss of sens expression in cells flanking the Wg-expressing cells. The presence of spots in neighboring cells indicates that the Wg protein travels at a distance from the DV boundary and forms its gradient. We suggest that this fraction of Wg protein could be responsible for the ectopic expression of Sens detected in the posterior compartment of the en>awdi wing disc. This is an interesting finding, which warrants further investigation.
In addition, Wnt signaling is involved in the regulation of microtubule stability and orientation of the mitotic spindle [38]. The Wnt pathway component β-catenin and its interacting proteins localize at the centrosome and their function is required for mitotic progression. Recently, work in Drosophila neurons showed that Wnt signaling proteins play a key role in controlling polarity and nucleation of dendritic microtubules [39]. Since these Wnt signaling proteins colocalize with Rab5, it has been suggested that they are located on early endosomes. An interesting possibility could be that impaired Wg signaling contributes to the aneuploidy arising from awdi.
Our present data showed that absence of Awd and its reduced levels exert an opposing effect on Notch signaling (Figure 6). In a previous work we have already shown Awd requirement in the early step of Notch intracellular trafficking, after the Shi/Dyn-mediated cleavage of membrane invagination, for Rab5 activity in early endosome maturation [10]. Rab5 regulates early steps of endocytosis including endosomal sorting and fusion of endocytic vesicles. Furthermore, Rab5 plays a key role in the assembly of endosomal machinery since its depletion below a critical level leads to the loss of the endolysosomal pathway [40]. Therefore, we suggest that an absent and reduced Awd level impacts different trafficking pathways dependent on Rab5 activity leading to an opposite effect on Notch signaling.
awd is the only NME1/2 homolog gene identified in Drosophila. NME1/2 are metastasis suppressor genes that exhibit oncogenic function in some tumoral context. Furthermore, NME1/2 expression levels are inversely correlated with patient’s prognosis in different cancer cohorts [8,41]. Interestingly, our data show that different levels of Awd elicit different effect on key signal transduction pathways such as Notch and Wg. Moreover, Wg signaling requires different transport mechanisms that are context- and tissue-specific [42]. Further studies of the cellular effects arising from awd downregulation could help in understanding the complex functions played by NME1/2 proteins.

4. Materials and Methods

4.1. Drosophila Stocks

Drosophila stocks were maintained on standard cornmeal/yeast medium under 12:12 h light/dark cycle at 25 °C and crosses were carried out at the same temperature. The following stocks were obtained from Bloomington Drosophila Stock Center: #33712: y; UAS-awd-RNAi/TM3, Sb; #8420: y,w; UAS-p53H159N; #5072: w; UAS-p35; #30564: y,w, en-Gal4e16E (en-Gal4). The w, FRT82B, awdJ2A4/TM6B was from T. Hsu, (National Central University, Zhongli, Taiwan) and en-Gal4, UAS-GFPmCD8/Gla, Bc was from D. Grifoni (University of Bologna, Bologna, Italy). Notch signaling was monitored using the Gbe+Su(H)m8-lacZ from S. Bray (University of Cambridge, Cambridge, UK) [26] and the NRE-eGFP #30727 from BDSC. Activation of the Notch pathway in the developing Drosophila wing leads to the Su(H) (Suppressor of Hairless)-dependent expression of target genes like E(spl)m8 (Enhancer of Split m8). GBE and NRE reporters consist of Su(H) binding motifs which control reporter gene expression and represent a readout for Notch pathway activity.
Larvae of the following genotypes (1) en-Gal4, UAS-GFPmCD8/+; UAS-awdRNAi/+ (2) en-Gal4, UAS-GFPmCD8/UAS-p53H159N; UAS-awdRNAi/+ (3) en-Gal4, UAS-GFPmCD8/+ were obtained by crossing the parental strains. To induce awdJ2A4 MARCM clones in wing discs, progeny from appropriate crosses was collected for 24 h. At 48 h after egg laying progeny was subjected to 1-hour heat shock treatment at 37 °C and kept at 25 °C to allow further development. yw, hs-flp/Gbe+Su(H)m8lacZ; act-Gal4, UAS-GFP/+; FRT82B, awdJ2A4/FRT82B, act-Gal80 third instar stage larvae were then collected and subjected to immunohistochemistry experiments. The genotypes of flies and larvae used for the analyses are the following: Figure 1C,D, Figure 2C,D, Figure 3A–C, Figure 5A–F: en-Gal4, UAS-GFPmCD8/+; UAS-awdi/+. Figure 2A,B: en-Gal4, UAS-GFPmCD8/UAS-nGFP. Figure 3D–F: en-Gal4, UAS-GFPmCD8/p53DN; UAS-awdi/+. Figure 4A–C: hs-flp/Gbe+Su(H)m8lacZ; act-Gal4, UAS-GFP/+; FRT82B, awdJ2A4/FRT82B, act-Gal80. Figure 4D–F en-gal4/NRE-eGFP; UAS-awdi/+.

4.2. Immunofluorescence Microscopy

Larval tissues were collected and treated at 110–120 h after egg laying. Larvae were dissected in 1xPBS at room temperature and fixed for 20 min in 4% formaldehyde and the immunostaining procedure was performed as previously described [43]. The following primary antibodies were used: rabbit anti-Awd (1:2000; [9]); rabbit anti-cleaved-Caspase3 (1:100; Cell Signaling Technology); mouse monoclonal anti-β-gal (1:25; 40-1A, DSHB); mouse monoclonal anti-Wg antibody (1:50; 4D4, DSHB); guinea pig anti-Sens (1:1000; [30]). The following secondary antibodies from Jackson ImmunoResearch Laboratories were used: Cy3-conjugated goat anti-mouse (1:500); Cy3- (1:1000) or DyLight 649- conjugated anti-rabbit IgG (1:500) and Cy3-conjugated guinea pig (1:800). DNA staining was carried out by incubating wing discs for 10 min with 4′,6-diamidino- 2-phenylindole (DAPI; Sigma-Merck) at 0.5 μg/mL in PBS followed by several washes with PBS. The samples were then mounted in Fluoromount-G (Electron Microscopy Sciences, Hatfield, PA, USA) and were subsequently analyzed with conventional epifluorescence on a Nikon Eclipse 90i microscope (Nikon, Japan) and with a TCS SL Leica confocal system (Leica, Germany). Digital images were processed and assembled using Adobe Photoshop software (Adobe, San Jose, CA, USA). No biased image manipulations were applied.

4.3. Total RNA Extraction, cDNA Synthesis and Real Time PCR

Ten cephalic complexes which include the brain hemispheres and ventral nerve cord, and the associated imaginal discs including the wing discs were dissected from en>awdi and en>+ control third instar larvae in PBS. Three independent biological replicates were analyzed. Total RNA was extracted using TRI Reagent (Sigma-Merck, Darmstadt, Germany) RNA extraction protocol as described in [44,45]. RNA concentration and purity were estimated by measuring the absorbance at 260 nm and A260/A280 ratio. 250 ng RNA was retro-transcribed with Omniscript RT Kit (Qiagen, Hilden, Germany) following the manufacturer’s protocol in an MJ Research PTC-100 Programmable Thermal Controller (BioRad, Hercules, CA, USA). Relative abundance of awd transcripts was determined by real-time PCR using SsoFast EvaGreen Supermix (BioRad, Hercules, CA, USA) according to the manufacturer’s protocol. Real-time RT–PCR was performed in the CFX Connect Real-Time PCR Detection System (BioRad, Hercules, CA, USA) through the BioRad Manager Software Version 3.1. All transcript expression values were normalized to Rpl32. Primers used for Rpl32 transcript were 5′GACGCTTCAAGGGACAGTATCTG3′ and 5′AAACGCGGTTCTGCATGAG3′; primers used for awd transcript were 5′TGGTCGCCCTGAAGTTCAC3′ and 5′TGACCACATTCAGACCCTCC3′. Relative quantification followed the 2−ΔΔct method [46].

4.4. Measure of Posterior Compartment

The measures of the posterior compartment of wing discs were performed on en>+ and en>awdi larval wing discs dissected and fixed as previously described. The en>+ and en>awdi wings were dissected from adult males, dehydrated in ethanol 100% and then mounted on microscope slides in lactic acid/ethanol (6:5). Images of wing discs and adult wings were captured using a Nikon Eclipse 90i microscope and acquired with a Nikon Digital Sight camera (Nikon, Japan).
For each wing disc the total area (DAPI) and the area occupied by GFP+ cells (en-Gal4) were calculated using ImageJ software (National Institutes of Health, Bethesda, MD, USA). Likewise, the total area and the posterior area of the adult wing were defined following conventional morphology using the same software. The ratio between the area of the posterior domain and the total area was calculated.

4.5. Statistical Analyses

GraphPad Prism 6 software was used for statistical analysis. Statistical significance was determined based on the unpaired one-tail t-test performed on three independent experiments. p < 0.05 was considered statistically significant (* = p < 0.05; ** = p < 0.01. *** = p <0.001 and **** = p < 0.001). All results are expressed as the mean ± standard deviation (SD).

Author Contributions

V.C. and G.G. (Giuseppe Gargiulo) designed and supervised the study. G.S., L.G. and G.G. (Giorgia Giordani) performed the experiments. V.C., D.A., G.G. (Giuseppe Gargiulo) and G.G. (Giorgia Giordani) analyzed and interpreted the data. V.C. and G.G. (Giuseppe Gargiulo) wrote the manuscript with input from D.A. and G.G. (Giorgia Giordani). All authors have read and agreed to the published version of the manuscript.

Funding

Authors gratefully acknowledge funding from the Association for International Cancer Research, AICR-WWCR grant ref. 11-0738, to V. Cavaliere and from the University of Bologna 2019 to G. Gargiulo and V. Cavaliere.

Acknowledgments

We thank our friend and colleague T. Hsu for our long-lasting collaboration work on Awd. We thank S. Bray, H. Bellen, R. Bernardoni and D. Grifoni for sharing fly stocks and antibodies. We acknowledge the Bloomington Drosophila Stock Center and the Developmental Studies Hybridoma Bank (DSHB). We also thank P. Romani, M. Ignesti and E. Mezzofanti for their early contribution to the awd project. A special thank goes to Marco Privitera for his graphic work.

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

awdiRNA interference (RNAi)-mediated awd silencing
CINChromosomal Instability
DDRDNA Damage Response
DSBsDouble-Strand DNA Breaks
DVDorso-Ventral
EREndoplasmic Reticulum
JNKJun amino-terminal kinases
MMP1Matrix Metalloproteinase 1
NDK-1Nucleoside Diphosphate Kinase-1
SACSpindle Assembly Checkpoint
TGNTrans Golgi Network

References

  1. Rosengard, A.M.; Krutzsch, H.C.; Shearn, A.; Biggs, J.R.; Barker, E.; Margulies, I.M.; King, C.R.; Liotta, L.A.; Steeg, P.S. Reduced Nm23/Awd protein in tumour metastasis and aberrant Drosophila development. Nature 1989, 342, 177–180. [Google Scholar] [CrossRef]
  2. Steeg, P.S.; Bevilacqua, G.; Kopper, L.; Thorgeirsson, U.P.; Talmadge, J.E.; Liotta, L.A.; Sobel, M.E. Evidence for a novel gene associated with low tumor metastatic potential. J. Natl. Cancer Inst. 1988, 80, 200–204. [Google Scholar] [CrossRef]
  3. Desvignes, T.; Pontarotti, P.; Fauvel, C.; Bobe, J. Nme protein family evolutionary history, a vertebrate perspective. BMC Evol. Biol. 2009, 9, 256. [Google Scholar] [CrossRef] [Green Version]
  4. Hartsough, M.T.; Steeg, P.S. Nm23/nucleoside diphosphate kinase in human cancers. J. Bioenerg. Biomembr. 2000, 32, 301–308. [Google Scholar] [CrossRef]
  5. Leone, A.; Seeger, R.C.; Hong, C.M.; Hu, Y.Y.; Arboleda, M.J.; Brodeur, G.M.; Stram, D.; Slamon, D.J.; Steeg, P.S. Evidence for nm23 RNA overexpression, DNA amplification and mutation in aggressive childhood neuroblastomas. Oncogene 1993, 8, 855–865. [Google Scholar] [PubMed]
  6. Niitsu, N.; Okabe-Kado, J.; Okamoto, M.; Takagi, T.; Yoshida, T.; Aoki, S.; Hirano, M.; Honma, Y. Serum nm23-H1 protein as a prognostic factor in aggressive non-Hodgkin lymphoma. Blood J. Am. Soc. Hematol. 2001, 97, 1202–1210. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  7. Romani, P.; Ignesti, M.; Gargiulo, G.; Hsu, T.; Cavaliere, V. Extracellular NME proteins: A player or a bystander? Lab. Investig. 2018, 98, 248–257. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  8. Nallamothu, G.; Dammai, V.; Hsu, T. Developmental function of Nm23/awd: A mediator of endocytosis. Mol. Cell. Biochem. 2009, 329, 35–44. [Google Scholar] [CrossRef] [Green Version]
  9. Dammai, V.; Adryan, B.; Lavenburg, K.R.; Hsu, T. Drosophila awd, the homolog of human nm23, regulates FGF receptor levels and functions synergistically with shi/dynamin during tracheal development. Genes Dev. 2003, 17, 2812–2824. [Google Scholar] [CrossRef] [Green Version]
  10. Ignesti, M.; Barraco, M.; Nallamothu, G.; Woolworth, J.A.; Duchi, S.; Gargiulo, G.; Cavaliere, V.; Hsu, T. Notch signaling during development requires the function of awd, the Drosophila homolog of human metastasis suppressor gene Nm23. BMC Biol. 2014, 12, 12. [Google Scholar] [CrossRef] [Green Version]
  11. Nallamothu, G.; Woolworth, J.A.; Dammai, V.; Hsu, T. awd, the homolog of metastasis suppressor gene Nm23, regulates Drosophila epithelial cell invasion. Mol. Cell. Biol. 2008, 28, 1964–1973. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  12. Khan, I.; Gril, B.; Steeg, P.S. Metastasis Suppressors NME1 and NME2 Promote Dynamin 2 Oligomerization and Regulate Tumor Cell Endocytosis, Motility, and Metastasis. Cancer Res. 2019, 79, 4689–4702. [Google Scholar] [CrossRef] [PubMed]
  13. Mátyási, B.; Farkas, Z.; Kopper, L.; Sebestyén, A.; Boissan, M.; Mehta, A.; Takács-Vellai, K. The Function of NM23-H1/NME1 and Its Homologs in Major Processes Linked to Metastasis. Pathol. Oncol. Res. 2020, 26, 49–61. [Google Scholar] [CrossRef] [Green Version]
  14. Conery, A.R.; Sever, S.; Harlow, E. Nucleoside diphosphate kinase Nm23-H1 regulates chromosomal stability by activating the GTPase dynamin during cytokinesis. Proc. Natl. Acad. Sci. USA 2010, 107, 15461–15466. [Google Scholar] [CrossRef] [Green Version]
  15. Romani, P.; Duchi, S.; Gargiulo, G.; Cavaliere, V. Evidence for a novel function of Awd in maintenance of genomic stability. Sci. Rep. 2017, 7, 16820. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. De Celis, J.F.; Garcia-Bellido, A.; Bray, S.J. Activation and function of Notch at the dorsal-ventral boundary of the wing imaginal disc. Development 1996, 122, 359–369. [Google Scholar]
  17. Dekanty, A.; Barrio, L.; Muzzopappa, M.; Auer, H.; Milan, M. Aneuploidy-induced delaminating cells drive tumorigenesis in Drosophila epithelia. Proc. Natl. Acad. Sci. USA 2012, 109, 20549–20554. [Google Scholar] [CrossRef] [Green Version]
  18. Brodsky, M.H.; Weinert, B.T.; Tsang, G.; Rong, Y.S.; McGinnis, N.M.; Golic, K.G.; Rio, D.C.; Rubin, G.M. Drosophila melanogaster MNK/Chk2 and p53 regulate multiple DNA repair and apoptotic pathways following DNA damage. Mol. Cell. Biol. 2004, 24, 1219–1231. [Google Scholar] [CrossRef] [Green Version]
  19. Pagliarini, R.A.; Xu, T. A genetic screen in Drosophila for metastatic behavior. Science 2003, 302, 1227–1231. [Google Scholar] [CrossRef]
  20. Brand, A.H.; Perrimon, N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 1993, 118, 401–415. [Google Scholar]
  21. Tabata, T.; Schwartz, C.; Gustavson, E.; Ali, Z.; Kornberg, T.B. Creating a Drosophila wing de novo, the role of engrailed, and the compartment border hypothesis. Development 1995, 121, 3359–3369. [Google Scholar] [PubMed]
  22. Wells, B.S.; Johnston, L.A. Maintenance of imaginal disc plasticity and regenerative potential in Drosophila by p53. Dev. Biol. 2012, 361, 263–276. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  23. Dekanty, A.; Barrio, L.; Milan, M. Contributions of DNA repair, cell cycle checkpoints and cell death to suppressing the DNA damage-induced tumorigenic behavior of Drosophila epithelial cells. Oncogene 2015, 34, 978–985. [Google Scholar] [CrossRef] [PubMed]
  24. Dekanty, A.; Milán, M. Aneuploidy, cell delamination and tumorigenesis in Drosophila epithelia. Cell Cycle 2013, 12, 728–731. [Google Scholar] [CrossRef] [Green Version]
  25. Ollmann, M.; Young, L.M.; Di Como, C.J.; Karim, F.; Belvin, M.; Robertson, S.; Whittaker, K.; Demsky, M.; Fisher, W.W.; Buchman, A.; et al. Drosophila p53 is a structural and functional homolog of the tumor suppressor p53. Cell 2000, 101, 91–101. [Google Scholar] [CrossRef] [Green Version]
  26. Furriols, M.; Bray, S. A model Notch response element detects Suppressor of Hairless-dependent molecular switch. Curr. Biol. 2001, 11, 60–64. [Google Scholar] [CrossRef] [Green Version]
  27. Perkins, L.A.; Holderbaum, L.; Tao, R.; Hu, Y.; Sopko, R.; McCall, K.; Yang-Zhou, D.; Flockhart, I.; Binari, R.; Shim, H.S.; et al. The Transgenic RNAi Project at Harvard Medical School: Resources and Validation. Genetics 2015, 201, 843–852. [Google Scholar] [CrossRef]
  28. Saj, A.; Arziman, Z.; Stempfle, D.; van Belle, W.; Sauder, U.; Horn, T.; Durrenberger, M.; Paro, R.; Boutros, M.; Merdes, G. A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. Dev. Cell 2010, 18, 862–876. [Google Scholar] [CrossRef] [Green Version]
  29. Strigini, M.; Cohen, S.M. Wingless gradient formation in the Drosophila wing. Curr. Biol. 2000, 10, 293–300. [Google Scholar] [CrossRef] [Green Version]
  30. Nolo, R.; Abbott, L.A.; Bellen, H.J. Senseless, a Zn finger transcription factor, is necessary and sufficient for sensory organ development in Drosophila. Cell 2000, 102, 349–362. [Google Scholar] [CrossRef] [Green Version]
  31. Krishnan, K.S.; Rikhy, R.; Rao, S.; Shivalkar, M.; Mosko, M.; Narayanan, R.; Etter, P.; Estes, P.S.; Ramaswami, M. Nucleoside diphosphate kinase, a source of GTP, is required for dynamin-dependent synaptic vesicle recycling. Neuron 2001, 30, 197–210. [Google Scholar] [CrossRef] [Green Version]
  32. Bartscherer, K.; Pelte, N.; Ingelfinger, D.; Boutros, M. Secretion of Wnt ligands requires Evi, a conserved transmembrane protein. Cell 2006, 125, 523–533. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  33. Buechling, T.; Chaudhary, V.; Spirohn, K.; Weiss, M.; Boutros, M. p24 proteins are required for secretion of Wnt ligands. EMBO Rep. 2011, 12, 1265–1272. [Google Scholar] [CrossRef] [PubMed]
  34. Yu, J.; Chia, J.; Canning, C.A.; Jones, C.M.; Bard, F.A.; Virshup, D.M. WLS retrograde transport to the endoplasmic reticulum during Wnt secretion. Dev. Cell 2014, 29, 277–291. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Belenkaya, T.Y.; Wu, Y.; Tang, X.; Zhou, B.; Cheng, L.; Sharma, Y.V.; Yan, D.; Selva, E.M.; Lin, X. The retromer complex influences Wnt secretion by recycling wntless from endosomes to the trans-Golgi network. Dev. Cell 2008, 14, 120–131. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  36. Franch-Marro, X.; Wendler, F.; Guidato, S.; Griffith, J.; Baena-Lopez, A.; Itasaki, N.; Maurice, M.M.; Vincent, J.P. Wingless secretion requires endosome-to-Golgi retrieval of Wntless/Evi/Sprinter by the retromer complex. Nat. Cell Biol. 2008, 10, 170–177. [Google Scholar] [CrossRef]
  37. Yamazaki, Y.; Palmer, L.; Alexandre, C.; Kakugawa, S.; Beckett, K.; Gaugue, I.; Palmer, R.H.; Vincent, J.P. Godzilla-dependent transcytosis promotes Wingless signalling in Drosophila wing imaginal discs. Nat. Cell Biol. 2016, 18, 451–457. [Google Scholar] [CrossRef] [Green Version]
  38. Mbom, B.C.; Nelson, W.J.; Barth, A. beta-catenin at the centrosome: Discrete pools of beta-catenin communicate during mitosis and may co-ordinate centrosome functions and cell cycle progression. Bioessays 2013, 35, 804–809. [Google Scholar] [CrossRef] [Green Version]
  39. Weiner, A.T.; Seebold, D.Y.; Torres-Gutierrez, P.; Folker, C.; Swope, R.D.; Kothe, G.O.; Stoltz, J.G.; Zalenski, M.K.; Kozlowski, C.; Barbera, D.J.; et al. Endosomal Wnt signaling proteins control microtubule nucleation in dendrites. PLoS Biol. 2020, 18, e3000647. [Google Scholar] [CrossRef] [Green Version]
  40. Zeigerer, A.; Gilleron, J.; Bogorad, R.L.; Marsico, G.; Nonaka, H.; Seifert, S.; Epstein-Barash, H.; Kuchimanchi, S.; Peng, C.G.; Ruda, V.M.; et al. Rab5 is necessary for the biogenesis of the endolysosomal system in vivo. Nature 2012, 485, 465–470. [Google Scholar] [CrossRef]
  41. Hsu, T.; Steeg, P.S.; Zollo, M.; Wieland, T. Progress on Nme (NDP kinase/Nm23/Awd) gene family-related functions derived from animal model systems: Studies on development, cardiovascular disease, and cancer metastasis exemplified. Naunyn Schmiedebergs Arch. Pharmacol. 2015, 388, 109–117. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  42. Routledge, D.; Scholpp, S. Mechanisms of intercellular Wnt transport. Development 2019, 146. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Mezzofanti, E.; Ignesti, M.; Hsu, T.; Gargiulo, G.; Cavaliere, V. Vps28 Is Involved in the Intracellular Trafficking of Awd, the Drosophila Homolog of NME1/2. Front. Physiol. 2019, 10, 983. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  44. Cavaliere, V.; Lattanzi, G.; Andrenacci, D. Silencing of Euchromatic Transposable Elements as a Consequence of Nuclear Lamina Dysfunction. Cells 2020, 9, 625. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  45. Ignesti, M.; Andrenacci, D.; Fischer, B.; Cavaliere, V.; Gargiulo, G. Comparative Expression Profiling of Wild Type Drosophila Malpighian Tubules and von Hippel-Lindau Haploinsufficient Mutant. Front. Physiol. 2019, 10, 619. [Google Scholar] [CrossRef]
  46. Livak, K.J.; Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001, 25, 402–408. [Google Scholar] [CrossRef]
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Figure 1. Transgenic RNA interference (RNAi) approach downregulates Awd. (A) The wing disc, like the other imaginal discs, is a sac-like structure (x-z cross section along the vertical plane indicated by red dots) composed of a single layered epithelium whose morphology matures during larval development (x-y frontal section). The dashed lines mark the boundary between anterior (A, grey) and posterior (P, green) compartments as well as the dorso-ventral (DV) boundary. (B) Illustration of the UAS/Gal4 expression system applied to drive targeted coexpression of the UAS-awdi and UAS-GFP transgenes. (C,D) Confocal microscopy image of wing disc dissected from en>awdi larvae and immunostained for Awd (red in C,D). (E) qRT-PCR analysis of awd transcript level in cephalic complexes and wing discs dissected from larvae of the reported genotypes. Graphs represent mean ± SD; n = 3; ** = p < 0.01.
Figure 1. Transgenic RNA interference (RNAi) approach downregulates Awd. (A) The wing disc, like the other imaginal discs, is a sac-like structure (x-z cross section along the vertical plane indicated by red dots) composed of a single layered epithelium whose morphology matures during larval development (x-y frontal section). The dashed lines mark the boundary between anterior (A, grey) and posterior (P, green) compartments as well as the dorso-ventral (DV) boundary. (B) Illustration of the UAS/Gal4 expression system applied to drive targeted coexpression of the UAS-awdi and UAS-GFP transgenes. (C,D) Confocal microscopy image of wing disc dissected from en>awdi larvae and immunostained for Awd (red in C,D). (E) qRT-PCR analysis of awd transcript level in cephalic complexes and wing discs dissected from larvae of the reported genotypes. Graphs represent mean ± SD; n = 3; ** = p < 0.01.
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Figure 2. Posterior compartment reduction in en>awdi wing disc and adult wing. (A–D) Fluorescence microscopy image of wing discs dissected from en>+ (A,B) and en>awdi (C,D) larvae. Wing discs are labelled by DAPI (A,C) and GFP (B–D). Quantification of the average of the posterior area (GFP)/total area ratio in wing discs (E). Light micrographs of adult wings dissected from en>+ (F) and en>awdi (G) males. Quantification of the average of the posterior area (GFP)/total area ratio in adult wings (H). The red line marks the boundary between the anterior and posterior (P) compartments. The graphs represent the mean ± SD; **** = p < 0.0001.
Figure 2. Posterior compartment reduction in en>awdi wing disc and adult wing. (A–D) Fluorescence microscopy image of wing discs dissected from en>+ (A,B) and en>awdi (C,D) larvae. Wing discs are labelled by DAPI (A,C) and GFP (B–D). Quantification of the average of the posterior area (GFP)/total area ratio in wing discs (E). Light micrographs of adult wings dissected from en>+ (F) and en>awdi (G) males. Quantification of the average of the posterior area (GFP)/total area ratio in adult wings (H). The red line marks the boundary between the anterior and posterior (P) compartments. The graphs represent the mean ± SD; **** = p < 0.0001.
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Figure 3. Caspase 3 activation following awdi is unaffected by coexpression of p53DN. Fluorescence microscopy image of wing disc dissected from en>awdi (A–C) and en>p53DN, awdi (D–F) larvae. Wing discs are labelled by DAPI (A,D), anti-activated Casp3 (B,C,E,F) and GFP (C–F). White dots outline wing discs. Quantification of the CASP3-positive wing discs (G). The graphs represent the mean ± SD; n.s.: not statistically significant.
Figure 3. Caspase 3 activation following awdi is unaffected by coexpression of p53DN. Fluorescence microscopy image of wing disc dissected from en>awdi (A–C) and en>p53DN, awdi (D–F) larvae. Wing discs are labelled by DAPI (A,D), anti-activated Casp3 (B,C,E,F) and GFP (C–F). White dots outline wing discs. Quantification of the CASP3-positive wing discs (G). The graphs represent the mean ± SD; n.s.: not statistically significant.
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Figure 4. Analysis of Notch signaling in awd−/− clones and en>awdi wing discs. (A–C) Fluorescence microscopy image of wing disc dissected from larvae carrying awdJ2A4 MARCM clones (awd−/−) marked by GFP expression (C) and the GBE-lacZ transcriptional reporter for Notch activity. Wing discs are labelled by DAPI (A), anti-β-Gal (B,C) and GFP (C). (D–F) Fluorescence microscopy image of wing disc dissected from en>awdi larvae carrying the NRE-eGFP (E,F) transcriptional reporter for Notch activity. Wing discs are labelled by DAPI (D) and RFP marking the posterior domain (F). White dots outline wing discs, white arrowheads mark respectively the boundary of the MARCM clone (B,C) or the boundary of the posterior compartment (E,F).
Figure 4. Analysis of Notch signaling in awd−/− clones and en>awdi wing discs. (A–C) Fluorescence microscopy image of wing disc dissected from larvae carrying awdJ2A4 MARCM clones (awd−/−) marked by GFP expression (C) and the GBE-lacZ transcriptional reporter for Notch activity. Wing discs are labelled by DAPI (A), anti-β-Gal (B,C) and GFP (C). (D–F) Fluorescence microscopy image of wing disc dissected from en>awdi larvae carrying the NRE-eGFP (E,F) transcriptional reporter for Notch activity. Wing discs are labelled by DAPI (D) and RFP marking the posterior domain (F). White dots outline wing discs, white arrowheads mark respectively the boundary of the MARCM clone (B,C) or the boundary of the posterior compartment (E,F).
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Figure 5. awdi impairs Wg signaling. Confocal microscopy images of wing discs dissected from en>awdi larvae. Wing discs are labelled by anti-Awd (A,B), anti-Wg (A–E), anti-Sens (D,F). Yellow boxes in (A,D) outline the enlarged area showed in (B,C,E,F) respectively. White dots outline the boundary between anterior and posterior compartments (B,C,E,F). White arrowheads mark large aggregates of Wg protein, empty arrowheads mark Wg spots (B,C).
Figure 5. awdi impairs Wg signaling. Confocal microscopy images of wing discs dissected from en>awdi larvae. Wing discs are labelled by anti-Awd (A,B), anti-Wg (A–E), anti-Sens (D,F). Yellow boxes in (A,D) outline the enlarged area showed in (B,C,E,F) respectively. White dots outline the boundary between anterior and posterior compartments (B,C,E,F). White arrowheads mark large aggregates of Wg protein, empty arrowheads mark Wg spots (B,C).
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Figure 6. Impact of Awd levels on Notch and Wg signaling pathways. Schematic showing the impact of reduced (solid lines) and null (dashed line) Awd levels in cells at the DV boundary of wing disc. Grey lines show working hypothesis on the Awd involvement in recycling back the Evi chaperonin.
Figure 6. Impact of Awd levels on Notch and Wg signaling pathways. Schematic showing the impact of reduced (solid lines) and null (dashed line) Awd levels in cells at the DV boundary of wing disc. Grey lines show working hypothesis on the Awd involvement in recycling back the Evi chaperonin.
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Serafini, G.; Giordani, G.; Grillini, L.; Andrenacci, D.; Gargiulo, G.; Cavaliere, V. The Impact of Drosophila Awd/NME1/2 Levels on Notch and Wg Signaling Pathways. Int. J. Mol. Sci. 2020, 21, 7257. https://doi.org/10.3390/ijms21197257

AMA Style

Serafini G, Giordani G, Grillini L, Andrenacci D, Gargiulo G, Cavaliere V. The Impact of Drosophila Awd/NME1/2 Levels on Notch and Wg Signaling Pathways. International Journal of Molecular Sciences. 2020; 21(19):7257. https://doi.org/10.3390/ijms21197257

Chicago/Turabian Style

Serafini, Giulia, Giorgia Giordani, Luca Grillini, Davide Andrenacci, Giuseppe Gargiulo, and Valeria Cavaliere. 2020. "The Impact of Drosophila Awd/NME1/2 Levels on Notch and Wg Signaling Pathways" International Journal of Molecular Sciences 21, no. 19: 7257. https://doi.org/10.3390/ijms21197257

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