Custodiol-N Is Superior to Custodiol® Solution in Experimental Rat Uterus Preservation
Abstract
:1. Introduction
2. Results
2.1. Histology
2.2. Tissue MPO Expression
2.3. Tissue SOD Activity
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Uterus Procurement
4.3. Experimental Groups, Static Cold Storage (SCS), and Sampling
4.4. Histology
4.5. Immunohistochemical (IHC) Staining
4.6. Biochemistry
4.7. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
AUFI | absolute uterine factor infertility |
UTx | uterus transplantation |
H&E | hematoxylin and eosin |
IHC | immunohistochemistry |
IRI | ischemia/reperfusion injury |
MPO | myeloperoxidase |
ROS | reactive oxygen species |
SCS | static cold storage |
SOD | superoxide dismutase |
References
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Components (mmol/L) | Custodiol® | Custodiol-N Base Solution | Custodiol-N |
---|---|---|---|
Sodium | 16 | 16 | 16 |
Potassium | 10 | 10 | 10 |
Magnesium | 4 | 8 | 8 |
Calcium | 0.015 | 0.02 | 0.02 |
Chloride | 50 | 30 | 30 |
L-Histidine | 198 | 124 | 124 |
N-α-acetyl-L-Histidine | – | 57 | 57 |
Aspartate | 1 | 5 | 5 |
Tryptophan | 2 | 2 | 2 |
α-Ketoglutarate | 2 | 2 | 2 |
Arginine | – | 3 | 3 |
Alanine | – | 5 | 5 |
Sucrose | – | 33 | 33 |
Mannitol | 30 | – | – |
Glycine | – | 10 | 10 |
Deferoxamine | – | – | 0.025 |
LK-615 | – | – | 0.0075 |
Histological Findings | Score | |||
---|---|---|---|---|
0 | 1 | 2 | 3 | |
Edema | <5% | <5–15% | 15–30% | >30% |
Necrosis | Absent | <15% | 15–30% | >30% |
Smooth muscle contraction | Absent | Present | ||
Impaired basement membrane integrity | Absent | Present | ||
Endometrial cells loss | Absent | Present |
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Zitkute, V.; Kvietkauskas, M.; Maskoliunaite, V.; Leber, B.; Ramasauskaite, D.; Strupas, K.; Stiegler, P.; Schemmer, P. Custodiol-N Is Superior to Custodiol® Solution in Experimental Rat Uterus Preservation. Int. J. Mol. Sci. 2020, 21, 8015. https://doi.org/10.3390/ijms21218015
Zitkute V, Kvietkauskas M, Maskoliunaite V, Leber B, Ramasauskaite D, Strupas K, Stiegler P, Schemmer P. Custodiol-N Is Superior to Custodiol® Solution in Experimental Rat Uterus Preservation. International Journal of Molecular Sciences. 2020; 21(21):8015. https://doi.org/10.3390/ijms21218015
Chicago/Turabian StyleZitkute, Viktorija, Mindaugas Kvietkauskas, Vygante Maskoliunaite, Bettina Leber, Diana Ramasauskaite, Kestutis Strupas, Philipp Stiegler, and Peter Schemmer. 2020. "Custodiol-N Is Superior to Custodiol® Solution in Experimental Rat Uterus Preservation" International Journal of Molecular Sciences 21, no. 21: 8015. https://doi.org/10.3390/ijms21218015