Glycine Receptor Inhibition Differentially Affect Selected Neuronal Populations of the Developing Embryonic Cortex, as Evidenced by the Analysis of Spontaneous Calcium Oscillations
Abstract
:1. Introduction
2. Results
2.1. Spontaneous Calcium Activity in Developing Cortical Projection Neurons Is Differentially Affected along the Cortical Wall by the Inhibition of Glycinergic Neurotransmission
2.2. Migratory Interneurons Display Irregular Spontaneous Calcium Oscillations That Fluctuate in a Narrower Range upon Glycine Receptor Blockage
2.3. Power Spectral Analysis of Calcium Oscillations Reveals New Insights on the Effect of Neurotransmitter Receptor Inhibition in Interneurons
2.4. Gini Coefficient-Based Inequality Calculation of Power Spectrum Pinpoint Differences Accounting for the Effect of Interfering with Calcium Oscillations in Migrating Interneurons
2.5. Principal a Reveals Clustering by Cell Type and Treatment Effect on Cortical Neurons
3. Discussion
4. Material and Methods
4.1. Image Intensity Data Collection
4.2. Statistical Analysis
4.3. Sample Entropy and Hurst Exponent Calculation
4.4. Power spectral Analysis
4.5. Spectral Gini Coefficient Calculation
4.6. Principal Component Analysis (PCA)
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Ávila, D.; Aedo, E.; Sánchez-Hechavarria, M.; Ávila, C.; Ávila, A. Glycine Receptor Inhibition Differentially Affect Selected Neuronal Populations of the Developing Embryonic Cortex, as Evidenced by the Analysis of Spontaneous Calcium Oscillations. Int. J. Mol. Sci. 2020, 21, 8013. https://doi.org/10.3390/ijms21218013
Ávila D, Aedo E, Sánchez-Hechavarria M, Ávila C, Ávila A. Glycine Receptor Inhibition Differentially Affect Selected Neuronal Populations of the Developing Embryonic Cortex, as Evidenced by the Analysis of Spontaneous Calcium Oscillations. International Journal of Molecular Sciences. 2020; 21(21):8013. https://doi.org/10.3390/ijms21218013
Chicago/Turabian StyleÁvila, Denisse, Eduardo Aedo, Miguel Sánchez-Hechavarria, Claudio Ávila, and Ariel Ávila. 2020. "Glycine Receptor Inhibition Differentially Affect Selected Neuronal Populations of the Developing Embryonic Cortex, as Evidenced by the Analysis of Spontaneous Calcium Oscillations" International Journal of Molecular Sciences 21, no. 21: 8013. https://doi.org/10.3390/ijms21218013