Bacteria Residing at Root Canals Can Induce Cell Proliferation and Alter the Mechanical Properties of Gingival and Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. Stimulation of Gingival and Cancer Cells with Heat-Inactivated Bacteria
2.2. Stimulation of Cells with Biofilm-Collected Supernatants
2.3. Proliferation Capability of Gingival and Cancer Cells Stimulated with Bacterial Biofilms Formed in the Presence of Tooth Tissue
2.4. Exposure of Gingival and Cancer Cells to Heat-Activated Bacteria and Biofilm-Collected Supernatants Increase the Number of Ki-67-Positive Cells
2.5. Morphological and Nanomechanical Features of Treated Gingival and Lung Cancer Cells
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Preparation of Bacterial Strains
4.3. Cell Culture
4.4. Evaluation of Proliferation Capability of Treated Cells
4.5. Stimulation of Cells with Heat-Inactivated Bacteria
4.6. Stimulation of Cells with Biofilm-Derived Solutions
4.7. Formation of Biofilms in Both Aerobic and Anaerobic Conditions
4.8. Stimulation of Cells with Biofilm-Derived Supernatants Formed in the Presence of Bone Tissue
4.9. Measurement of Ki-67 Expression
4.10. AFM Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
A. odontolyticus ATCC 17929 | Actinomyces odontolyticus bacteria strain no. 17929 |
E. faecalis ATCC 29211 | Enterococcus faecalis bacteria strain no. 29211 |
P. acnes ATCC 11827 | Propionibacterium acnes bacteria strain no. 11827 |
A549 | human lung adenocarcinoma; cell line isolated from a pulmonary adenocarcinoma |
DMEM | Dulbecco’s modified Eagle’s medium |
HGF | primary gingival fibroblast cell line derived from adult gingival tissue |
MCF-7 | human breast adenocarcinoma |
PBS | phosphate-buffered saline |
SKOV-3 | human ovarian adenocarcinoma |
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Suprewicz, Ł.; Tokajuk, G.; Cieśluk, M.; Deptuła, P.; Sierpińska, T.; Wolak, P.; Wollny, T.; Tokajuk, J.; Głuszek, S.; Piktel, E.; et al. Bacteria Residing at Root Canals Can Induce Cell Proliferation and Alter the Mechanical Properties of Gingival and Cancer Cells. Int. J. Mol. Sci. 2020, 21, 7914. https://doi.org/10.3390/ijms21217914
Suprewicz Ł, Tokajuk G, Cieśluk M, Deptuła P, Sierpińska T, Wolak P, Wollny T, Tokajuk J, Głuszek S, Piktel E, et al. Bacteria Residing at Root Canals Can Induce Cell Proliferation and Alter the Mechanical Properties of Gingival and Cancer Cells. International Journal of Molecular Sciences. 2020; 21(21):7914. https://doi.org/10.3390/ijms21217914
Chicago/Turabian StyleSuprewicz, Łukasz, Grażyna Tokajuk, Mateusz Cieśluk, Piotr Deptuła, Teresa Sierpińska, Przemysław Wolak, Tomasz Wollny, Joanna Tokajuk, Stanisław Głuszek, Ewelina Piktel, and et al. 2020. "Bacteria Residing at Root Canals Can Induce Cell Proliferation and Alter the Mechanical Properties of Gingival and Cancer Cells" International Journal of Molecular Sciences 21, no. 21: 7914. https://doi.org/10.3390/ijms21217914