High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai
Abstract
:1. Introduction
2. Results and Discussions
3. Materials and Methods
3.1. Synthesis of seMpai
3.2. Cell Culture
3.3. Animal Studies
3.4. pH Measurement of Luciferin Analogues
3.5. Bioluminescence Measurement of seMpai-AkaLuc Reaction
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
BLI | Bioluminescence imaging |
NIR | Near-infrared |
Aka-BLI | AkaLumine-AkaLuc bioluminescence imaging |
HCl | Hydrochloric acid |
ERBB2 | Erb-b2 receptor tyrosine kinase 2 |
PBS | Phosphate Buffered Saline |
KPB | Potassium phosphate buffer |
ATP | Adenosine triphosphate |
ROI | Region of interests |
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Nakayama, J.; Saito, R.; Hayashi, Y.; Kitada, N.; Tamaki, S.; Han, Y.; Semba, K.; Maki, S.A. High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai. Int. J. Mol. Sci. 2020, 21, 7896. https://doi.org/10.3390/ijms21217896
Nakayama J, Saito R, Hayashi Y, Kitada N, Tamaki S, Han Y, Semba K, Maki SA. High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai. International Journal of Molecular Sciences. 2020; 21(21):7896. https://doi.org/10.3390/ijms21217896
Chicago/Turabian StyleNakayama, Jun, Ryohei Saito, Yusuke Hayashi, Nobuo Kitada, Shota Tamaki, Yuxuan Han, Kentaro Semba, and Shojiro A. Maki. 2020. "High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai" International Journal of Molecular Sciences 21, no. 21: 7896. https://doi.org/10.3390/ijms21217896
APA StyleNakayama, J., Saito, R., Hayashi, Y., Kitada, N., Tamaki, S., Han, Y., Semba, K., & Maki, S. A. (2020). High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai. International Journal of Molecular Sciences, 21(21), 7896. https://doi.org/10.3390/ijms21217896