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High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

1
Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Tokyo 162-8480, Japan
2
Division of Cellular Signaling, National Cancer Center Research Institute, Tokyo 104-0045, Japan
3
School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo 192-0392, Japan
4
Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro-Communications, Tokyo 182-8585, Japan
5
Center for Neuroscience and Biomedical Engineering, The University of Electro-Communications, Tokyo 182-8585, Japan
6
Department of Cell Factory, Translational Research Center, Fukushima Medical University, Fukushima 960-1295, Japan
*
Author to whom correspondence should be addressed.
J.N. and R.S. contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(21), 7896; https://doi.org/10.3390/ijms21217896
Received: 16 October 2020 / Revised: 22 October 2020 / Accepted: 23 October 2020 / Published: 24 October 2020
Bioluminescence imaging (BLI) is useful to monitor cell movement and gene expression in live animals. However, D-luciferin has a short wavelength (560 nm) which is absorbed by tissues and the use of near-infrared (NIR) luciferin analogues enable high sensitivity in vivo BLI. The AkaLumine-AkaLuc BLI system (Aka-BLI) can detect resolution at the single-cell level; however, it has a clear hepatic background signal. Here, to enable the highly sensitive detection of bioluminescence from the surrounding liver tissues, we focused on seMpai (C15H16N3O2S) which has been synthesized as a luciferin analogue and has high luminescent abilities as same as AkaLumine. We demonstrated that seMpai BLI could detect micro-signals near the liver without any background signal. The solution of seMpai was neutral; therefore, seMpai imaging did not cause any adverse effect in mice. seMpai enabled a highly sensitive in vivo BLI as compared to previous techniques. Our findings suggest that the development of a novel mutated luciferase against seMpai may enable a highly sensitive BLI at the single-cell level without any background signal. Novel seMpai BLI system can be used for in vivo imaging in the fields of life sciences and medicine. View Full-Text
Keywords: in vivo imaging; near-infrared bioluminescence; luciferin analogue; metastasis in vivo imaging; near-infrared bioluminescence; luciferin analogue; metastasis
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MDPI and ACS Style

Nakayama, J.; Saito, R.; Hayashi, Y.; Kitada, N.; Tamaki, S.; Han, Y.; Semba, K.; Maki, S.A. High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai. Int. J. Mol. Sci. 2020, 21, 7896. https://doi.org/10.3390/ijms21217896

AMA Style

Nakayama J, Saito R, Hayashi Y, Kitada N, Tamaki S, Han Y, Semba K, Maki SA. High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai. International Journal of Molecular Sciences. 2020; 21(21):7896. https://doi.org/10.3390/ijms21217896

Chicago/Turabian Style

Nakayama, Jun; Saito, Ryohei; Hayashi, Yusuke; Kitada, Nobuo; Tamaki, Shota; Han, Yuxuan; Semba, Kentaro; Maki, Shojiro A. 2020. "High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai" Int. J. Mol. Sci. 21, no. 21: 7896. https://doi.org/10.3390/ijms21217896

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