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Epigenetic Mechanisms of Plant Adaptation to Biotic and Abiotic Stresses
Review

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

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Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Av. Puerta de Hierro, nº 2–4, 28040 Madrid, Spain
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Departamento de Economía Agraria, Estadística y Gestión de Empresas, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Av. Puerta de Hierro, nº 2–4, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(20), 7459; https://doi.org/10.3390/ijms21207459
Received: 22 August 2020 / Revised: 29 September 2020 / Accepted: 8 October 2020 / Published: 10 October 2020
Epigenetic variation, and particularly DNA methylation, is involved in plasticity and responses to changes in the environment. Conservation biology studies have focused on the measurement of this variation to establish demographic parameters, diversity levels and population structure to design the appropriate conservation strategies. However, in ex situ conservation approaches, the main objective is to guarantee the characteristics of the conserved material (phenotype and epi-genetic). We review the use of the Methylation Sensitive Amplified Polymorphism (MSAP) technique to detect changes in the DNA methylation patterns of plant material conserved by the main ex situ plant conservation methods: seed banks, in vitro slow growth and cryopreservation. Comparison of DNA methylation patterns before and after conservation is a useful tool to check the fidelity of the regenerated plants, and, at the same time, may be related with other genetic variations that might appear during the conservation process (i.e., somaclonal variation). Analyses of MSAP profiles can be useful in the management of ex situ plant conservation but differs in the approach used in the in situ conservation. Likewise, an easy-to-use methodology is necessary for a rapid interpretation of data, in order to be readily implemented by conservation managers. View Full-Text
Keywords: cryopreservation; DNA methylation; multinomial model; plant tissue culture; seed storage cryopreservation; DNA methylation; multinomial model; plant tissue culture; seed storage
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MDPI and ACS Style

González-Benito, M.E.; Ibáñez, M.Á.; Pirredda, M.; Mira, S.; Martín, C. Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation. Int. J. Mol. Sci. 2020, 21, 7459. https://doi.org/10.3390/ijms21207459

AMA Style

González-Benito ME, Ibáñez MÁ, Pirredda M, Mira S, Martín C. Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation. International Journal of Molecular Sciences. 2020; 21(20):7459. https://doi.org/10.3390/ijms21207459

Chicago/Turabian Style

González-Benito, María E., Miguel Á. Ibáñez, Michela Pirredda, Sara Mira, and Carmen Martín. 2020. "Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation" International Journal of Molecular Sciences 21, no. 20: 7459. https://doi.org/10.3390/ijms21207459

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