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Cadmium-Related Effects on Cellular Immunity Comprises Altered Metabolism in Earthworm Coelomocytes

Institute of Zoology, Center for Molecular Biosciences Innsbruck, University of Innsbruck, 25 6020 Technikerstraße, Austria
Department of Cell Biology, Adaptive Biology Laboratory, Universidade Federal do Paraná, Curitiba, Paraná 82590-300, Brazil
Institute for Biomedical Aging Research, University of Innsbruck, 6020 Innsbruck, Austria
Environmental Proteomics Laboratory, Department of Biological Sciences, Center for Coastal Marine Studies, California Polytechnic State University, 1 Grand Ave., San Luis Obispo, CA 93407-0401, USA
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(2), 599;
Received: 23 December 2019 / Revised: 9 January 2020 / Accepted: 13 January 2020 / Published: 17 January 2020
(This article belongs to the Special Issue Advances in Metal Metabolism Research)
The heavy metal cadmium (Cd) is known to modulate the immune system, challenging soil-dwelling organisms where environmental Cd pollution is high. Since earthworms lack adaptive immunity, we determined Cd-related effects on coelomocytes, the cellular part of innate immunity, which is also the site of detoxification processes. A proteomics approach revealed a set of immunity-related proteins as well as gene products involved in energy metabolism changing in earthworms in response to Cd exposure. Based on these results, we conducted extracellular flux measurements of oxygen and acidification to reveal the effect of Cd on coelomocyte metabolism. We observed a significantly changing oxygen consumption rate, extracellular acidification, as well as metabolic potential, which can be defined as the response to an induced energy demand. Acute changes in intracellular calcium levels were also observed, indicating impaired coelomocyte activation. Lysosomes, the cell protein recycling center, and mitochondrial parameters did not change. Taken together, we were able to characterize coelomocyte metabolism to reveal a potential link to an impaired immune system upon Cd exposure. View Full-Text
Keywords: coelomocytes; earthworm; metabolism coelomocytes; earthworm; metabolism
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MDPI and ACS Style

Höckner, M.; Piechnik, C.A.; Fiechtner, B.; Weinberger, B.; Tomanek, L. Cadmium-Related Effects on Cellular Immunity Comprises Altered Metabolism in Earthworm Coelomocytes. Int. J. Mol. Sci. 2020, 21, 599.

AMA Style

Höckner M, Piechnik CA, Fiechtner B, Weinberger B, Tomanek L. Cadmium-Related Effects on Cellular Immunity Comprises Altered Metabolism in Earthworm Coelomocytes. International Journal of Molecular Sciences. 2020; 21(2):599.

Chicago/Turabian Style

Höckner, Martina, Claudio A. Piechnik, Birgit Fiechtner, Birgit Weinberger, and Lars Tomanek. 2020. "Cadmium-Related Effects on Cellular Immunity Comprises Altered Metabolism in Earthworm Coelomocytes" International Journal of Molecular Sciences 21, no. 2: 599.

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