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Article

Effect of Oral Losartan on Orthobiologics: Implications for Platelet-Rich Plasma and Bone Marrow Concentrate—A Rabbit Study

1
Center for Regenerative Sports Medicine, Steadman Philippon Research Institute, Vail, CO 81657, USA
2
Department of Orthopaedic Surgery, University of Texas Health Science Center at Houston, Houston, TX 77054, USA
3
The Steadman Clinic, Vail, CO 81657, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(19), 7374; https://doi.org/10.3390/ijms21197374
Received: 10 September 2020 / Revised: 29 September 2020 / Accepted: 1 October 2020 / Published: 6 October 2020
(This article belongs to the Special Issue Regenerative Therapy Using Blood-Derived Biomaterials)
Recent efforts have focused on customizing orthobiologics, such as platelet-rich plasma (PRP) and bone marrow concentrate (BMC), to improve tissue repair. We hypothesized that oral losartan (a TGF-β1 blocker with anti-fibrotic properties) could decrease TGF-β1 levels in leukocyte-poor PRP (LP-PRP) and fibrocytes in BMC. Ten rabbits were randomized into two groups (N = 5/group): osteochondral defect + microfracture (control, group 1) and osteochondral defect + microfracture + losartan (losartan, group 2). For group 2, a dose of 10mg/kg/day of losartan was administrated orally for 12 weeks post-operatively. After 12 weeks, whole blood (WB) and bone marrow aspirate (BMA) samples were collected to process LP-PRP and BMC. TGF-β1 concentrations were measured in WB and LP-PRP with multiplex immunoassay. BMC cell populations were analyzed by flow cytometry with CD31, CD44, CD45, CD34, CD146 and CD90 antibodies. There was no significant difference in TGF-β1 levels between the losartan and control group in WB or LP-PRP. In BMC, the percentage of CD31+ cells (endothelial cells) in the losartan group was significantly higher than the control group (p = 0.008), while the percentage of CD45+ cells (hematopoietic cells-fibrocytes) in the losartan group was significantly lower than the control group (p = 0.03). View Full-Text
Keywords: fibrosis; losartan; transforming growth factor-1 beta (TGF-β1); bone marrow concentrate (BMC); leukocyte-poor platelet-rich plasma (LP-PRP) fibrosis; losartan; transforming growth factor-1 beta (TGF-β1); bone marrow concentrate (BMC); leukocyte-poor platelet-rich plasma (LP-PRP)
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MDPI and ACS Style

Nakama, G.Y.; Gonzalez, S.; Matre, P.; Mu, X.; Whitney, K.E.; Utsunomiya, H.; Arner, J.W.; Philippon, M.J.; Ravuri, S.; Huard, J. Effect of Oral Losartan on Orthobiologics: Implications for Platelet-Rich Plasma and Bone Marrow Concentrate—A Rabbit Study. Int. J. Mol. Sci. 2020, 21, 7374. https://doi.org/10.3390/ijms21197374

AMA Style

Nakama GY, Gonzalez S, Matre P, Mu X, Whitney KE, Utsunomiya H, Arner JW, Philippon MJ, Ravuri S, Huard J. Effect of Oral Losartan on Orthobiologics: Implications for Platelet-Rich Plasma and Bone Marrow Concentrate—A Rabbit Study. International Journal of Molecular Sciences. 2020; 21(19):7374. https://doi.org/10.3390/ijms21197374

Chicago/Turabian Style

Nakama, Gilberto Y., Sabrina Gonzalez, Polina Matre, Xiaodong Mu, Kaitlyn E. Whitney, Hajime Utsunomiya, Justin W. Arner, Marc J. Philippon, Sudheer Ravuri, and Johnny Huard. 2020. "Effect of Oral Losartan on Orthobiologics: Implications for Platelet-Rich Plasma and Bone Marrow Concentrate—A Rabbit Study" International Journal of Molecular Sciences 21, no. 19: 7374. https://doi.org/10.3390/ijms21197374

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