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Review

Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators

1
Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
2
Department of Chemistry, The University of Tokyo, Tokyo 113-0033, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(18), 6522; https://doi.org/10.3390/ijms21186522
Received: 25 August 2020 / Revised: 3 September 2020 / Accepted: 4 September 2020 / Published: 7 September 2020
Optogenetic (photo-responsive) actuators engineered from photoreceptors are widely used in various applications to study cell biology and tissue physiology. In the toolkit of optogenetic actuators, the key building blocks are genetically encodable light-sensitive proteins. Currently, most optogenetic photosensory modules are engineered from naturally-occurring photoreceptor proteins from bacteria, fungi, and plants. There is a growing demand for novel photosensory domains with improved optical properties and light-induced responses to satisfy the needs of a wider variety of studies in biological sciences. In this review, we focus on progress towards engineering of non-opsin-based photosensory domains, and their representative applications in cell biology and physiology. We summarize current knowledge of engineering of light-sensitive proteins including light-oxygen-voltage-sensing domain (LOV), cryptochrome (CRY2), phytochrome (PhyB and BphP), and fluorescent protein (FP)-based photosensitive domains (Dronpa and PhoCl). View Full-Text
Keywords: optogenetic actuator; protein engineering; photosensory domains optogenetic actuator; protein engineering; photosensory domains
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MDPI and ACS Style

Lu, X.; Shen, Y.; Campbell, R.E. Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators. Int. J. Mol. Sci. 2020, 21, 6522. https://doi.org/10.3390/ijms21186522

AMA Style

Lu X, Shen Y, Campbell RE. Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators. International Journal of Molecular Sciences. 2020; 21(18):6522. https://doi.org/10.3390/ijms21186522

Chicago/Turabian Style

Lu, Xiaocen, Yi Shen, and Robert E. Campbell 2020. "Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators" International Journal of Molecular Sciences 21, no. 18: 6522. https://doi.org/10.3390/ijms21186522

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