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Open AccessArticle

The Regulatory Network of CMPG1-V in Wheat–Blumeria graminis f. sp. tritici Interaction Revealed by Temporal Profiling Using RNA-Seq

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China
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Author to whom correspondence should be addressed.
These authors contribute equally to this work.
Int. J. Mol. Sci. 2020, 21(17), 5967; https://doi.org/10.3390/ijms21175967
Received: 16 July 2020 / Revised: 9 August 2020 / Accepted: 17 August 2020 / Published: 19 August 2020
(This article belongs to the Special Issue Wheat and Barley: Acclimatization to Abiotic and Biotic Stress)
Wheat powdery mildew (Pm), caused by Blumeria graminis f. sp. tritici (Bgt), is a prevalent fungal disease. The diploid wheat relative Haynaldia villosa (H. villosa) showed broad-spectrum resistance (BSR) to Pm. A previous study reported an E3 ligase gene, CMPG1-V from H. villosa, showing BSR to Pm. To elucidate the regulatory network mediated by CMPG1-V, in this study, gene expression profiling of CMPG1-V transgenic plant (CMPG1-VOE) and its receptor Yangmai 158 was analyzed and compared after Bgt inoculation at four infection stages. GO and KEGG analysis revealed obvious reprogramming of SA and ABA signaling, starch/sucrose metabolism, and photosynthesis in CMPG1-VOE, compared with those in Yangmai 158. Transcripts of SA synthesis genes SARD1 and UGT, signaling factors TGA and PRs, and SnRKs in ABA signaling were specifically upregulated in CMPG1-VOE rather than Yangmai 158. Transcripts of LHCII in photosynthesis, GLUC and TPP in starch/sucrose metabolism were also induced distinctly in CMPG1-VOE. WGCNA analysis showed crucial regulatory candidates of CMPG1-V, involving serine/threonine-protein kinase in phosphorylation, glucosyltransferase in flavonoid biosynthesis, defense factor WRKYs, and peroxidase in oxidative stress. Our results facilitate the deciphering of the resistant regulatory network of CMPG1-V and the identification of key candidates which might be employed in breeding programs. View Full-Text
Keywords: wheat powdery mildew; Haynaldia villosa; CMPG1-V; RNA-seq; hormone signaling; metabolism process wheat powdery mildew; Haynaldia villosa; CMPG1-V; RNA-seq; hormone signaling; metabolism process
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MDPI and ACS Style

Liu, J.; Sun, L.; Chen, Y.; Wei, L.; Hao, Y.; Yu, Z.; Wang, Z.; Zhang, H.; Zhang, X.; Li, M.; Wang, H.; Xiao, J.; Wang, X. The Regulatory Network of CMPG1-V in Wheat–Blumeria graminis f. sp. tritici Interaction Revealed by Temporal Profiling Using RNA-Seq. Int. J. Mol. Sci. 2020, 21, 5967. https://doi.org/10.3390/ijms21175967

AMA Style

Liu J, Sun L, Chen Y, Wei L, Hao Y, Yu Z, Wang Z, Zhang H, Zhang X, Li M, Wang H, Xiao J, Wang X. The Regulatory Network of CMPG1-V in Wheat–Blumeria graminis f. sp. tritici Interaction Revealed by Temporal Profiling Using RNA-Seq. International Journal of Molecular Sciences. 2020; 21(17):5967. https://doi.org/10.3390/ijms21175967

Chicago/Turabian Style

Liu, Jia; Sun, Li; Chen, Yiming; Wei, Luyang; Hao, Yongli; Yu, Zhongyu; Wang, Zongkuan; Zhang, Heng; Zhang, Xu; Li, Mengli; Wang, Haiyan; Xiao, Jin; Wang, Xiue. 2020. "The Regulatory Network of CMPG1-V in Wheat–Blumeria graminis f. sp. tritici Interaction Revealed by Temporal Profiling Using RNA-Seq" Int. J. Mol. Sci. 21, no. 17: 5967. https://doi.org/10.3390/ijms21175967

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