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Article

Carbon Fibers as a New Type of Scaffold for Midbrain Organoid Development

1
Department of Transplantation, Faculty of Medicine, Institute of Pediatrics, Jagiellonian University Medical College, Wielicka 265, 30-663 Kraków, Poland
2
Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 5959; https://doi.org/10.3390/ijms21175959
Received: 30 June 2020 / Revised: 14 August 2020 / Accepted: 17 August 2020 / Published: 19 August 2020
(This article belongs to the Special Issue Cell-Biomaterial Interaction 2020)
The combination of induced pluripotent stem cell (iPSC) technology and 3D cell culture creates a unique possibility for the generation of organoids that mimic human organs in in vitro cultures. The use of iPS cells in organoid cultures enables the differentiation of cells into dopaminergic neurons, also found in the human midbrain. However, long-lasting organoid cultures often cause necrosis within organoids. In this work, we present carbon fibers (CFs) for medical use as a new type of scaffold for organoid culture, comparing them to a previously tested copolymer poly-(lactic-co-glycolic acid) (PLGA) scaffold. We verified the physicochemical properties of CF scaffolds compared to PLGA in improving the efficiency of iPSC differentiation within organoids. The physicochemical properties of carbon scaffolds such as porosity, microstructure, or stability in the cellular environment make them a convenient material for creating in vitro organoid models. Through screening several genes expressed during the differentiation of organoids at crucial brain stages of development, we found that there is a correlation between PITX3, one of the key regulators of terminal differentiation, and the survival of midbrain dopaminergic (mDA) neurons and tyrosine hydroxylase (TH) gene expression. This makes organoids formed on carbon scaffolds an improved model containing mDA neurons convenient for studying midbrain-associated neurodegenerative diseases such as Parkinson’s disease. View Full-Text
Keywords: organoid; Parkinson’s disease; carbon fibers; scaffold; 3D model; NURR1; LMX1A; TH; PITX3 organoid; Parkinson’s disease; carbon fibers; scaffold; 3D model; NURR1; LMX1A; TH; PITX3
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MDPI and ACS Style

Tejchman, A.; Znój, A.; Chlebanowska, P.; Frączek-Szczypta, A.; Majka, M. Carbon Fibers as a New Type of Scaffold for Midbrain Organoid Development. Int. J. Mol. Sci. 2020, 21, 5959. https://doi.org/10.3390/ijms21175959

AMA Style

Tejchman A, Znój A, Chlebanowska P, Frączek-Szczypta A, Majka M. Carbon Fibers as a New Type of Scaffold for Midbrain Organoid Development. International Journal of Molecular Sciences. 2020; 21(17):5959. https://doi.org/10.3390/ijms21175959

Chicago/Turabian Style

Tejchman, Anna, Agnieszka Znój, Paula Chlebanowska, Aneta Frączek-Szczypta, and Marcin Majka. 2020. "Carbon Fibers as a New Type of Scaffold for Midbrain Organoid Development" International Journal of Molecular Sciences 21, no. 17: 5959. https://doi.org/10.3390/ijms21175959

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