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Article

Upregulation of the TRPA1 Ion Channel in the Gastric Mucosa after Iodoacetamide-Induced Gastritis in Rats: A Potential New Therapeutic Target

1
Department of Pharmacology and Pharmacotherapy, Medical School, University of Pécs, H-7624 Pécs, Hungary
2
Szentagothai Research Centre, University of Pécs, H-7624 Pécs, Hungary
3
Institute for Translational Medicine, Medical School, University of Pécs, H-7624 Pécs, Hungary
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1st Department of Medicine, Medical School, University of Pécs, H-7624 Pécs, Hungary
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Department of Pathology, Medical School, University of Pécs, H-7624 Pécs, Hungary
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Hannover Medical School, Institute of Toxicology, Core Unit Proteomics, 30625 Hannover, Germany
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School of Medicine, University of California, Irvine, CA 92697, USA
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Department of Pharmaceutical Science, American University of Health Sciences, Signal Hill, CA 90755, USA
9
PharmInVivo Ltd., H-7629 Pécs, Hungary
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(16), 5591; https://doi.org/10.3390/ijms21165591
Received: 7 June 2020 / Revised: 20 July 2020 / Accepted: 28 July 2020 / Published: 5 August 2020
(This article belongs to the Special Issue TRP Channels)
Acute gastritis is often untreatable by acid secretion-inhibiting drugs. Understanding the protective mechanisms including the role of Transient Receptor Potential Ankyrin1 (TRPA1) and Vanilloid1 (TRPV1) channels localized on capsaicin-sensitive afferents and non-neuronal structures might identify novel therapeutic approaches. Therefore, we characterized a translational gastritis model using iodoacetamide (IAA) and investigated TRPA1/V1 expressions. Wistar rats and CD1, C57Bl/6J mice were exposed to IAA-containing (0.05, 0.1, 0.2, 0.3, 0.5%) drinking water for 7 or 14 days. Body weight and water consumption were recorded daily. Macroscopic lesions were scored, qualitative histopathologic investigation was performed, TRPA1/V1 immunopositivity and mRNA expressions were measured. IAA induced a concentration-dependent weight loss and reduced water intake in both species. Hyperemia, submucosal edema, inflammatory infiltration and hemorrhagic erosions developed after 7 days, while ulcers after 14 days in rats. Trpa1 mRNA/protein expressions were upregulated at both timepoints. Meanwhile, TRPV1 immunopositivity was upregulated in the gastric corpus after 0.05% IAA ingestion, but downregulated after 0.2%, whereas Trpv1 mRNA did not change. Interestingly, no macroscopic/microscopic changes were observed in mice. These are the first data for the concentration- and duration-dependent changes in the IAA-induced gastritis in rats accompanied by TRPA1 upregulation, therefore, its therapeutic potential in gastritis should further be investigated. View Full-Text
Keywords: TRPA1; TRV1; gastritis; inflammation; erosion; rodent TRPA1; TRV1; gastritis; inflammation; erosion; rodent
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MDPI and ACS Style

Csekő, K.; Pécsi, D.; Kajtár, B.; Hegedűs, I.; Bollenbach, A.; Tsikas, D.; Szabó, I.L.; Szabó, S.; Helyes, Z. Upregulation of the TRPA1 Ion Channel in the Gastric Mucosa after Iodoacetamide-Induced Gastritis in Rats: A Potential New Therapeutic Target. Int. J. Mol. Sci. 2020, 21, 5591. https://doi.org/10.3390/ijms21165591

AMA Style

Csekő K, Pécsi D, Kajtár B, Hegedűs I, Bollenbach A, Tsikas D, Szabó IL, Szabó S, Helyes Z. Upregulation of the TRPA1 Ion Channel in the Gastric Mucosa after Iodoacetamide-Induced Gastritis in Rats: A Potential New Therapeutic Target. International Journal of Molecular Sciences. 2020; 21(16):5591. https://doi.org/10.3390/ijms21165591

Chicago/Turabian Style

Csekő, Kata, Dániel Pécsi, Béla Kajtár, Ivett Hegedűs, Alexander Bollenbach, Dimitrios Tsikas, Imre L. Szabó, Sándor Szabó, and Zsuzsanna Helyes. 2020. "Upregulation of the TRPA1 Ion Channel in the Gastric Mucosa after Iodoacetamide-Induced Gastritis in Rats: A Potential New Therapeutic Target" International Journal of Molecular Sciences 21, no. 16: 5591. https://doi.org/10.3390/ijms21165591

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