Genome-Wide Analysis Reveals Changes in Long Noncoding RNAs in the Differentiation of Canine BMSCs into Insulin-Producing Cells
Abstract
:1. Introduction
2. Results
2.1. Overview of lncRNA Sequencing
2.2. Identification of lncRNAs
2.3. Differentially Expressed lncRNAs
2.4. Enrichment Analysis of Colocalized Genes
2.5. Enrichment Analysis of Coexpressed Genes
2.6. Verification of Target Genes and lncRNAs
3. Discussion
4. Materials and Methods
4.1. Separation, Cultivation, and Induction of cBMSCs
4.2. RNA Isolation and Qualification
4.3. Library Preparation
5. Sequencing
5.1. Transcriptome Assembly
5.2. Identification of lncRNAs
5.3. Differential Expression Analysis
5.4. GO and KEGG Enrichment Analyses
5.5. Target Gene Prediction
5.6. Gene Expression Validation by qRT-PCR
6. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wang, J.; Dai, P.; Gao, D.; Zhang, X.; Ruan, C.; Li, J.; Chen, Y.; Zhang, L.; Zhang, Y. Genome-Wide Analysis Reveals Changes in Long Noncoding RNAs in the Differentiation of Canine BMSCs into Insulin-Producing Cells. Int. J. Mol. Sci. 2020, 21, 5549. https://doi.org/10.3390/ijms21155549
Wang J, Dai P, Gao D, Zhang X, Ruan C, Li J, Chen Y, Zhang L, Zhang Y. Genome-Wide Analysis Reveals Changes in Long Noncoding RNAs in the Differentiation of Canine BMSCs into Insulin-Producing Cells. International Journal of Molecular Sciences. 2020; 21(15):5549. https://doi.org/10.3390/ijms21155549
Chicago/Turabian StyleWang, Jinglu, Pengxiu Dai, Dengke Gao, Xia Zhang, Chenmei Ruan, Jiakai Li, Yijing Chen, Luwen Zhang, and Yihua Zhang. 2020. "Genome-Wide Analysis Reveals Changes in Long Noncoding RNAs in the Differentiation of Canine BMSCs into Insulin-Producing Cells" International Journal of Molecular Sciences 21, no. 15: 5549. https://doi.org/10.3390/ijms21155549