Inhaled Argon Impedes Hepatic Regeneration after Ischemia/Reperfusion Injury in Rats
Abstract
:1. Introduction
2. Results
2.1. Effect of Argon on Hepatocytes Proliferation and Apoptosis after I/R
2.2. Argon Modulates the Expression of Cytokines
2.3. Plasma Biochemical Data and Parameters of Liver Function (Liver Specific Values)
2.4. Liver Injury after IRI Measured by Suzuki’s Score
3. Methods
3.1. Animals and Study Design
3.2. Anesthesia
3.3. Surgical Procedure and Postoperative Care
3.4. Histology
3.5. Immunohistochemistry
3.5.1. Bromodeoxyuridine
3.5.2. KI-67
3.5.3. TUNEL
3.6. RNA Isolation and Quantitative RT-PCR
3.7. Blood Samples
3.8. Quantification and Statistical Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Schmitz, S.M.; Dohmeier, H.; Stoppe, C.; Alizai, P.H.; Schipper, S.; Neumann, U.P.; Coburn, M.; Ulmer, T.F. Inhaled Argon Impedes Hepatic Regeneration after Ischemia/Reperfusion Injury in Rats. Int. J. Mol. Sci. 2020, 21, 5457. https://doi.org/10.3390/ijms21155457
Schmitz SM, Dohmeier H, Stoppe C, Alizai PH, Schipper S, Neumann UP, Coburn M, Ulmer TF. Inhaled Argon Impedes Hepatic Regeneration after Ischemia/Reperfusion Injury in Rats. International Journal of Molecular Sciences. 2020; 21(15):5457. https://doi.org/10.3390/ijms21155457
Chicago/Turabian StyleSchmitz, Sophia M., Henriette Dohmeier, Christian Stoppe, Patrick H. Alizai, Sandra Schipper, Ulf P. Neumann, Mark Coburn, and Tom F. Ulmer. 2020. "Inhaled Argon Impedes Hepatic Regeneration after Ischemia/Reperfusion Injury in Rats" International Journal of Molecular Sciences 21, no. 15: 5457. https://doi.org/10.3390/ijms21155457