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Open AccessArticle

Unusual Occurrence of Two Bona-Fide CCA-Adding Enzymes in Dictyostelium discoideum

1
Institute for Biochemistry, University of Leipzig, Brüderstraße 34, 04103 Leipzig, Germany
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Interdisciplinary Center for Bioinformatics, Leipzig University, Härtelstraße 16-18, 04107 Leipzig, Germany
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Ribogenetics Biochemistry Lab, Department of Life Sciences and Chemistry, Jacobs University Bremen gGmbH, Campus Ring 1, 28759 Bremen, Germany
4
German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Competence Center for Scalable Data Services and Solutions, and Leipzig Research Center for Civilization Diseases, Leipzig University, 04103 Leipzig, Germany
5
Max Planck Institute for Mathematics in the Sciences, 04103 Leipzig, Germany
6
Facultad de Ciencias, Universidad National de Colombia, Sede Bogotá, Carrera 45 No. 26-85, Colombia
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Santa Fe Institute for Complex Systems, 1399 Hyde Park Road, Santa Fe, NM 87501, USA
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Department of Theoretical Chemistry of the University of Vienna, A-1090 Vienna, Austria
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Computational EvoDevo Group, Department of Computer Science, Leipzig University, Härtelstraße 16-18, 04107 Leipzig, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(15), 5210; https://doi.org/10.3390/ijms21155210
Received: 14 April 2020 / Revised: 20 July 2020 / Accepted: 22 July 2020 / Published: 23 July 2020
(This article belongs to the Section Molecular Biology)
Dictyostelium discoideum, the model organism for the evolutionary supergroup of Amoebozoa, is a social amoeba that, upon starvation, undergoes transition from a unicellular to a multicellular organism. In its genome, we identified two genes encoding for tRNA nucleotidyltransferases. Such pairs of tRNA nucleotidyltransferases usually represent collaborating partial activities catalyzing CC- and A-addition to the tRNA 3′-end, respectively. In D. discoideum, however, both enzymes exhibit identical activities, representing bona-fide CCA-adding enzymes. Detailed characterization of the corresponding activities revealed that both enzymes seem to be essential and are regulated inversely during different developmental stages of D. discoideum. Intriguingly, this is the first description of two functionally equivalent CCA-adding enzymes using the same set of tRNAs and showing a similar distribution within the cell. This situation seems to be a common feature in Dictyostelia, as other members of this phylum carry similar pairs of tRNA nucleotidyltransferase genes in their genome. View Full-Text
Keywords: tRNA nucleotidyltransferase; CCA-adding enzyme; enzyme evolution; Dictyostelium discoideum tRNA nucleotidyltransferase; CCA-adding enzyme; enzyme evolution; Dictyostelium discoideum
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Erber, L.; Hoffmann, A.; Fallmann, J.; Hagedorn, M.; Hammann, C.; Stadler, P.F.; Betat, H.; Prohaska, S.; Mörl, M. Unusual Occurrence of Two Bona-Fide CCA-Adding Enzymes in Dictyostelium discoideum. Int. J. Mol. Sci. 2020, 21, 5210.

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