Next Article in Journal
Lipidomic Biomarkers in Polycystic Ovary Syndrome and Endometrial Cancer
Next Article in Special Issue
Discovery of Novel Agents on Spindle Assembly Checkpoint to Sensitize Vinorelbine-Induced Mitotic Cell Death against Human Non-Small Cell Lung Cancers
Previous Article in Journal
YUCCA-Mediated Biosynthesis of the Auxin IAA Is Required during the Somatic Embryogenic Induction Process in Coffea canephora
Previous Article in Special Issue
Upregulation of Thr/Tyr kinase Increases the Cancer Progression by Neurotensin and Dihydropyrimidinase-Like 3 in Lung Cancer
Article

Reversible Growth-Arrest of a Spontaneously-Derived Human MSC-Like Cell Line

1
Biochemistry and Tumor Biology Lab, Department of Obstetrics and Gynecology, Hannover Medical School, 30625 Hannover, Germany
2
Department of Rheumatology and Clinical Immunology, Hannover Medical School, 30625 Hannover, Germany
3
Institute of Immunology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, 58453 Witten, Germany
4
Department of Toxicology, Hannover Medical School, 30625 Hannover, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(13), 4752; https://doi.org/10.3390/ijms21134752
Received: 26 May 2020 / Revised: 29 June 2020 / Accepted: 30 June 2020 / Published: 3 July 2020
(This article belongs to the Special Issue Cell Cycle and Cell Cycle Targeting Cancer Therapies)
Life cycle limitation hampers the production of high amounts of primary human mesenchymal stroma-/stem-like cells (MSC) and limits cell source reproducibility for clinical applications. The characterization of permanently growing MSC544 revealed some differentiation capacity and the simultaneous presence of known MSC markers CD73, CD90, and CD105 even after continuous long-term culture for more than one year and 32 passages. The expression of CD13, CD29, CD44, and CD166 were identified as further surface proteins, all of which were also simultaneously detectable in various other types of primary MSC populations derived from the umbilical cord, bone marrow, and placenta suggesting MSC-like properties in the cell line. Proliferating steady state MSC544 exhibited immune-modulatory activity similar to a subpopulation of long-term growth-inhibited MSC544 after 189d of continuous culture in confluency. This confluent connective cell layer with fibroblast-like morphology can spontaneously contract and the generated space is subsequently occupied by new cells with regained proliferative capacity. Accordingly, the confluent and senescence-associated beta-galactosidase-positive MSC544 culture with about 95% G0/G1 growth-arrest resumed re-entry into the proliferative cell cycle within 3d after sub-confluent culture. The MSC544 cells remained viable during confluency and throughout this transition which was accompanied by marked changes in the release of proteins. Thus, expression of proliferation-associated genes was down-modulated in confluent MSC544 and re-expressed following sub-confluent conditions whilst telomerase (hTERT) transcripts remained detectable at similar levels in both, confluent growth-arrested and proliferating MSC544. Together with the capability of connective cell layer formation for potential therapeutic approaches, MSC544 provide a long term reproducible human cell source with constant properties. View Full-Text
Keywords: mesenchymal stroma/stem cells; MSC marker; MSC cell line; reversible growth arrest; tissue regeneration mesenchymal stroma/stem cells; MSC marker; MSC cell line; reversible growth arrest; tissue regeneration
Show Figures

Figure 1

MDPI and ACS Style

Melzer, C.; Jacobs, R.; Dittmar, T.; Pich, A.; von der Ohe, J.; Yang, Y.; Hass, R. Reversible Growth-Arrest of a Spontaneously-Derived Human MSC-Like Cell Line. Int. J. Mol. Sci. 2020, 21, 4752. https://doi.org/10.3390/ijms21134752

AMA Style

Melzer C, Jacobs R, Dittmar T, Pich A, von der Ohe J, Yang Y, Hass R. Reversible Growth-Arrest of a Spontaneously-Derived Human MSC-Like Cell Line. International Journal of Molecular Sciences. 2020; 21(13):4752. https://doi.org/10.3390/ijms21134752

Chicago/Turabian Style

Melzer, Catharina, Roland Jacobs, Thomas Dittmar, Andreas Pich, Juliane von der Ohe, Yuanyuan Yang, and Ralf Hass. 2020. "Reversible Growth-Arrest of a Spontaneously-Derived Human MSC-Like Cell Line" International Journal of Molecular Sciences 21, no. 13: 4752. https://doi.org/10.3390/ijms21134752

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop