Next Article in Journal
Contribution of Immune Cells to Glucocorticoid Receptor Expression in Breast Cancer
Previous Article in Journal
miRNA Clusters with Down-Regulated Expression in Human Colorectal Cancer and Their Regulation
Open AccessArticle

New Insight into the Composition of Wheat Seed Microbiota

1
Department of Biology and Biotechnology of Microorganisms, The John Paul II Catholic University of Lublin, Konstantynów St. 1 I, 20-708 Lublin, Poland
2
Department of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation, Czartoryskich St. 8, 24-100 Puławy, Poland
3
Department of Plant Physiology and Biotechnology, The John Paul II Catholic University of Lublin, Konstantynów St. 1 I, 20-708 Lublin, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(13), 4634; https://doi.org/10.3390/ijms21134634
Received: 27 May 2020 / Revised: 28 June 2020 / Accepted: 29 June 2020 / Published: 30 June 2020
(This article belongs to the Section Molecular Microbiology)
Endophytes are associated with host plants throughout their life history from seed germination to fruit development. One of the most important plant organs colonized by endophytic microbiota is the seed. The aim of this study was to determine the structure of the seed core microbiome inhabiting the endosperms and embryos of eight wheat cultivars with the use of a culture-independent technique. The seeds of Triticum aestivum L. cv. Hondia, Wilejka, STH, Opcja, Tybalt, Euforia and Triticum spelta L. cv. Rokosz and Schwabencorn (producer: Plant Breeding Strzelce Sp. z o.o. Group IHAR) were studied. Rokosz and Hondia were cultured in vitro and in vivo to identify obligatory bacterial endophytes. A restrictive analysis of reads originating from the in vitro plants has demonstrated that the bacterial genera Paenibacillus and Propionibacterium inhabiting Rokosz and Hondia plants have a status of obligatory microorganisms. Greater biodiversity of seed-borne endophytes was found in the seed endosperms than in the embryos. The multiple comparison analysis of the OTU abundance indicated that the seed part significantly influenced the relative abundance. The seed-born microbiome is not statistically significantly dependent on the wheat cultivars; however, it cannot be claimed that every wheat seed is the same. View Full-Text
Keywords: endophytes; seeds; embryos; endosperm; wheat; core microbiome; NGS endophytes; seeds; embryos; endosperm; wheat; core microbiome; NGS
Show Figures

Figure 1

MDPI and ACS Style

Kuźniar, A.; Włodarczyk, K.; Grządziel, J.; Woźniak, M.; Furtak, K.; Gałązka, A.; Dziadczyk, E.; Skórzyńska-Polit, E.; Wolińska, A. New Insight into the Composition of Wheat Seed Microbiota. Int. J. Mol. Sci. 2020, 21, 4634.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop