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Article

Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients

1
Angiology and Vascular Surgery Service, La Fe University and Polytechnic Hospital, 46026 Valencia, Spain
2
Haemostasis, Thrombosis, Arteriosclerosis and Vascular Biology Research Group, Medical Research Institute Hospital La Fe (IIS La Fe), 46026 Valencia, Spain
3
Data Science, Biostatistics Unit, Medical Research Institute Hospital La Fe (IIS La Fe), 46026 Valencia, Spain
4
Genomic-Bioinformatic Unit, Medical Research Institute Hospital La Fe (IIS La Fe), 46026 Valencia, Spain
5
Department of Surgery, University of Valencia, 46010 Valencia, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(13), 4600; https://doi.org/10.3390/ijms21134600
Received: 19 May 2020 / Revised: 23 June 2020 / Accepted: 24 June 2020 / Published: 28 June 2020
(This article belongs to the Special Issue Molecular Research On Abdominal Aortic Aneurysm)
microRNAs (miRNAs) are small RNAs that regulate different biological processes. Our objective was to identify miRNAs dysregulated in plasma and tissue of patients with abdominal aortic aneurysm (AAA) and explore new potential targets involved in AAA. Fifty-seven subjects were recruited for a plasma study (30 AAA patients, 16 healthy volunteers and 11 patients with atherosclerosis). The expression level of 179 miRNAs was screened in plasma from a subset of samples, and dysregulated miRNAs were validated in the entire study population. Dysregulated miRNAs were also quantified in aortic tissue of 21 AAA patients and 8 organ donors. Applying a gene set enrichment analysis, an interaction map of dysregulated miRNAs and their targets was built, and selected targets were quantified in tissue samples. miR-27b-3p and miR-221-3p were overexpressed in plasma of AAA patients compared with healthy controls, 1.6 times and 1.9 times, respectively. In AAA tissue, six miRNAs (miR-1, miR-27b-3p, miR-29b-3p, miR-133a-3p, miR-133b, and miR-195-5p) were underexpressed from 1.6 to 4.8 times and four miRNAs (miR-146a-5p, miR-21-5p, miR-144-3p, and miR-103a-3p) were overexpressed from 1.3 to 7.2 times. Thrombospondin-2, a target of miR-195-5p, was increased in AAA tissue and negatively correlated with the expression of miR-195-5p, suggesting their involvement in a common regulatory mechanism. View Full-Text
Keywords: abdominal aortic aneurysm; microRNAs; miRNAs; biomarkers; Thrombospondin-2; vascular disease abdominal aortic aneurysm; microRNAs; miRNAs; biomarkers; Thrombospondin-2; vascular disease
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MDPI and ACS Style

Plana, E.; Gálvez, L.; Medina, P.; Navarro, S.; Fornés-Ferrer, V.; Panadero, J.; Miralles, M. Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients. Int. J. Mol. Sci. 2020, 21, 4600. https://doi.org/10.3390/ijms21134600

AMA Style

Plana E, Gálvez L, Medina P, Navarro S, Fornés-Ferrer V, Panadero J, Miralles M. Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients. International Journal of Molecular Sciences. 2020; 21(13):4600. https://doi.org/10.3390/ijms21134600

Chicago/Turabian Style

Plana, Emma, Laura Gálvez, Pilar Medina, Silvia Navarro, Victoria Fornés-Ferrer, Joaquín Panadero, and Manuel Miralles. 2020. "Identification of Novel microRNA Profiles Dysregulated in Plasma and Tissue of Abdominal Aortic Aneurysm Patients" International Journal of Molecular Sciences 21, no. 13: 4600. https://doi.org/10.3390/ijms21134600

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